TH22399B - Method for producing L-lysine - Google Patents

Abstract

DC60 (09/12/46) Coliniform bacteria with aspathodinase in which the reinhibition by L-lysine and L-threonine is reduced and their constituents are predominant. of the transcription DNA sequencing for dihydrodipicolinet reductase, predominance of the transcription strand for dihydrodipicolinate synthase, predominantly the sequence of DNA decoded for diaminopymilate decarboxylase, and predominance of DNA sequences decoded for aspartate aminotransferase, method of Produce L-lysine containing different steps of culturing coliniform bacteria in the medium. suitable culture In order to be produced and accumulated in bacterial culture and collection. L-lysine is extracted from culture and recombined DNA which can be used in the production of coliform bacteria Aspathodinase-containing colinidfor bacteria whose reactivation by lysine and lthreonine is reduced. and consists in the enrichment of the transcriptional DNA sequence for aspartodinase. where the re-inhibition with lysine and lthreonine was reduced, and the DNA sequencing decoded for dihydropicolinate synthase, the sequencing of the DNA Decoding for diamonopylates Decarboxylase and sequencing of DNA decoded for aspartate aminotransferase, a method for producing lysine containing different steps of coliniform bacterial culture in culture medium. suitable leaven In order to be produced and accumulated in the bacterial culture and the collection of L-lysine from the culture and recombined DNA which can be used in the production of coli bacteria. niform

Claims (12)

1. Recombinant DNA capable of automatic replication in colineform bacterial cells containing DNA sequences transcribed for aspartokines (which are reversibly inhibited by L-lysine and L-threonine), dihydrodipicolinate reductase, dihydrodipicolinate synthase, diaminopimylate decarboxylase, and aspartate aminotransferase. 2. Recombinant DNA pursuant to claim 1, where the aforementioned aspartokinesase, which is reversibly inhibited by L-lysine and L-threonine, is reduced to a colineform-derived aspartokinesase, and where the aforementioned aspartokinesase is a mutant aspartokinesase in which the amino acid region corresponding to the alanine region at position 279 from the nitrogenous end of the amino acid sequence shown in Sequence Table No. 5 is altered to an amino acid other than alanine and other than an acidic amino acid in its alpha subunit, and the amino acid region corresponding to the alanine region at position 30 from the nitrogenous end of its amino acid sequence shown in Sequence Table No. 7 is altered to an amino acid other than alanine and other than an acidic amino acid in its beta subunit. 3. Recombinant DNA pursuant to claim 1, whereby the aforementioned DNA sequence transcribed for dihydrodipicolinate reductase has been transcribed for the amino acid sequence shown in sequencing table number 11. 4. Recombinant DNA pursuant to claim 1, whereby the aforementioned DNA sequence transcribed for dihydrodipicolinate synthase transcribes the amino acid sequence shown in Synthesis Table No. 15. 5. Recombinant DNA pursuant to claim 1, whereby the aforementioned DNA sequence transcribed for diaminopimylate decarboxylase transcribes the amino acid sequence shown in sequencing table number 20. 6. Recombinant DNA pursuant to claim 1, whereby the referred DNA sequence transcribed for aspartate aminotransferase is transcribed for the amino acid sequences shown in Sequence Table No. 24 or 31. 7. Coliniform bacteria with aspartokines whose inhibition was reversibly triggered by L-lysine and L-threonine were downregulated, comprised of DNA sequences encoding dihydrodipicolinate reductase, dihydropicolinate synthase, diaminopimylate decarboxylase, and aspartate aminotransferase. The cellular interactions of dihydrodipicolinate reductase, dihydropicolinate synthase, diaminopimylate decarboxylase, and aspartate aminotransferase were dominant. 8. Colineform bacteria as defined in Claim 7, which have been altered by the introduction of recombinant DNA as defined in Claim 1. 9. A method for the production of L-lysine comprising the steps of culturing the coliniform bacteria as defined in claim 8 into a culture medium allowing L-lysine to be produced and accumulated in the bacterial culture medium, and the extraction of L-lysine from the culture medium. 10. DNA that has been isolated and transcribed for a protein consisting of the amino acid sequence shown in amino acid sequence table number 31. 11. DNA, according to claim 10, consists of the nucleotide sequences of nucleotides numbered 879 to 2174 in the nucleotide sequence shown in the sequencing table number 30. 12. The pVK7 carrier, which is autonomously replicable in Escherichia coli and Brevibacterium lactofermentum cells, contains multiple genetically designed loci and lacZ'.