TH17189U - A method for extracting compounds from pandan leaves that inhibit cholinesterase and/or beta-secretase enzymes. - Google Patents

Abstract

------23/03/2018------(OCR) Page 1 of 1 Abstract of the Invention The method for extracting compounds from pandan leaves that inhibit cholinesterase and/or beta-secretase enzymes, as described in this invention, consists of steps for preparing pandan leaves to reduce their size to improve extraction efficiency by freeze-drying, grinding them to a smaller size using a grinder, extraction of the compounds in different polar solvents, and testing to differentiate the size of the pandan leaves to improve the efficiency of inhibiting cholinesterase and/or beta-secretase enzymes. ------------ The method for extracting compounds from pandan leaves that inhibit cholinesterase and/or beta-secretase enzymes (formula-secretases), as described in this invention, consists of preparation steps... Pandan leaves were reduced in size to improve extraction efficiency by freeze-drying, grinding them into smaller pieces, and then using a grinder. Extraction processes were performed using different polar solvents, and size-coded pandan leaves were tested to enhance their inhibitory effect on cholinesterase and/or beta-secretase enzymes.

Claims (5)

------23/03/2561------(OCR) Page 1 of 1 page. Claims. 1. The extraction method for compounds from pandan leaves that inhibit cholinesterase and/or beta-secretase enzymes consists of the following steps: - Pandan leaves are prepared by reducing their size through freeze-drying, then ground into smaller pieces using a grinder, and stored at -20°C. - The dried pandan leaves are dissolved in a polar protic solvent at a ratio of pandan leaves to solvent of 1:20 by weight to volume, at 30°C for 15 minutes. - The mixture is then centrifuged at 3000 rpm for 10 minutes, and finally filtered using filter paper. 2. A method for extracting compounds from pandan leaves that exhibit inhibitory activity on cholinesterase and/or beta-secretase enzymes, as per claim 1, where the solvent can be selected from ethanol, methanol, and water. 3. Method for extracting compounds from pandan leaves that exhibit inhibitory activity on cholinesterase and/or betao-secretase enzymes (3-secretases) as per claim 1, where the optimal leaf size for extraction is between 0.13 mm and 0.42 mm. 1. The extraction method for compounds from pandan leaves that inhibit cholinesterase and/or beta-secretase enzymes consists of the following steps: - Pandan leaves are prepared by reducing their size through freeze-drying, then ground into smaller pieces using a grinder, and stored at -20°C. - The dried pandan leaves are dissolved in a polar protic solvent at a ratio of pandan leaves to solvent of 1:20 by weight to volume, at 30°C for 15 minutes. - The mixture is then centrifuged at 3000 rpm for 10 minutes, and then filtered using filter paper. 2. A method for extracting compounds from pandan leaves that exhibit inhibitory activity on cholinesterase and/or beta-secretase enzymes (formula-secretases) as per claim 1, where the solvent can be selected from ethanol, methanol, and water. 3. A method for extracting compounds from pandan leaves that exhibit inhibitory activity on cholinesterase and/or beta-secretase enzymes, as per claim 1, where the optimal leaf size for extraction is between 0.13 ml and 0.42 ml. 4. A method for extracting compounds from pandan leaves that exhibit inhibitory activity on cholinesterase and/or beta-secretase enzymes (formula-secretases) as per claim 1, where the resulting extract is a crude extract. 5. A method for extracting compounds from pandan leaves that exhibit inhibitory activity against cholinesterase and/or beta-secretase enzymes (formula-secretases) as per claims 1 through 5, in which the resulting pandan leaf extract exhibits inhibitory effect on the reduction of cholinesterase and/or beta-secretase enzymes (formula-secretases).