TH10327B - ???????????????????????????????????????????????????? L-????? - Google Patents

Abstract

???? L-???????????????????????????? ??????????????????????????????????????????? Escherichia ?????????????????????????????????????????????????????????? ????????????????? ????????????? L-????? ????????????????????? Escherichia ????????????????????????????????? ???????????????????????????????????????? ???/???? ??? cadA ?????????????? ???????????????? L-??????????????????????????????

Claims (22)

1. A cleaved nucleic acid molecule that encodes lysine decarboxylase, where lysine decarboxylase consists of the amino acid sequence SEQ ID NO.4. 2. The isolated nucleic acid molecule of claim 1, comprising the sequence corresponding to positions 1005 to 3143 of SEQ ID NO.3. 3. Isolated microorganisms of the Escherichia genus that possess a mutation in the original gene transcribing primitive lysine decarboxylase; these microorganisms lack the original gene transcribing primitive lysine decarboxylase, which consists of the amino acid sequence SEQ ID NO. 4; or they have a non-transcribing mutated lysine decarboxylase gene that performs decarboxylation; or they have a non-transcribing lysine decarboxylase mutated gene that performs less decarboxylation than primitive lysine decarboxylase; or the mutated gene has a mutation in the control region, causing the microorganism to produce less primitive lysine decarboxylase than microorganisms with the original gene transcribing primitive lysine decarboxylase. 4. Isolated microorganisms under claim 3 where the mutated gene has a mutation in a regulatory region that causes the microorganism to produce less of the original lysine decarboxylase than microorganisms with the original gene that transcribes the original lysine decarboxylase. 5. The isolated microorganism under claim 3 belongs to the species Escherichia coli. 6. The isolated microorganism of claim 3, where the primitive gene consists of sequences corresponding to positions 1005 to 3143 of SEQ ID NO.3. 7. An isolated microorganism under claim 3 where the mutated gene does not transcribe the decarboxylase-dependent lysine-decarboxylate. 8. An isolated microorganism under claim 3 where a mutated gene encodes a lysine-decarboxylase mutant with less decarboxylation function than the original lysine-decarboxylase. 9. An isolated microorganism under claim 3 is characterized by a second mutation of the second primordial gene transcribing lysine decarboxylase II. This microorganism lacks the second primordial gene transcribing lysine decarboxylase II. Lysine decarboxylase II comprises the amino acid sequence of SEQ ID NO. 6, and the second mutation does not transcribe decarboxylated lysine decarboxylase; the second mutation transcribes less decarboxylated lysine decarboxylase II than the original lysine decarboxylase II; and the second mutation contains a mutation in the control region, resulting in microorganisms producing less lysine decarboxylase II than microorganisms with the second primordial gene transcribing lysine decarboxylase II. 10. An isolated microorganism under claim 9 where the second mutant gene is mutated in the regulatory region, causing this microorganism to produce less primitive lysine decarboxylase II than microorganisms with the second primitive gene that transcribes primitive lysine decarboxylase II. 11. An isolated microorganism under claim 9 where the second mutated gene does not transcribe decarboxylate-functioning lysine decarboxylase. 12. An isolated microorganism under claim 9 where a second mutant gene encodes a second mutant lysine decarboxylase with less decarboxylation function than the original second lysine decarboxylase. 13. A method for the production of L-lysine consisting of (a) culturing of the microbial trait 3 in a liquid medium in which L-lysine is produced and accumulated in the liquid medium, and (b) collecting the L-lysine produced and accumulated in step (a). 14. Method of Claim 13, where a mutated gene in the regulatory region causes microorganisms to produce less of the original lysine decarboxylase than microorganisms with the original gene transcribing the original lysine decarboxylase. 15. Method of Claim 13, where the microorganism is of the species Escherichia coli. 16. Method of Claim 13, where the primitive gene consists of sequences corresponding to positions 1005 to 3143 of SEQ ID NO.3. 17. Method of Claim 13 where the mutated gene does not transcribe lysine decarboxylase which has decarboxylate function. 18. Method of Claim 13, where the mutated gene transcribes lysine decarboxylase mutations that have less decarboxylate function than the original lysine decarboxylase. 19. Method of Claim 19: Where the microorganism possesses a second mutant gene of the second original gene transcribing lysine decarboxylase II; the microorganism lacks a second original gene transcribing lysine decarboxylase II; lysine decarboxylase II consists of the amino acid sequence SEQ ID NO. 6; and the second mutant gene does not transcribe lysine decarboxylase with decarboxylation function; the second mutant gene transcribes lysine decarboxylase II with less decarboxylation function than original lysine decarboxylase II; or the second mutant gene has a mutation in the control region that causes the microorganism to produce less lysine decarboxylase II than microorganisms with the second original gene transcribing lysine decarboxylase II. 20. The method of claim 19, where the second mutant gene has a mutation in the control region, resulting in microorganisms producing less primitive lysine decarboxylase II than microorganisms with a second primitive gene that transcribes primitive lysine decarboxylase II. 21. Method of claim 19 where the second mutated gene does not transcribe the decarboxylase lysine that performs decarboxylation function. 22. The method of claim 19, where the second mutant gene transcribes the second mutant lysine decarboxylase, which has less decarboxylate function than the second original lysine decarboxylase.