SU955571A1 - Vaccine tf-130 (k) against trichophytosis of cattle and method for treatment and prophylaxis of trichophytosis of cattle - Google Patents

Abstract

1. Vaccine against cattle trichophytosis | first cattle, containing anti-en from living culture Trichophyton fariforme, protective environment including weelatin, sucrose and water, characterized in that, in order to increase immunogenicity, live culture Trichophyton fariforme 130 is used as an antigen. L when the concentration of microconidia of the specified culture of the fungus is 250-650 million, per 1 ml of protective medium of the following composition, May.%: Sucrose15-30 Gelatin2.0-8.0 Water Remaining 2. Vaccine according to claim 1, characterized in that it contains - antibiotics for 1 MP vaccine, units; Penicillin 50-100 Streptomycin 50-100 3. A method for the prevention and treatment of cattle trichophytosis, including intramuscular administration of an antigen preparation from a live culture of Trichophyton fariforme in the area of croup twice with an interval of 1014 days, characterized in that, in order to increase efficiency, as antigenic Trichophyton farifortne 130 L is used as a live culture, while for the prevention of young cattle under 5 months of age, the drug is administered in a dose of 1-2 ml, and older than 5 months in a dose of 2-4 ml. prepar Young cattle are introduced at an age of up to 5 months at a dose of 2-4 ml, and older than 5 months at a dose of 4-8 mp.

Description

The elimination relates to the field of veterinary mycology, in particular, to the vaccine against trichophytosis (shearbite) of bovine ckota,

The preparation TF-130 VIEV and the LTP-130 vaccine against cattle trichophytia are known, including Aytigen from live immunogenic culture Trichophyton fariforme strain 130, diluent - saline or distilled water, antibiotics - penicillin and streptomycin. LTP-130 includes an additional filler: sucrose, gelatin and water tl, 2

The drug TF-130 and the LTP-130 vaccine are administered intramuscularly in a dose of 5-20 ml, twice with an interval of 10-14 days.

The disadvantages of the drug TF-130 lj. are short shelf life (4 months) and hard temperature storage.

The disadvantages of the vaccine. LTP-130 2 is the administration of a large volume of immunization dose (5-20 ml with 4-10 million vaccine ready for use, viable microconidia) in 1 ml, the need to release in connection with this a large mass of sterilizer (per 1000 doses from 5 to 20 l) and a relatively short shelf life of 12 months.

The BLTP-130 p2 vaccine is taken as a prototype.

The purpose of the invention is to increase the immunogenicity of the vaccine by increasing the output of viable microconidia and reducing the volume of the injected dose.

To achieve this goal, it contains the Trichophyton fariforme antigen. 130 L when the concentration of microconium specified culture of the fungus 250650 million, 1 ml of protective medium of the following composition, wt.%:

Sucrose15-30

Gelatin2.0-8.0

WaterEverything

To achieve the goal, the vaccine also contains antibiotics per 1 ml of vaccine, units:

Penicillin 50-100 Streptomycin 50-100, To improve the effectiveness of a method for the prevention and treatment of trichophytosis involving intramuscular administration of an antigenic preparation from a live Trichophyton fariforme package in the croup region twice with an interval of 10-14

days, a living culture of Trichophyton fariforme 130 is used as an antigenic drug for the prevention of young cattle under 5 months of age; the drug is administered at a dose of 1 ml; and older than 5 months at a dose of 2-4 ml, and for treatment the drug is administered to young cattle under the age of 5 months at a dose of 2-4 ml, and older than 5 months at a dose of 4-8 ml.

Example. T. fariforme TF-130 was obtained in the process of long-term stepwise selection on the basis of sporulation and possesses blind properties.

Cultural and morphological properties.

Mycelium, colorless, septic, even, sometimes raciform from 2 to 5 MkM in diameter. Microconidium is oval, round or pear-shaped, 1-2 µm in diameter, 2-4 µm in length. Artrospores are rarely formed, have chains, and have a round or almost quadrangular shape. Chlamydospores specimens rarely usually in old culture overgrown. The resulting extremely rare micro conidia of a spindle-shaped form have 3-5 partitions, a diameter of 5-8 microns, a length of 30-50 microns.

The T.fariforme .TF-130L culture is infused on wort agar. For the preparation of wort agar, a beer non-boiled wort is used with an acidity of 1.6-2.4 (optimum 1.9), sugar content 6-10% (optimum 7%) according to Bapling, pH after sterilization 6.2-7.0 ( optimum 6.8).

The culture is grown at 25-30 ° C (optimum 26 ° C) for 10-15 (optimum 12) days, after which it is used to prepare a vaccine.

Wort agar. The colonies are white, fluffy, creamy, with a crater-shaped depression in the center, well-pronounced, multiple. Concentrated zonality, densely growing into agar, does not form a pigment.

Carrot-potato agar. Colony is flat, white, in the center with a yellowish tinge, aerial mycelium is developed moderately, the reverse side in the center is yellow.

Agar Grigoraki. The colonies are white, flat, on the periphery of the cobweb leaf, the back side is yellowish, there is a wide zone of clarification around the colony. Agar for Penicillium. Colony is white, flat, weakly folded in the center, aerial mycelium developed weakly growing cobwebby margin. Sporulation At the 10–15 th stage of the T.fariforme TF-130 L culture, on the wort agar, the concentration of microconidia in the homogenate was 350,650 million Ump, the output of the vaccine from 1 mat was 7–14 l. Antigenic properties. When animals are immunized, the formation of specific agglutinins occurs. The titer of the immunized bodies is RA 1: 80-1: 1280. Virulence. When applied to the scarified skin of an animal, a mild infection occurs at the place where the culture is applied, without forming a cap characteristic of the virulent strain of the hair; Self Recovery after 20-30 days. Secondary foci are not formed. Not dangerous for human infection when working with a strain; dangerous for animals in contact with the vaccinated, Reactogenicity. The introduction of a live culture to animals does not lead to the clinical manifestation of the disease, and isemenei in general condition: an increase in temperature at the site of administration, swelling, and overall body temperature. Immunogenic activity. T.fariforme 130 L cultures when applied to cattle for immunization creates a tense immunity. Immunity in animals 20-30 days after vaccine administration. The duration of immunity is not me-. It is 10 years old .. Before use, the vaccine is resuspended in sterile saline at the rate of 1 dose of vaccine per 1 ml of solvent, administered intramuscularly in the croup area at a dose of 1-2 MP for a prophylactic purpose and at a dose of 2-4 MP for a treatment. , twice with an interval of 10-14 days. The vaccine from this Strain with positive results was tested on 30,000,000 large cattle in the Union republics of the country.1. The vaccine provides for the prevention and treatment of cattle trichophytia. Due to the use of this culture in the vaccine, a 3-fold decrease in the raw material consumption for growing the crop, a 3-5-fold reduction in the production cycle, an increase in the vaccine output by 3-5 times, the viability of microcranidia increases by 3-5 times. The solvent release and the volume of glass containers are reduced 5 times. The use of the vaccine reduces the time spent on processing one animal, 5 times reduces the need for a solvent (1 dose of 1-4. MP), which reduces the cost of its manufacture and transportation. The use of this vaccine in veterinary practice will allow the elimination of cattle disease trichophytosis in the country.

Claims (3)

1. A vaccine against cattle trichophytosis containing antichine from the live culture of Trichophyton fariforme, a protective environment including gelatin, sucrose water, characterized in that, in order to increase immunogenicity, the living culture of Trichophyton fariforme 130 L is used as antigen at a concentration microconidium of the indicated culture of the fungus 250-650 million per 1 ml of protective medium of the following composition, May.% Sucrose 15-30 ' Gelatin 2.0-8.0 Water Else 2. The vaccine according to claim 1, characterized in that it contains antibiotics per 1 megapixel vaccine, unit: Penicillin 50-100 Streptomycin 50-100 3. A method for the prevention and treatment of cattle trichophytosis ^ involving intramuscular administration of an antigenic preparation from a live culture of Trichophyton fariforme in the area of § croup twice with an interval of 1014 days, characterized in that, in order to increase efficiency, Tricho live culture is used as an antigenic preparation - S phyton fariforme 130 L, while for “prophylaxis of young cattle under the age of 5 months, the drug is administered in a dose of 1-2 ml, and older than 5 months · <in a dose of 2-4 ml, and for treatment introduce young large ro atogo livestock before the age of 5 months in a dose of 2.4 ml, and over 5 months in a dose of 4-8 ml.