SU961379A1 - Strain schwanniomyces alluvius-1167 as producer of alpha-galactoidase - Google Patents

Abstract

Schwanniomyces alluvius 1167 yeast strain (collection of cultures of industrial microorganisms of the Institute of All-Russia Research Institute of Genetics, collection number CMPM U-298), producing oi-galactosidase S. (L) Yeast cultures from the collection of the Institute of Microbiology of the Academy of Sciences of the USSR and the Department of Soil Biology were studied to obtain this strain soil science of Moscow State University named after MV Lomonosov As a result of research 05, a genus possessing .the ability to synthesize c6-galactosidase 00 Schvanniomyces was identified, and Schw alluvius - 1167 was recognized as the most promising strain. Katsii Laboratory of Enzyme Preparations of the Institute of Biochemistry iMoA.N.Bakha USSR Academy of Sciences under the number 1167 willows from the collection of the Institute of Scientific Research Institute of Genetics under the number of CMPM U-298o This strain has the following morphological and physiological characteristics: Schwannion ces alluvius, Phaff, Miller et Cooke Schwanniomyces alluvius-1 167 - isolated from sod-grass floodplain soil in Tula Ob.

Description

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Region, July 3, 1961 “The strain isolated has the following characteristics: Growth on the liquid side: after three days of cultivation at room temperature, the cells are round, up to oval (4.0-8.8) x (5.3-11.0), mainly in short chains or cells, weak ring and sediment.

Growth on wort - agar; after a month of cultivation, the stroke is light cream with a soft, glossy surface. The texture is pasty, weakly convex, the edge is even. Pseudomycelium is absent. Good sporulation after 5-7 days of growth on agar yeast autolysis with 2% glucose. Dispute 1-2 in asuka, round, warty, with a rim at the equator (like a walnut) o Fermentation: glucose (+), galactose (+), sucrose (+), maltose ("slow"), melibiosis (- or + very SLOW), raffinose (+), inulin (+) assimilation of carbohydrates: metabolizes glucose, galactose, b-sorbose, sucrose, maltose, cellobiose trehalose, melibiose, raffinose, melitsitovu, inulin, D-xylose, L-arabinose (weakly) B-Mannitol, B-glucitol, oymethyl, D-glucoside, Kraesmal solution, ethanol, glycerol, malic acid, citric acid (medpv).

Does not assimilate D-rabinose, inositol, lactose, L-rhamnose, erythritol, gapaktinol, lactic acid.

Nitrates and nitrites do not metabolize, It does not grow on non-vitamin medium. Gelatin does not thin

This medium provides a high level of biosynthesis - galactosidase in the culture fluid. Abroad, expensive peptone and meat extract are used in nutrient media for similar purposes.

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Example Seeding material served as a three-day yeast culture in the amount of 4%. The culture is grown at 28 ° C on a nutrient medium of the following composition, g / l:

Dry milk serum

85.6 (NH4) 2HP04 11.5 0.24

MnSO.

Yeast extract 0.3 Water distilled Else The medium is sterilized at 0.7 atm, 40 min.

Further submerged cultivation is carried out for 48 hours. The culture fluid is separated by centrifugation at 8c000 rpm for 10 minutes. The activity is determined using p-nitrophensh-ob-P-galactopyranoside as a substrate. The activity reaches 0.7 units / ml. The unit of activity was calculated per p-nitrophenol forming molecule at 37 ° С per 1 min

Thus, cultivation of the detected yeast strain Schwanniomyces allunius 1167 on a simpler and cheaper nutrient medium makes it possible to obtain highly active ei-gapaktozidazu 48 hours with weak invertase activity, which is involved in the breakdown of hard-to-digest carbohydrates, such as stachyose and 5 refinis, present in complex in composition of natural feeds; Preliminary treatment of soybean products with the preparation of β-galactosidase not only increases the use of soy protein, but also reduces the active gas Formation in the digestive tract, which is a disadvantage in the use of this type of feed.

Claims (1)

Thus, the cultivation of the identified yeast strain Zakkappgoshusez Punshshsh 1167 on a simpler and cheaper nutrient medium makes it possible to obtain highly active βί, -galactosidase for 48 hours with a weak invertase activity, which is involved in the breakdown of such difficult-to-digestible carbohydrates, such as stachiose and raffinose, that are present in decomposition, as well as in aging. composition of natural feeds. Pretreatment of soybean products with the “6-galactosidase” preparation not only increases the use of soy protein, but also reduces the active azoobrazovanie in the digestive tract, which is a disadvantage in the use of this type of feed.