TH31205C3 - L-glutamic acid producing bacteria and methods for producing L-glutamic acid - Google Patents

Abstract

DC60 (18/06/51) Method for the production of L-glutamic acid. It consists of a culture of the Klebsiella, Erwinia or Pantoea family and is capable of producing L-glutamic acid in the culture medium, and collection of L-glutamic acid produced from food. Cultivate Most microbial strains use Strains with reduced or impaired enzymatic catalytic activity that have branched off pathways for Biosynthesis of L-Glutamic acid and the production of other compounds. In addition to L-Glutamic acid, or strains with added enzyme-catalytic activity for biosynthesis, L-Glutamic acid method for L-Glutamic acid production. It consists of cultures of the Klebsiella family, Erwinia or Pantoea and is capable of producing L-glutamic acid in the culture medium, and collection of L-glutamic acid produced by the medium. Cultivate Most microbial strains use Strains with reduced or impaired catalytic enzyme activity branched off pathways for Biological synthesis L-glutamic acid And produce other compounds Other than L-glutamic acid, or strains that have been added to the enzyme activity Catalyst for L-Glutamic Acid Biosynthesis

Claims (8)

1. Method for L-Glutamic Acid Production, which consists of microorganism culture in Liquid culture medium to produce and accumulate extracellular L-glutamic acid in the germicidal medium. And L-glutamic acid collection from culture medium. The microorganisms belong to the Klebsidlla (Klebsidlla) family, Erwinia family or Pantoea family, and have been modified so that the microorganisms can function properly. This increases the amount of enzyme that catalyzes for L-glutamic acid biosynthesis. 2. Method according to claim 1 where microorganisms are klebsiella planticola or pantoea aggolmerans. 3. Method according to claim 1, whereby microorganisms have been modified so that microorganisms can be Enhanced work of one or more selected from a group containing vitrate syntase, phosphoenol pyruvate carboxylase, and glutamate dehydrate. Genes 4. Method according to claim 1, whereby microorganisms have been modified so that the microorganisms can be Increased functionality of vitrate syntase, phosphoenol pyruvate carboxylase and glutamate dehydrogenase. 5. Method according to claim 1, whereby microorganisms have been modified so that the microorganisms can be The reduced work of Or depletion of at least one of the enzymes selected from Group consisting of - Cytoglutarate dehydrogenase, isocitrate lyses, phosphate avatyltransferase, acetate kinase, acetate hydroxide. Zacid Syntase, Acetolactate Syntase, Formate Acetyltransferase, Lactate Dehydrogenase, A L-glutamate decarboxylase and 1-pyroline dehydrogenase 6. Method according to claim 5, whereby microorganisms are converted so that they can be The reduced work of Or depletion in the function of - - Kutoglutarate dehydrogenase 7. A method for the production of L-glutamic acid consisting of microorganisms belonging to the klebsiella family and capable of producing L-glutamic acid in the culture medium. Liquid to produce And L-glutamic acid accumulation outside the cell in the culture medium. And acid storage L-glutamic from aquaculture 8. Method according to claim 7, whereby klebsiella planticola is believed.