TH16869B - The process of extracting important substances from Phaya Yo And using extracts to produce mixtures for oral use, throat spray or external surface spray - Google Patents

Abstract

------ 19/03/2019 ------ (OCR) page 1 of number 1 page invention summary. The invention revealed the extraction process ofcomposition and bioactivity of Ciinacanthus nutans Lindau to increase the concentration and purity of the extract. (Or cleanliness of extracts according to traditional Thai traditional medicine) containing important substances, reduce the amount of colorant (colorant) in the extract to facilitate the preparation of a future product. The process of extracting important substances from Phaya Yo The steps are as follows. Extraction of Phaya yor leaf powder with ethanol, the removal of green substances from the extract. And preparation of dry Phaya Yo leaf extract Including the application of this Phaya Yor extract Application of Phaya Yor Extract contains a mixture of Phaya Yor extract for oral use with astringent effects. It is a ready-to-use gel, consisting of dry leaf extract, propylene glycol, glycerin, carboxymethylcellulose, xanthan gum, additive and additive. Choose from Sweetener Inhibitor of the growth of microorganisms, flavoring, phaya yor extract mixtures for intra-oral throat spray contains dry phaya yor leaf extract, glycerin, syrup, essential oil. And distilled water that has been sterilized Extracts from Phaya Yor for external surface spraying include Dried Phaya yor leaf extract, glycerin, essential oil And sterile distilled water ------------ ------ 30/08/2018 ------ (OCR) Page 1 of the number 1 page Summary of invention This invention reveals the extraction process of composition and bioactivity of Clinacanthus nutans Lindau to increase the concentration and Pure extracts (Or cleanliness of extracts according to traditional Thai traditional medicine) that contain important substances Reduce the amount of colorant in the extract to facilitate the preparation of a future product. The process of extracting important substances from Phaya Yo The steps are as follows. Extraction of Phaya yor leaf powder with ethanol Removing green substances from the extract And preparation of dry Phaya Yo leaf extract Including the application of Phaya Yor Extract This application of Phaya Yor Extract contains a mixture of Phaya Yor extract for use in Oral cavity with an astringent effect It is gel-like, ready-to-use, contains extracts of dry leaves, propylene glycol, glycerin, carboxymethylcellulose, xanthan gum, additives and water by additives. Choose from a sweetener Inhibitor of the growth of microorganisms, flavoring, phaya yor extract mixtures for intra-oral throat spray contains dry phaya yor leaf extract, glycerin, syrup, essential oil. And distilled water that has been sterilized Phaya Yo Extract Mixture for external surface spraying Contains dry Phaya yor leaf extract, glycerin, essential oil. And sterile distilled water ------------ Page 1 of 1 page. Summary of invention This invention reveals the extraction process of composition and bioactivity of Ciinocanthus nutans Lindau to increase the concentration and Pure extracts (Or cleanliness of extracts according to traditional Thai traditional medicine) containing important substances reduce the amount of colorant (colorant) in the extract to facilitate the preparation of a future product. The process of extracting important substances from Phaya Yo The steps are as follows. Extraction of Phaya yor leaf powder with ethanol Removing green substances from the extract And preparation of dry Phaya Yo leaf extract Including the application of Phaya Yor Extract This application of Phaya Yor Extract contains a mixture of Phaya Yor extract for use in Oral cavity with an astringent effect It has a gel appearance, ready to use. Phaya Yo Extract Mixture for intra-oral throat spray Mix Phaya Yor extract for external skin spray

Claims (9)

Disclaimer (all) which will not appear on the advertisement page. : ------ 19/03/2019 ------ (OCR) Page 1 of 2 pages. 1. Process for extracting important substances from Phaya Yo The steps are as follows: A. Extraction of Phaya yor leaves with ethanol. The leaves of Phaya Yo that have been dried to a moisture content of 2 to 10 percent are blended into a fine powder with a blender. Until getting fine Phaya yor leaf powder, size 60 to 1000 micrometers; b. Dried Phaya Yor leaf powder extracted with ethanol-water solution Contains 70 to 95% ethanol concentration by volume by volume. The ratio by weight of dried paya yor leaves to ethanol volume was 2 to 4 per 10 in the extraction container. Soak for 2 to 7 days in the extraction condition without light at room temperature, stirring well every day. C. Remove the mixture according to Clause B, separate the extract solution. Out of the Phaya Yor leaf powder Through filter paper with pore size of 2.5 to 25 μm. D. The extract was evaporated by ethanol by evaporation distillation under vacuum pressure until the liquid was 5 to 30% of the volume. Starting in item B.J., the removal of green substances from Phaya Yo leaf extract By the liquid phase extraction method, the extracts from the evaporated Phaya Yor leaves were removed from ethanol. It was dissolved with methanol solvent until the methanol concentration was 40 to 60 percent by volume by volume. Then add the extract to the separator funnel f. Add the hexane solvent to the solution in Article E in the same volume as the methanol extract solution. Shake for 5 to 10 minutes to combine the hexane. Leach the green substance from the methanol layer and let it sit for 10 to 15 minutes to allow the solution to separate. Keep the solution in the methanol layer. Which the extract is dissolved. Ch. Leading solvent, methanol To evaporate methanol out By using the distillation to evaporate under vacuum pressure until 40 to 60 percent of the liquid from the initial volume in Section E, which will get a solution of Phaya Yor leaf extract dissolved in water. G. To dissolve with ethanol. Then mix them together until the ethanol concentration is 70 to 90% by volume-by-volume solution. I. Remove the extracted solution obtained in Article C to evaporate the ethanol under the Push the vacuum until 10 to 30 percent of the extract from the initial volume in item C, which will get a 2-page solution of paya yor leaves that dissolve in water. The steps that H to I. can be done more than once. Phaya Yo leaf extract and Nam. J. Dried Phaya Yo leaf extract preparation. The solution of Phaya Yor leaf extract from the step I was to evaporate the water until dry. With freeze drying Get a product of dry Phaya Yo leaf extract 2. Process of extracting important substances from Phaya Yo According to claim 1, which is supplemented by Step A. Extending the retention period Extract constant By hardening and dissolving the dried Phaya Yo leaf extract to melt the water Bring to evaporate the water until dry By drying the freeze until the extract is dried. B. Take samples of dried Phaya Yo leaf extract to sterilize. Then stored in a desiccant at room temperature ------------ ------ 30/08/2018 ------ (OCR) page 1 of number 2. Claim page 1. Process for extracting important substances from Phaya Yo The steps are as follows: A. Extraction of Phaya yor leaves with ethanol. The leaves of Phaya Yo that have been dried to a moisture content of 2 to 10 percent are blended into a fine powder with a blender. Until obtaining fine Phaya Yor leaf powder, about 60 to 1000 micrometers in size. B. Dried Phaya Yor leaf powder extracted with ethanol-water solution. Contains 70 to 95% ethanol concentration by volume by volume. The ratio by weight of dried paya yor leaves to ethanol volume was 2 to 4 per 10 in the extraction container. Soak for 2 to 7 days in the extraction condition without light at room temperature, stirring well every day. C. Remove the mixture according to Clause B, separate the extract solution. Out of the Phaya Yor leaf powder Through filter paper with pore size of 2.5 to 25 μm. D. The extract was evaporated by ethanol by evaporation distillation under vacuum pressure until the liquid was 5 to 30% of the volume. Starting in item B.J., the removal of green substances from Phaya Yo leaf extract By the liquid phase extraction method, the extracts from the evaporated Phaya Yor leaves were removed from ethanol. It was dissolved with methanol solvent until the methanol concentration was 40 to 60 percent by volume by volume. Then add the extract to the separator funnel f. Add the hexane solvent to the solution in Article E in the same volume as the methanol extract solution. Shake for 5 to 10 minutes to combine the hexane. Leach the green substance from the methanol layer and let it sit for 10 to 15 minutes to allow the solution to separate. Keep the solution in the methanol layer. Which the extract is dissolved. Ch. Leading solvent, methanol To evaporate methanol out By using the distillation to evaporate under vacuum pressure until 40 to 60 percent of the liquid from the initial volume in Section E, which will get a solution of Phaya Yor leaf extract dissolved in water. G. To dissolve with ethanol. Then mix them together until the ethanol concentration is 70 to 90% by volume-by-volume solution. I. Remove the extracted solution obtained in Article C to evaporate the ethanol under the Push the vacuum until 10 to 30 percent of the extract from the initial volume in item C, which will get a 2-page solution of paya yor leaves that dissolve in water. The steps that H to I. can be done more than once. Phaya Yo leaf extract and Nam. J. Dried Phaya Yo leaf extract preparation. The solution of Phaya Yor leaf extract from the step I was to evaporate the water until dry. With freeze drying Get a product of dry Phaya Yo leaf extract 2. Process of extracting important substances from Phaya Yo According to claim 1, which is supplemented by Step A. Extending the retention period Extract constant By hardening and dissolving the dried Phaya Yo leaf extract to dissolve in water Bring to evaporate the water until dry By freeze drying until the dry extract was obtained. B. Samples of dried Phaya Yo leaf extract were sterilized. Then stored in a desiccant at room temperature ------------ Page 1 of 3 pages. 1. Process for extracting important substances from Phaya Yo The steps are as follows: A. Extraction of Phaya Yor leaves with ethanol b. Elimination of green substances from Phaya Yor leaf extract. By means of extraction by liquid phase. C. Preparation of dry Phaya yor leaf extract. 2. Process of extracting important substances from Phaya Yo According to claim 1, where the extraction of Phaya Yor leaves with ethanol, the steps are as follows: A. Take the dried Phaya Yo leaves until moisture content of approximately 2 to 10 percent is blended into a fine powder. With a blender Until obtaining fine Phaya Yor leaf powder, about 60 to 1000 micrometers in size. B. Dried Phaya Yor leaf powder extracted with ethanol-water solution. Contains 70 to 95% ethanol concentration by volume by volume. The ratio by weight of dried paya yor leaves to ethanol volume was 2 to 4 per 10 in the extraction container. Soak for 2 to 7 days in the extraction condition without light at room temperature, stirring well every day. C. Remove the mixture according to Clause B, separate the extract solution. From the Phaya Yor leaf residue through a filter paper with a pore size of 2.5 to 25 micrometers; d. The extract was evaporated to evaporate ethanol by evaporating under vacuum pressure until the liquid was left. The volume is 5 to 30 percent from the initial volume in item B. 3. Process for extracting important substances from Phaya Yo According to claim 1 or 2, where the elimination of green substances from Phaya Yor leaf extract By means of extraction by liquid phase The steps are as follows: a. Remove the extract from the evaporated Phaya yor leaves with ethanol. It was dissolved with methanol solvent until the methanol concentration was 40 to 60 percent by volume by volume. Then add the extract to the separation funnel. B. Add hexane solvent to the solution in Article A in a volume equal to the methanol extract solution. Shake for 5 to 10 minutes to combine the hexane. Leach the green substance from the methanol step, let it sit for 10 to 15 minutes to separate the solution. Store the methanol solution. Which the extract dissolves on page 2 of 3 pages c. Leading solvent, methanol To evaporate methanol out By using the distillation to evaporate under vacuum pressure until 40 to 60 percent of the liquid from the initial volume in item A. This will get a solution of Phaya Yor leaf extract dissolved in water. D. C. To dissolve with ethanol Then mix them together until the ethanol concentration is 70 to 90 by volume by volume. E. Remove the extract solution obtained in Section D. Evaporate the ethanol under the Push the vacuum until an extract with a volume of 10 to 30% from the initial volume in item D is obtained. Extracted from Phaya Yor leaves dissolved in water. Which steps D to Mon can be done more than once, Phaya Yor leaf extract and water 4. Process of extracting important substances from Phaya Yo According to the claims 1 to 3, whichever is the preparation of dry Phaya yor extract By using a solution of Phaya Yor leaf extract According to the claim that 3. E. To evaporate the water to dry with freeze drying. Get a product of dry Phaya Yo leaf extract 5. Process for extracting important substances from Phaya Yo According to claim 4, which is supplemented by Step A. Extending the retention period Extract constant By hardening and dissolving the dried Phaya Yo leaf extract to dissolve in water Bring to evaporate the water until dry By freeze drying until the dry extract was obtained. B. Samples of dried Phaya Yo leaf extract were sterilized. Then stored in a desiccant at room temperature. 6. Phaya Yor extract mixture for oral use. Containing Dried parsley leaf extract 0.5% to 5% by w / w 5% to 15% propylene glycol by w / w 15% to 25% glycerin by weight / wt. 1 to 5% carboxymethylcellulose by wt / wt, 1% to 3% xanthan gum by wt / additive weight 0.01% to 1.7% by weight / wt. 60% water. To 77 by weight / 3 page weight of 3 pages. 7. Phaya Yor extract mixture for oral use According to claim 6, additives are selectable from or composed of 0.01% to 0.05% sodium saccharin by wt / wt and / or xylitol 0.01% to 0.05% w / wt and / or. Essential oils 0.5% to 1.5% w / w and / or microbial growth inhibitor 0.01% to 0.05% w / w. 8. Mixture of Phaya Yor extract for intra-oral throat spray. Containing Dried Phaya yor leaf extract 0.5 to 4 g, 10 to 30 ml glycerin, 10 to 50 μl syrup, 0.5 to 2 ml essential oil, sterile distilled water. Fill until the volume is 100 ml. 9. Mixture of Phaya Yor extract for external surface spraying Containing Dried Phaya yor leaf extract 0.5 to 4 g. Glycerin 10 to 30 ml. Essential oil 0.5 - 2 ml. Distilled water sterile. Fill until the volume is 100 ml.