TH117366A - A process for creating a double RNA by a two-step cloning system. - Google Patents

Abstract

------06/11/2020------(OCR) This invention is about a process for producing long double-stranded RNA. It uses a carrier DNA that contains a fragment of template DNA to form a hairpin RNA strand and transfers the target gene into host cells to be used as a source for producing large-scale RNA patterns. This method simplifies the steps of inserting the template DNA of the target gene into the carrier DNA by using a template DNA strand that is approximately 100 bases longer than the reverse strand, resulting in the excess of the template strand becoming a hairpin RNA curve. This does not require additional cloning of unrelated genes as with the previous method. Since the gene editing process can be done in two steps, the number of DNA primers required in this method is halved compared to the previous method and the double-stranded RNA that is specific to the base sequence in the yellow head virus dependent RNA polymerase gene can be used to effectively prevent yellow head virus disease in shrimp. ------------ DC60 (26/05/54) This invention is about a process for producing long double-stranded RNA. The carrier DNA contains a fragment of template DNA to be converted into a hairpin RNA strand, and the vector DNA containing the target gene is transfected into host cells to serve as a source for high-throughput RNA production. This method simplifies the insertion of the template DNA of the target gene into the carrier DNA by using a template DNA strand approximately 100 bases longer than the reverse strand, resulting in the excess portion of the template strand becoming a hairpin RNA curve without the need to clone additional unrelated genes as with the conventional method. Since the gene editing process can be performed in two steps, the number of DNA primers required in this method is halved compared to the conventional method. Furthermore, double-stranded RNA, specifically designed to target the base sequence of the yellow head virus (YHV) dependent RNA polymerase gene, can be effectively used to prevent yellow head virus disease in shrimp.

Claims (1)

1. : DC60 (26/05/54) This invention relates to a method for producing long double-stranded RNA by using a carrier DNA containing a fragment of template DNA to form a hairpin RNA strand and transferring the vector DNA containing the target gene into host cells for use as a source for producing large quantities of RNA strands. This method simplifies the steps of inserting the template DNA of the target gene into the carrier DNA by using a template DNA strand approximately 100 bases longer than the reverse strand, resulting in the excess of the template strand becoming a curved hairpin RNA without the need to clone additional unrelated genes as with the conventional method. Since the gene editing process can be done in two steps, the number of DNA primers required in this method is halved compared to the conventional method. And the double-stranded RNA that is specific to the base sequence in the yellow head virus dependent RNA polymerase gene can be used to effectively prevent yellow head virus disease in shrimp. Claim (first item) which will appear on the advertisement page: Tag: