TH10170Y - A nutrient formula that induces the growth of complete plants in tissue culture, containing both upside-down and right-side-up leaves as components. - Google Patents

Abstract

DC60 (15/09/57) A study of culture medium formulas that induce the formation of complete plants in tissue cultures containing *Bauhinia acuminata* leaves as a component found that the best culture medium was MS. Chemicals used for preparing the MS culture medium: _____________________________________________________________________________________________________________________ Type, Quantity according to standard formula Amount in concentrated solution (mg/L) 100 times (g/L) Main nutrient solution MS CaCl12.2H2O (Calcium chloride) 440.00 44 KNO3 (Potassium nitrate) 1900.00 190 KH2PO4 (Potassium dihydrophosphate) 170.00 17 MgSO4.7H2O (Magnesium sulfate) 370.00 37 NH4NO3 (Ammonium nitrate) 1,650.00 165 Secondary nutrient solution MS MnSO4.4H2O (Manganese sulfate) 16.90 1.69 ZnSO4.7H2O (Zinc sulfate) 8.60 0.86 H3BO3 (Boric acid) 6.20 0.62 KI (Potassium iodide) 0.83 0.083 Na2MoO4.2H2O (Sodium molybdate) 0.25 0.025 CoCl2.6H2O (Cobalt chloride) 0.025 0.0025 CuSO4.5H2O (Copper sulfate) 0.025 0.0025 Concentrated iron solution (MS): FeSO4.7H2O (Ferrous sulfate) 27.30 2.73 Na2EDTA (Sodium EDTA) 37.30 3.37 Concentrated organic solution (MS): Myo-inositol 100.00 10 Glycine 2.00 0.2 Thiamine.HCl (Thiamine hydrochloride) 0.10 0.01 Pyridoxin.HCl (Pyridoxine hydrochloride) 0.05 0.05 Nicotinic acid 0.50 0.05 ______________________________________________________________________________________________________________________ Add 30.00 g/L of sugar and 8.00 g of agar, then boil until the agar dissolves. Add the growth regulator from the cytokinin group, namely BA (N6-benzyladenine), at a concentration of 3.00 mg/L, along with the hormone from the auxin group, namely NAA (alpha-Naphthalene acetic acid), at a concentration of 0.10 mg/L. This can induce the most shoots, 243 shoots, with new shoots appearing within 25 days after culture. A study of the culture medium formula for inducing complete plants from tissue culture of *Bauhinia acuminata* leaves found that the best formula is... The MS (Multimediated Biologics) nutrient formula supplemented with the cytokinin growth regulator BA (N6-benzyladenine) at a concentration of 3.00 mg/L, along with the auxin hormone NAA (Naphthalene acctic acid) at 0.10 mg/L, induced the highest number of shoots, specifically 243 shoots per initial plant explant within 25 days.

Claims (1)

1. The tissue culture medium formula for inducing the development of complete plants consists of MS medium supplemented with the growth regulator BA (N6-benzyl adenine) at a concentration of 3.00 mg/L combined with NAA (Naphthalene acctic acid) at 0.10 mg/L.