TH1643C3 - "Solid microorganism culture food formula without using heat - Google Patents

Abstract

The invention is the introduction of alginic acid into a gel-like substance without the use of agar. Gel occurrence of Alginate does not require heat, so it can be prepared without a heating device. And can be prepared Not complicated manually To be used during field operations for microbial counting. Can be present in food and environment, so it is likely to be of great benefit in the food manufacturing industry. And food processing This invention has developed a solid sterile food formula without using heat and a method of sterilization using Developed germicidal food recipes Especially the use of alginate as an F-500

Claims (3)

1. Food gels Which contains alginate Sodium hexametaphosphate (Na hp), triptone, yeast extract, and glucose are the main components. 2. Refined food gel formulated according to claim 1, where alginate is F-500 at a concentration of 20 g / l, sodium hexametaphosphate (Na hp) at a concentration of 2 g. Per liter of trypton at a concentration of 5 g / l; yeast extract At a concentration of 2.5 g / l and glucose at a concentration of 1 g / l 3. Methods for culturing microorganisms: 15 ml volume of germicidal food gel according to item 1 or item 2 is placed in a sterile beaker. Add 1 ml of the diluted solution of the sample to be tested for contamination in the same beaker. Add a sterile solution of calcium sulphate at a concentration of approximately 1.5% continuously stirred in the volume of 2.25 ml to the same beaker. Pour the prepared solution onto a plate. The sterile culture was then incubated at room temperature for 24 to 48 hours or until the microorganism was counted.