TH1509C3 - Solid microorganisms without heat - Google Patents

Abstract

This invention was the development of a solid without heat culture medium. By using F-200 alginate as the main ingredient To make it possible to prepare the agar without the use of heat, the agar can be prepared manually by a hassle-free method. Which can be used during field operations. For counting the number of microorganisms that contaminate food and the environment, it is likely to be particularly useful in the For food production And food processing

Claims (2)

1. Gel, which contains microorganisms. F-200 alginate at a concentration of 30 g / l, sodium hexametaphosphate (Na hp) at a concentration of 3 g / l, trypton at a concentration of 5 g / l, yeast ex-compound. T At 2.5 g / l and glucose at 1 g / l as the main component. 2. Microbial culture method: 15 ml volume of culture gel according to claim No. 1 is placed in a sterile beaker. Add 1 ml of the diluted solution of the sample to be tested for contamination in the same beaker. Add sterile calcium sulfate solution. At a concentration of approximately 2.0% the person was constantly in the volume of 2.25 ml into the same beaker. Pour the prepared solution onto a plate. The sterile culture was then incubated at room temperature for 24 to 48 hours or until the microorganism was counted.