CN113564094B - Method for increasing accumulated amount of mulberry Huang Baona polyphenol - Google Patents
Method for increasing accumulated amount of mulberry Huang Baona polyphenol Download PDFInfo
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Abstract
A method for increasing the intracellular polyphenol accumulation of Phellinus linteus by using unsaturated fatty acids, comprising: solid-cultured mulberry Huang Qiege is formed into small blocks, the small blocks are transferred into a fermentation culture medium, the small blocks are cultured in a constant-temperature shaking table, a shaking bottle is taken out, the small blocks are fully broken under the aseptic condition, and the small blocks are continuously placed in the constant-temperature shaking table for culture, so that mother liquor is used for subsequent liquid fermentation. Unsaturated fatty acid is added into the culture solution of the fermented Phellinus linteus. The invention uses unsaturated fatty acid as an exciton when Phellinus linteus is fermented and cultured, and can remarkably improve accumulation of Phellinus linteus biomass and yield of bristle extract polyphenol.
Description
Technical Field
The invention relates to the technical field of biological fermentation engineering, in particular to a method for improving the accumulation of polyphenol in Phellinus linteus cells by using unsaturated fatty acid.
Background
The medicinal fungi of Phellinus basidiomycota are valuable medicinal materials in China. Phellinus linteus is recorded in Ben Cao gang mu and has the functions of benefiting five viscera, xuan Chang stomach qi and expelling toxin. Researches show that the Phellinus linteus contains a plurality of active ingredients such as polysaccharide, triterpene, polyphenol, organic acid and the like, and the ingredients have remarkable activities in the aspects of resisting tumor, protecting liver, resisting oxidation, resisting bacteria, diminishing inflammation, enhancing organism immunity and the like [1-4]. The polyphenol compounds are composed of one or more hydroxyl groups, and are mainly classified into flavonoid compounds, pyrone compounds, furanone compounds and the like, and have higher antioxidant activity [5]. The flavonoid has antioxidant, antitumor, vasodilating and antibacterial effects; the pyrone compound has stronger capability of scavenging free radicals and biological activity of inhibiting NF- κB pathway; furanone compounds have antibacterial, antiviral and anticomplement activity [6-7].
Although wild-type Phellinus linteus can produce various active substances, in nature, the growth of Phellinus linteus is strict to the environment and fruiting bodies grow slowly. Along with the increasing market demand, wild resources are less and less, and artificial cultivation of Phellinus linteus becomes a focus of attention. However, under the condition of artificial fermentation culture, the accumulation amount of the secondary metabolite of Phellinus linteus is very low, and the production requirement cannot be met. This is because the genes involved in the synthesis of secondary metabolites from Phellinus linteus are all in a low-expression or silent state under artificial culture conditions. The research shows that after the fungi are stimulated by the external environment, the fungi can generate a series of response reactions, so that the biological expression level of genes is influenced, the enzyme activity is changed, and finally, the accumulation of secondary metabolites is improved.
The liquid fermentation culture has the characteristics of controllable conditions, short growth period and the like, however, the problem of low yield of active ingredients exists in the fermentation process of the phellinus linteus, so that how to increase the content of the active ingredients of the phellinus linteus by the exciton becomes an important research content under the condition of liquid fermentation.
Reference is made to:
1.Ge Qing,Mao Jianwei,Zhang Anqiang,Wang Yongjiang,Sun Peilong.Purification,chemical characterization,and antioxidant activity of a polysaccharide from the fruiting bodies of sanghuang mushroom(Phellinus baumii Pilát)[J].Food Sci Biotech,2013,22(2):384-389.
2.Yayeh T,Lee WM,Ko D,Park SC,Cho JY,Park HJ,Lee IK,Kim SH,Hong SB,Kim S,Yun BS,Rhee MH.Phellinus baumii ethyl acetate extract alleviated collagen type II induced arthritis in DBA/1mice[J].J Nat Med,2013,67(4):807-813.
3.Hwang BS,Lee IK,Choi HJ,Yun BS.Anti-influenza activities of polyphenols from the medicinal mushroom Phellinus baumii[J].Bioorg Med Chem Lett,2015,25(16):3256-3260.
4.Noh JR,Lee IK,Kim YH,Gang GT,Hwang JH,Ly SY,Yun BS,Lee CH.Antiatherogenic effect of antioxidant polyphenols from Phellinus baumii in apolipoprotein e-deficient mice[J].Ann Nutr Metab,2011,59(2-4):145-153.
5. Peak Bao Haiying, li Danhua "Phellinus Linteus" type fungus polyphenols and its biological activity research progress [ J ]. Natural products research and development 2016, (1): 147-155.
6. Huang Xiaowen, zhongming, dong Yu, etc. Phellinus linteus is used in ancient Chinese medicine books, and its modern pharmaceutical research progress [ J ]. Chinese journal of medicine, 2014,032 (009): 2249-2252.
7. Wu Changsheng research on the chemical composition of the medicinal fungus Sang Huanghua [ D ]. University of Shandong, 2011.
Disclosure of Invention
The invention aims to provide a method for improving intracellular polyphenol accumulation of fermentation-cultured phellinus linteus.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
a method for increasing the intracellular polyphenol accumulation of Phellinus linteus by using unsaturated fatty acids, comprising: unsaturated fatty acid is added to the culture medium of Phellinus linteus.
Further, the culture medium is a liquid culture medium.
Further, the ratio of the unsaturated fatty acid is 0.5g/L to 1g/L.
Further, the unsaturated fatty acid is linoleic acid and/or linolenic acid.
Further, the cultivation of Phellinus linteus specifically comprises:
inoculating solid cultured Phellinus linteus strain into liquid culture medium, performing activation culture, inoculating into seed fermentation culture medium according to 10% of the inoculating amount by volume percentage, performing seed culture, and performing shaking culture at constant temperature for 5 days to obtain seed liquid; inoculating the seed solution into a liquid fermentation culture medium according to the inoculum size of 5% by volume, adding unsaturated fatty acid to ensure that the concentration of the unsaturated fatty acid in the fermentation liquid is 0.5-1 g/L, culturing at constant temperature for 13 days, collecting a fermentation culture, and filtering to obtain mycelium.
Further, the rotation speed of the constant temperature shaking table culture is 150 revolutions per minute, and the culture temperature is 26 ℃.
Further, the seed culture medium is YPD culture medium, and the formula is as follows: 10 parts of yeast extract, 20 parts of peptone and 20 parts of glucose, and stirring thoroughly until dissolved.
Further, the formula of the liquid fermentation medium is as follows: mgSO was weighed 4 0.5 part (NH) 4 ) 2 SO 4 0.2 part, 20 parts of glucose and CaCl 2 0.1 part, yeast extract 10 parts, KH 2 PO 4 1 part of deionized water was added to the vessel, and after stirring until dissolved, the pH was adjusted to 5.5.
Compared with the prior art, the invention has the beneficial technical effects that:
the invention takes unsaturated fatty acid as an exciton when Phellinus linteus is fermented and cultured, and the accumulation of Phellinus linteus biomass and the yield of bristle-element polyphenols are obviously improved.
Drawings
FIG. 1 is the experimental results of the effect of linoleic acid on the biomass of the fermentation hyphae of Phellinus linteus;
FIG. 2 is the experimental results of the effect of linoleic acid on the polyphenols of Wannisang Huang Baona;
FIG. 3 is a graph showing experimental results of the effect of linoleic acid on the levels of three polyphenolic compounds;
FIG. 4 is the experimental results of the effect of linolenic acid on the biomass of the fermentation hyphae of Phellinus linteus;
FIG. 5 is the experimental results of the effect of linolenic acid on the polyphenols of Wannisang Huang Baona;
FIG. 6 is a graph showing the experimental results of the effect of linolenic acid on the contents of three polyphenol compounds.
Detailed Description
The invention is further illustrated below with reference to examples.
Example 1
1. Test strain and culture medium
Phellinus linteus (Sanghuangporus vaninii) strain is preserved in paraffin culture medium. Phellinus linteus liquid culture medium: mgSO (MgSO) 4 0.5g，(NH 4 ) 2 SO 4 0.2g, glucose 20g, caCl 2 0.1g, yeast extract 10g, KH 2 PO 4 1g in a 1L beaker, 800mL deionized water was added, and after stirring thoroughly until dissolved, the pH was adjusted to 5.5, and the volume was set at 1L. The solid culture medium is prepared by adding 15g of agar powder into each liter of liquid culture medium.
2. Liquid fermentation of Phellinus linteus
The solid cultured valnemia Huang Qiege was pelleted and transferred to fermentation media. In a constant temperature shaker, 150 revolutions per minute, at 26℃for 5 days. The shake flask was removed and broken up thoroughly with a hand-held homogenizer under sterile conditions. Placing in a constant temperature shaking table, culturing at 26 deg.C for 5 days at 150 rpm. Namely mother liquor. Can be used for subsequent liquid fermentation. Liquid fermented Phellinus linteus (S.vaninii) is divided into three groups, namely a control group containing no unsaturated fatty acid in the medium, and an experimental group containing 0.5g/L and 1g/L unsaturated fatty acid. Samples were taken at 5d, 7d, 9d, 11d and 13d of culture.
3. Determination of the amount of biomass
The fermentation culture was collected, filtered to obtain mycelia, and the mycelia were dried to constant weight and weighed.
4. Extraction of polyphenols
Filtering the fermentation broth, collecting mycelium, and placing into a centrifuge tube. 1 time of the cell volume of methanol was added to the centrifuge tube: acetone=1: 1, crushing the thalli by a flash extractor, fully contacting the thalli with the extracting solution, and soaking the thalli at room temperature for overnight. The sample tube was removed at 6000rmp, centrifuged for 5 minutes and the supernatant collected. Repeated three times. The supernatant was collected. The sample was concentrated using a rotary evaporator and dissolved with methanol.
5. Determination of polyphenol content
The polyphenol content is measured by using a Folin-Ciocalteu method by taking gallic acid as a standard substance, and a standard curve is drawn. Determination of polyphenol content in samples: and (3) measuring the polyphenol content in different samples by using a Folin-Ciocalteu method, substituting a standard curve equation, and calculating the polyphenol concentration of the samples.
6. Detection of polyphenol composition by HPLC method
The HPLC detection conditions were:
(1) Chromatographic column: atlantis dC 18.mu.m2.1X106 nm Column
(2) Chromatographic conditions: mobile phase: a:0.01% formic acid with 5% acetonitrile in water, B:0.01% acetonitrile formate; flow rate: 0.2mL/min; detection wavelength: 300nm; sample injection amount: 2. Mu.L; column incubator: 35 ℃; gradient elution procedure is as follows table 1:
TABLE 1 gradient elution procedure
Example 2: effect of linoleic acid on Phellinus Linteus biomass accumulation and polyphenol Synthesis
1. Variation of mycelium biomass
Adding different amounts of linoleic acid into Phellinus linteus liquid culture medium to make the concentration of linoleic acid in the culture medium respectively be: 0. 0.5g/L, and medium without linoleic acid is used as control. Both 0.5g/L and 1g/L linoleic acid can promote the increase of the biomass of the Phellinus linteus mycelium, and the mycelium growth speed at the concentration of 0.5g/L linoleic acid is the fastest in the fifth day of growth. With the increase of the culture time, the promoting effect of the concentration of 1g/L linoleic acid is more obvious after the seventh day, and the biomass is higher than that of the control group and 0.5g/L concentration. Until day 13, the concentration of linoleic acid was 1g/L, and the biomass of Phellinus linteus reached 6.9g/L, which was 53.33% higher than that of the control group (FIG. 1).
2. Variation of mycelium polyphenol accumulation
In the early stages of the growth of Phellinus linteus, linoleic acid showed no promoting effect on the accumulation of intracellular polyphenols. However, the intracellular polyphenol content of the experimental group to which linoleic acid was added was gradually higher than that of the control group (no linoleic acid was added) and the linoleic acid promoting effect was more remarkable at the concentration of 0.5g/L, and at the time of 11 days, the intracellular polyphenol content of the linoleic acid group at the concentration of 0.5g/L reached 5.37mg/g, which was 48.75% higher than that of the control group at the time of 3.61mg/g (FIG. 2).
3. Accumulation of the Compound Hispin
The difference of polyphenol composition under different culture conditions is detected by using an HPLC method by taking Hispin as a standard substance. As can be seen from FIG. 3, on days 5-7, the increase in Hispin content after treatment with 1g/L linoleic acid was relatively pronounced, with 0.5g/L and 1g/L concentrations reaching maximum values on days 9 and 13, 43.08. Mu.g/mg and 39.45. Mu.g/mg, respectively.
Example 2 Effect of linolenic acid on Phellinus Linteus biomass accumulation and polyphenol Synthesis
1. Effects on biomass accumulation
Different amounts of linolenic acid are added into the Phellinus linteus liquid culture medium, so that the concentration of the linolenic acid in the culture medium is respectively as follows: 0. 0.5g/L, and 1g/L of culture medium without linolenic acid is used as a control. Sampling according to growth time of mycelium, namely 5d, 7d, 11d and 13d, taking out 3 groups of samples, separating fermentation liquor and mycelium by filtration, fully drying, weighing dry weight of mycelium, recording, and finishing to obtain the chart 4. As can be seen from FIG. 4, the mycelium biomass increased at a smooth increase, with 0.5g/L and 1g/L linolenic acid added, compared to the control group without linolenic acid added. And on day 13, the dry weight of mycelium reaches the highest at each concentration, the control group is 3.99g/L, the 0.5g/L linolenic acid experimental group is 4.76g/L, and the 1g/L linolenic acid experimental group is 5.16g/L, wherein the growth promotion effect of the experimental group added with 1g/L linolenic acid is the best, and the dry weight of the experimental group is increased by 1.17g/L compared with that of the control group.
2. Changes in accumulation of polyphenols
On day 5, the total polyphenol content of the 0.5g/L experimental group was highest in the three groups of samples. The data from days 7 to 14 show that the experimental group with 1g/L linolenic acid added had the most pronounced promoting effect with increasing growth time, whereas the experimental group with 0.5g/L linolenic acid stimulated, although the content increased less than 1g/L, still increased compared to the control group. Eventually each group reached a maximum at day 13 and the total polyphenol content of both groups at this time with linolenic acid addition of 0.5g/L and 1g/L was approximately the same, increasing by 0.46mg/g compared to the content of the control group at this time (FIG. 5).
3. Accumulation of the Compound Hispin
FIG. 6 shows that starting on day 9, the Hispin content of the linolenic acid-added experimental group was higher than that of the control group, and that the linolenic acid-added experimental group with 0.5g/L was highest on day 13, reaching 8.17. Mu.g/mg.
Claims (4)
1. A method for increasing the intracellular polyphenol accumulation of Phellinus linteus by using unsaturated fatty acids, comprising:
inoculating solid cultured Phellinus linteus strain into liquid culture medium, performing activation culture, inoculating into seed fermentation culture medium according to 10% of the inoculating amount by volume percentage, performing seed culture, and performing shaking culture at constant temperature for a period of time to obtain seed liquid; inoculating the seed solution into a liquid fermentation culture medium according to an inoculum size with the volume percentage of 5%, adding unsaturated fatty acid to ensure that the concentration of the unsaturated fatty acid in the fermentation liquid is 0.5-1 g/L, culturing at constant temperature for 13 days, collecting a fermentation culture, and filtering to obtain mycelium; the unsaturated fatty acid is linoleic acid and/or linolenic acid.
2. The method according to claim 1, wherein the rotation speed of the constant temperature shaking culture is 150 rpm and the culture temperature is 26 ℃.
3. The method of claim 1, wherein the seed fermentation medium is YPD medium having the formula: 10 parts of yeast extract, 20 parts of peptone and 20 parts of glucose, and stirring thoroughly until dissolved.
4. The method of claim 1, wherein the liquid fermentation medium is formulated as follows: mgSO was weighed 4 0.5 part (NH) 4 ) 2 SO 4 0.2 part, 20 parts of glucose and CaCl 2 0.1 part, yeast extract 10 parts, KH 2 PO 4 1 part of deionized water is added into a container, and after the deionized water is fully stirred until the deionized water is dissolved, the pH value is adjusted to 5.5.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20160048342A (en) * | 2014-10-24 | 2016-05-04 | 강릉원주대학교산학협력단 | Novel cultivation method of mushroom |
| KR20180031094A (en) * | 2016-09-19 | 2018-03-28 | 전북대학교산학협력단 | Mushroom-fermented soybean using mushroom mycelium and method for manufacturing the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20160048342A (en) * | 2014-10-24 | 2016-05-04 | 강릉원주대학교산학협력단 | Novel cultivation method of mushroom |
| KR20180031094A (en) * | 2016-09-19 | 2018-03-28 | 전북대학교산학협력단 | Mushroom-fermented soybean using mushroom mycelium and method for manufacturing the same |
Non-Patent Citations (6)
| Title |
|---|
| A comprehensive study on phenolic compounds and bioactive properties of five mushroom species via chemometric approach;Cayan, F等;JOURNAL OF FOOD PROCESSING AND PRESERVATION;第45卷(第9期);e15695 * |
| Production of hispidin polyphenols from medicinal mushroom Sanghuangporus vaninii in submerged cultures;Wenwen Yuan等;Chinese Herbal Medicines;1-10 * |
| 不同脂肪酸对桑黄液体发酵的影响;高兴喜等;食品科学;第32卷(第7期);第1.2-1.3、2.1、2.4节 * |
| 桑黄发酵菌粉与桑黄子实体成分分析比较;王英辉等;食品与发酵工业;第242卷;126-129 * |
| 桑黄总黄酮超声提取工艺及其生物活性研究;回晶等;食品科学;第31卷;195-198 * |
| 桑黄活性成分的药理作用及人工栽培研究进展;陆春霞;董桂清;梁贵秋;潘志新;周晓玲;刘开莉;肖潇;黄正勇;吴婧婧;;广西蚕业(第04期);42-51 * |
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