TH18027C3 - Golden Cordyceps Cultivation Method by Liquid Fermentation for Cordycepin Production - Google Patents

Abstract

Summary of the invention which will be displayed on the ad page ReadFile:------18/10/2019------(OCR) Page 1 of 1 page. invention summary This invention relates to fermentation liquid fermentation culture of cordyceps for the production of cordycepin by stationary liquid culture consisting of inoculum preparation process in liquid medium. Shake the culture in stationary liquid medium without stirring or shaking and the use of inductor promotes the production of cordisipe in the fermentation by controlling the amount of the reactant and the filling interval. It is possible to increase both the fiber biomass and cordisipe content in this fermentation at high moisture content, which reduces the moisture content during the extraction of cordisipe from cordyceps fibers. Bring both the fermented water containing cordycepine and cordyceps fiber to be utilized as a component of food, dietary supplements and cosmetics-------------- Page 1 of 1 page invention summary This invention relates to Cultivation of golden cordyceps by liquid fermentation for the production of cordycepin by cultured in vitro liquid medium consisting of inoculum preparation process in liquid medium. Shaking culture in stationary liquid medium without stirring or shake and the use of inductor promotes the production of cordycepin in the fermentation water by controlling the amount of reactant and the appropriate filling interval. Both fiber biomass and cordycepin can be added to the fermentation in high moisture content, thus reducing the process of extracting cordycepin from cordyceps fibers. Fermented water containing cordycepin and cordyceps fiber is used as a food ingredient, dietary supplement and cosmetic product.

Claims (1)

Claims (all) which will not appear on the classifieds page:------18/10/2019------(OCR) Page 1 of 2 Claims Pages 1. The process of culturing golden knee beans by liquid fermentation for cordycepin production consists of A. Seedling preparations were made by culturing golden khoha fungi in liquid medium containing 15-25 g/l of carbon nutrient. Carbon nutrient can be selected from glucose and sucrose and 5-15 g/l of nitrogen nutrient. Nitrogen feed can be selected from peptone and yeast extract in a culture flask containing 30-40% of the volume of a dark incubation vial at 20-25°C and shaken at a speed of 120-150. rpm for 5-7 days B. The culture medium obtained from A.5-15 percent by volume of the culture liquid was transferred into a container containing the culture medium without the following components: carbon nutrients 15-25 grams per liter of carbon nutrients. Can be selected from glucose and sucrose and nitrogen nutrients 15-25 grams per liter, nitrogen nutrients can be selected from peptone and yeast extract, adjust pH at 5-6, close the lid tightly, then bring. Cultivated stationary without shaking under dark conditions at 20-25 degrees Celsius for 20-30 days. c. Add the inducer into the incubator bottle No. B. 10-30 grams per liter, then continue to culture in the dark at 20-25 degrees Celsius for 40-50 days. Production of cordycepin can be selected from xylose sugar. D. Take the culture flask obtained from the clause to separate the fermented water from the fibers by filtering to harvest the cordycepin containing fermented fermented broth------------ Page 1 of 2 pages of claims 1. Cultivation process of golden cordyceps by liquid fermentation for the production of cordycepin consists of a. Prepare the seedlings by culture Cordyceps fungi in liquid medium containing 15-25 g/l of carbon nutrient, carbon nutrient can be selected from glucose and sucrose and 5-15 g/l of nitrogen nutrient. Nitrogen feed can be selected from peptone and yeast extract in a culture flask containing 30-40% of the volume of the incubated vial in dark at 20-25°C and shaken at a speed of 120-150. rpm for 5-7 days B. The culture medium obtained from A.5-15 percent by volume of the agar liquid was transferred into a container containing the agar. The agar medium contains 15-25 grams of carbon per liter of carbon nutrients. Can be selected from glucose and sucrose and nitrogen nutrients 15-25 grams per liter, nitrogen nutrients can be selected from peptone and yeast extract, adjust pH 5-6, close the lid tightly, then bring to culture. stationary without shaking under knife conditions at a temperature of 20-25 degrees Celsius for 20-30 days c. Add the inducer into the incubator bottle No. B. 10-30 grams per liter, then continue to culture in the dark at 20-25 degrees Celsius for 40-50 days. Cordycepin production can be selected from xylose sugar. D. Take the culture flask obtained from the clause to separate the fermentation from the fiber by filtering to harvest the fermented fermented cordycepin. e. The content of cordycepin was analyzed in the filtered fermented water by HighPerformanceLiquidChromatography (HPLC). has a value greater than 1,700 milligrams per liter F. To analyze the fiber biomass of cordyceps, cordyceps fibers were washed at least 3 times with distilled water and dried by sublimation at -110 degrees Celsius until the fiber weight was stable.