TH30801B - Production and use of thermally stable non-reductive saccharide enzymes - Google Patents

Abstract

A new heat-stable non-reducing saccharide enzyme has been revealed, its preparation and use. This enzyme is obtained from cell cultures of microorganisms such as Sulfolobus acidocaldarius (ATCC 33909 and ATCC 49426) and Sulfolobus solfataricus (ATCC 35091 and ATCC 35092) and can generate nonreducing. Saccharides with a trehalose structure form the end group when released to starch hydro. Reducing incomplete lysates at temperatures above 55 °C, glucoamylase and 4-glucidase readily yield trehalose upon release. Non-reducing saccharides Non-reducing saccharides and trehalose are very useful in food, cosmetic and pharmaceutical products, patent medicines.

Claims (6)

1.Process for the preparation of enzymes that produce non-redoxaccharides with trihalose structure. It is an endpoint unit and contains terehalose and glucose, without the need for trihalose as a substrate when it is released to the partial starch of rediclosates. Where the pure enzyme It was not inactivated at temperatures in the range of 55 ํ -70 ํ containing microorganisms that can produce the enzyme in the culture medium and store it. 2. Process of claim 1, where the microorganisms are members selected from the composite group. With those belonging to the genus Sulfolobus and its mutants 3. Process for creating non-redoxaccharides Which has a tertiose structure as an end-to-end unit and is composed of t-halo and glucose, which includes (a) the enzyme that produces non-redoxaccharides with a tree structure Halos As an end unit And consists of tetrahalose and glucose, without the need for trihalose as a substrate when allowed to The effect of the incomplete starch idsidolysate is redoxing. Where the pure enzyme Not made not It works at temperatures in the range of 55 ํ -70 ํ C. It is effective against a solution containing incomplete starch hydrolyzate. Reducing type is a substrate. And (b) purify the resulting non-reducing saccharide to obtain the non-reducing saccharide. 4. Process of claim 3 whereby Starch Hyolysate Imperfect redox Such is one or more redoxin incomplete starches that have the degree of glucose. Polymerization is at least 3. 5. The process of claim 3 The amount obtained in step (b) has been passed into the Collum chromatography using strong acidic cation exchange resin To add components of the bed Reducing saccharides. 6. Process for preparing saccharide compositions consisting of sleep preparation. Rewinding saccharides according to the method of claim 3 and then combining non-reducing saccharides in Other saccharides. 7. Processes for preparing food products that include the preparation of constituents. Saccharides following the procedure of claim 6 and then incorporating the saccharides into the food. 8. A cosmetic preparation process that includes the preparation of saccharide compositions. Follow the process of claim 6 and then incorporate the saccharide constituents into a cosmetic acceptable carrier. 9. Pharmaceutical preparation process that includes the preparation of saccharide constituents. In accordance with claim 6, and then combine the saccharide constituents into a pharmaceutical acceptable carrier 1 0. The process for preparing the terehalose that includes (a) provides the enzyme that produces the non-redirase. A zsaccharide with a tehalose structure as an end-to-end unit and consisting of trihalose and glucose, without the need for trihalose as a substrate when allowed to continue. Incomplete starchidlysate, redoxin Where the pure enzyme is not inactivated at temperatures in the range of 55 ํ -70 ํ C, it is effective against a starch hydrolyzate solution. Incomplete, redoxing type, is a substrate. In order to get non-reducing saccharides With a T-halo structure The distal and composed units of trehalose and glucosa (b) allow glucoamylase or - glucosidase to the resulting sleep reduction saccharide. To obtain trehalose and (c) collect the resulting solution with co-halose, starch hyrolysate, complete redoxing type 1. 1. Process of claim 10, whereby starch hydrolyzate is incomplete of such redoxing type. Selected member from a group containing incomplete, reddish-hydrolyzate starches. With the degree of Glucose polymerization at least 31 2. Process of claim 10, where TREEHalo is a member Selected from that group Contains hydrous terehalos seal. Anhydrous Terehalose Crystal And its mixes 1 3. Procedure of claim 10 where the solution obtained with the procedure (c) was introduced. It is passed into the chromatography column by using strong acid cation exchange resin to add trialose 1 component. 4. Processes for preparing food products that include preparation of trehalose according to Process of claim 10 and then incorporate trehalose into nutrient 1. 5.Cosmetic preparation process that consists of preparing trehalose according to the process Of claim 10, and then include trehalose into a cosmetic acceptable carrier 1 6. Pharmacopeia Preparation Process Combined with Processed Trialose Preparation Of claim 10 and then combine the trialos into a negotiable vehicle