TH15211Y - Method for extracting saponins from Bacopa monnieri. - Google Patents

Abstract

------12/07/2561------(OCR) Page 1 of 1. Abstract of the Invention: This invention concerns the mixture and method for extracting saponins from Bacopa monnieri. Dried Bacopa monnieri is fermented with 70% ethanol in a percolator fermenter, repeated twice. The extract is then concentrated by evaporation under vacuum conditions. A greenish-brown extract is obtained, rich in saponins, to enhance its active ingredients in dietary supplements, herbal medicines, and cosmetics.

Claims (1)

------12/07/2561------(OCR) Page 1 of 1 page. Claims 1. The method for extracting saponins from Bacopa monnieri, as described in this invention, consists of the following steps: a. Mix 5.96-7.24 kg of ethanol (optimal amount 7.24 kg) with 2.92-3.55 kg of water (optimal amount 3.55 kg). Divide the mixed ethanol into two parts. b. Ferment the first part of the mixed ethanol from step a with 1 kg of dry Bacopa monnieri in a percolator for 24 hours. After the time is up, filter and collect the first extract, and keep the Bacopa monnieri residue for use in the next extraction step. c. Mix the second part of the mixed ethanol from step a with the Bacopa monnieri residue from step b and ferment in a percolator for 24 hours. After the time is up, Filter and collect the second extract. Mix the first and second extracts thoroughly. Evaporate the extracts under vacuum conditions at a temperature of 35-50°C (optimal 40°C) and a pressure of 600-700 mmHg (optimal 650 mmHg) until a concentrated, brownish-green extract is obtained. ------------ ------26/01/2018------(OCR) Page 1 of 1 page. Claim 1. The formula and method for extracting saponins from Bacopa monnieri as per this invention. The process consists of the following steps: (a) Mix 5.96-7.24 kg of ethanol (optimal amount 7.24 kg) with 2.92-3.55 kg of water (optimal amount 3.55 kg). Divide the ethanol mixture into two equal parts. (b) Ferment the first part of the ethanol mixture from step (a) with 1 kg of dried Bacopa monnieri in a percolator for 24 hours. After the time is up, filter and collect the first extract, and keep the Bacopa monnieri residue for use in the next extraction step. (c) Mix the second part of the ethanol mixture from step (a) with the Bacopa monnieri residue from step (b) and ferment in a percolator for 24 hours. After the time is up, filter and collect the second extract. (d) Mix the first and second extracts thoroughly. The obtained extract was evaporated under vacuum conditions at a temperature of 35-50°C and a pressure of 600-700 mmHg, with the best concentration at 40°C and a pressure of 650 mmHg, until a concentrated, greenish-brown extract was obtained. ------------ Page 1 of 1 page. Claims. 1. The formula and method for extracting saponins from Bacopa monnieri, as described in this invention, consist of the following steps: (a) Mix 5.96-7.24 kg of ethanol (optimal amount 7.24 kg) with 2.92-3.55 kg of water (optimal amount 3.55 kg). Divide the ethanol mixture into two equal parts. (b) Ferment the first part of the ethanol mixture from step (a) with 1 kg of dried Bacopa monnieri in a percolator for 24 hours. After the time is up, filter and collect the first extract, and keep the Bacopa monnieri residue for use in the next extraction step. (e) Mix the second part of the ethanol mixture from step (b) with the Bacopa monnieri residue from step (b) and ferment in a percolator for 24 hours. After the time is up, filter and collect the second extract. (h) Mix the first and second extracts thoroughly. The obtained extract was evaporated under vacuum conditions at a temperature of 35-50 degrees Celsius and a pressure of 600-700 millimeters of mercury, with the best concentration at 40 degrees Celsius and a pressure of 650 millimeters of mercury, until a concentrated, greenish-brown extract was obtained.