SU966567A1 - Mixing cell for conducting flow-through measurements of electronic paramagnetic resonance of liquid samples - Google Patents
Mixing cell for conducting flow-through measurements of electronic paramagnetic resonance of liquid samples Download PDFInfo
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- SU966567A1 SU966567A1 SU813229853A SU3229853A SU966567A1 SU 966567 A1 SU966567 A1 SU 966567A1 SU 813229853 A SU813229853 A SU 813229853A SU 3229853 A SU3229853 A SU 3229853A SU 966567 A1 SU966567 A1 SU 966567A1
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- SU
- USSR - Soviet Union
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- measurements
- liquid samples
- paramagnetic resonance
- mixing cell
- conducting flow
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Description
вании короткоживущих парамагнитных продуктов реакции, т. е. врем между началом реакции и измерением ЭПР (мертвое врем ) будет большим. Чтобы досгагнуть уменьшени последнего, необходимо увеличить скорость потока смеси, что требует большое количество реагентов и вл етс серьезным ограничением при работе с редкими биомолекулами или с ферментативными реакци ми. Кроме того, дл проведени измерений ЭПР химических и биохимических реакций при различных температурах, необходимо термостатировать всю систему целиком, что вызовет значительный расход теплоносител или помешать в термостат только смесительную камеру, а измерительную трубку тщательно теплоизолировать по всей ее длине. В последнем случае из-за большой прот женности измерительной трубки будеIn the case of short-lived paramagnetic reaction products, i.e., the time between the start of the reaction and the EPR (dead time) measurement will be large. In order to achieve a decrease in the latter, it is necessary to increase the flow rate of the mixture, which requires a large amount of reagents and is a serious limitation when working with rare biomolecules or with enzymatic reactions. In addition, in order to measure EPR chemical and biochemical reactions at different temperatures, it is necessary to thermostat the entire system, which will cause a significant flow of heat carrier or prevent only the mixing chamber in the thermostat, and the measuring tube should be carefully insulated along its entire length. In the latter case, due to the large length of the measuring tube,
создаватьс градиент температур по длине трубки, т. е. точность температурных измерений ЭПР будет невысока.create a temperature gradient along the length of the tube, i.e. the accuracy of the temperature measurements of the EPR will be low.
Цель изобретени - уменьшение габаритов чейки и повьпиение точности изме- 25 рений путем улучшени перемешивани измер емой жидкости и сокращени времени между началом реакции и измерением. Поставленна цель достигаетс тем, что в известную смесительную чейку дл проведени проточных измерений электрон- ного парамагнитного резонанса жидких образцов , состо щую из смесительной камеры , в верхней части квторой находитс пробка с отверсти ми дл подачи реагентов и инертного газа, измерительной трубки и мешалки, помещенной в смесительную камеру, дополнительно введена воронка, причем раструб воронки прикреплен внутри смесительной камеры в нижней ее части к внутренней стенке смесительной камеры в нескольких точках с зазором, ножка воронки расположена коаксиально в измерительной трубке, нижн часть измерительной трубки запа на, а верхн часть соединена непосредственно с дном смесительной камеры. Раструб воронки, укрепленный в нескол ких точках к стенкам смесительной камеры , имеет такую форму, чтобы между стен ками воронки и смесительной камеры существовал зазор, необходимый дл проте- каниУ1 жидкости. В смесительной камере помещаетс также мещалка, крыльчатка которой находитс над раструбом воронки, а ось мешалки проходит через пробку, закрывающую сверху смесительную камеру.:The purpose of the invention is to reduce the dimensions of the cell and to increase the accuracy of measurements by improving the mixing of the measured liquid and reducing the time between the start of the reaction and the measurement. This goal is achieved by the fact that in a well-known mixing cell for conducting flow measurements of the electron paramagnetic resonance of liquid samples, consisting of a mixing chamber, in the upper part of the second is a tube with reagent holes and an inert gas, measuring tube and stirrer placed a funnel is additionally inserted into the mixing chamber, and the funnel socket is attached inside the mixing chamber in its lower part to the inner wall of the mixing chamber at several points with a gap m funnel stem located coaxially in the measuring tube, the lower part of the measuring tube is sealed in, and the upper part is connected directly with the bottom of the mixing chamber. The funnel socket, fastened at several points to the walls of the mixing chamber, has such a shape that there is a gap between the walls of the funnel and the mixing chamber, which is necessary for the flow of liquid. A mixer is also placed in the mixing chamber, the impeller of which is located above the funnel mouth, and the axis of the agitator passes through the stopper covering the top of the mixing chamber .:
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU813229853A SU966567A1 (en) | 1981-01-05 | 1981-01-05 | Mixing cell for conducting flow-through measurements of electronic paramagnetic resonance of liquid samples |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU813229853A SU966567A1 (en) | 1981-01-05 | 1981-01-05 | Mixing cell for conducting flow-through measurements of electronic paramagnetic resonance of liquid samples |
Publications (1)
Publication Number | Publication Date |
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SU966567A1 true SU966567A1 (en) | 1982-10-15 |
Family
ID=20936411
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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SU813229853A SU966567A1 (en) | 1981-01-05 | 1981-01-05 | Mixing cell for conducting flow-through measurements of electronic paramagnetic resonance of liquid samples |
Country Status (1)
Country | Link |
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SU (1) | SU966567A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4769601A (en) * | 1985-08-22 | 1988-09-06 | Amoco Corporation | Method of and apparatus for determining cement strength and extent of setting with a pulsed nuclear magnetic resonance spectrometer |
-
1981
- 1981-01-05 SU SU813229853A patent/SU966567A1/en active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4769601A (en) * | 1985-08-22 | 1988-09-06 | Amoco Corporation | Method of and apparatus for determining cement strength and extent of setting with a pulsed nuclear magnetic resonance spectrometer |
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