SU922632A1 - Device for micro-object scanning - Google Patents

Description

The invention relates to a microbeid: logic and medicine, in particular to a morphometric analysis of microobjects.

A device for mechanical scanning of microobjects is known, including a hexagonal rotating glass prism, a swinging mirror, a disk, a scan using a rotating disk with slots, an endlessly moving tape with holes and a scan using two co-axially rotating disks with spiral slits [1 ].

The specified device is difficult in design, commissioning, not reliable enough to work and does not provide measurement friction.

The closest in technical essence and the achieved result is a device for scanning microobjects, containing an optical device, a table for microobjects communicated with it, a reversible motor mounted on it to provide reciprocating movement and a photomultiplier, and analyzing devices, the information input of which is connected to the output of the photomultiplier [ 2].

This device provides scanning of an enlarged image due to the reversal of the engines and their movement of the diaphragm. When taking information about the geometric parameters of microobjects, e.g.

However, this device does not allow for high measurement accuracy and repeatability · due to the fact that when reversing the engines there is a section of braking and acceleration, i.e. scanning speed does not remain constant. To eliminate this drawback, complex mechanical 5 systems are used or the measurement field is narrowed relative to the scan line, which further complicates the device.

The purpose of the invention is improving the accuracy of measurements and ensuring their reproducibility.

This goal is achieved by the fact that a device for scanning Microobjects (containing an optical device 15 connected with (A table for microobjects, an inverted reverse motor for providing reciprocating movement and a photo-20 multiplier, and an analyzing device , the information input of which is connected to the output of the photomultiplier, contains a disk mounted on the axis of rotation of the engine with magnets evenly spaced along its periphery, a reed switch mounted on a table with the possibility of con Act c. one of the magnets and reed connected to a monostable multivibrator, whose output ₃₀ is connected to the resolving · entry analysis apparatus,

In FIG. 1 shows a device for scanning microobjects, front and top views; in FIG. 2 scheme of the device; in FIG. 3 "device in a perspective view.

The device contains an optical device 1, a stage 2 _connected with it for microobjects, 4 reversible motors mounted on the stage 2 for providing a return of translational motion, a photomultiplier 4 and a reed switch 5> an analyzing device 6 and a disk mounted on the axis of rotation of the motor. 3 7 with magnets 8 evenly spaced around its periphery.

The reed switch 5 is mounted on the table 2 with the possibility of contact with one of the magnets 8, while a waiting multivibrator 9 is connected to the reed switch 5, the output of which is connected to the resolving input of the analyzing device 6. The information input of the analyzing device is connected to the output of the photomultiplier 4.

The device operates as follows.

The light passing through the preparation in the optical device 1, falls on the diaphragm (not shown in the drawing) in the stage 2, is converted into a proportional photocurrent using a photomultiplier 4 and then goes on to the analyzing device 6.

The engine 3 is scanned by moving the stage 2 with the diaphragm relative to the enlarged image of the object. Magnets 8, alternately passing by the reed switch 5> cause it to operate and the associated waiting multivibrator 9, which gives a pulse to the resolving input of the analyzing device 6 of a given duration.

Thus, the measurement is carried out at equal intervals of distance in the enlarged image along the scan line, not depending on the speed of rotation of the engine 3.

The proposed device allows to increase the accuracy of measuring the geometric parameters of biological microobjects and to ensure high reproducibility of measurements. It is simple in design and reliable in operation. e

Claims (3)

() SCANNING DEVICE The invention relates to microbio; logies and medicine, in particular, to the morphometric analysis of microobjects. A device for mechanical scanning of micro-objects is known, comprising a hexagonal rotating glass prism, a swinging mirror, a disk, a scan using a rotating disc with slots, an infinitely moving tape with turns and a scan using two CO base-rotating discs with spiral 1J . The indicated device is difficult in its construction, adjustment, is not reliable enough in operation and does not provide measurement accuracy. The closest in technical essence and achieved result is a device for scanning microscopic objects, comprising an optical device, it reported -c table for microobjects ukMIKROOBEKTOV replennye thereon reversyvnyi vozvratnopostupatelnogo motor to provide movement and the photomultiplier, and analyzing device information input connected to the output photomultiplier 2. This device scans an enlarged image by reversing the motors and moving the diaphragm. When information on the geometrical parameters of microobjects, for example, is taken, the measurement is made at regular intervals, which at a constant scanning speed correspond to the same distances in the magnified image along the scanning line. However, this device does not allow to provide high accuracy of measurements and their repeatability due to the fact that when reversing 3 engines there is an area of braking and acceleration, i.e. scanning speed does not remain constant. To eliminate this drawback, complex mechanical systems are used or the measurement field is narrowed relative to the scan line, which further complicates the device. The purpose of the invention is to improve the accuracy of measurements and ensure their reproducibility. This goal is achieved by an NTO device for scanning Micro-objects, containing an optical device, a microproject table, a reversing motor mounted on it to provide reciprocating movement and a photomultiplier, and an analyzing device, the information input of which is connected to the output. The photomultiplier contains a disk mounted on the axis of the motor vortex with magnet uniformly located on its periphery, a reed switch mounted on a table with the possibility of contact with. one of the magnets, and a waiting multivibrator connected to the geok, the output of which is connected to the permitting input of the analyzing device. FIG. 1 shows a device for scanning micro-objects, front and top view; in fig. 2 device diagram; in fig. 3 device in axonometric view. The device contains an optical device 1, a table 2 for micro-objects connected with it, a reversing motor 3 mounted on a table 2 for providing a back-and-forth movement, a photomultiplier C and a reed switch 5 analyzing device 6 and a disk 7 mounted on the axis of rotation of the engine. with equally located magnets 8 around its periphery. The reed switch 5 is mounted on the table 2 with the possibility of contact with one of the magnets 8, while the waiting multivibrator 9 is connected to the reed switch 5, the output of which is connected to the enabling input analysis 6. An information input of the analyzing device is connected to the output of the photomultiplier k. The device works the next time. The light passing through the preparation in the optical device 1 hits the diaphragm (not shown) in table 2, is transformed into a proportional photocurrent using photomultiplier k and then goes to the analyzing device 6. Engine 3 performs scanning by moving table 2 with the diaphragm relative to the magnified image of the object. The magnets 8, alternately passing by the reed switch 5, trigger it and the associated multivibrator 9 associated with it, which gives a pulse to the enable input of the analyzing device 6 of a predetermined duration. Thus, the measurement is carried out at equal intervals of distance in a large image along a scanning line, independent of the speed of rotation of the engine. 3. The proposed device allows to increase the accuracy of measuring the geometrical parameters of biological micro-objects and to ensure trot reproducibility of measurements. It is simple in design and reliable in operation. Claims An apparatus for scanning micro-objects, comprising an optical device, a table for micro-objects communicating with it, a reversing motor mounted thereon for providing reciprocating movement and a photomultiplier, and an analyzing device, whose information input is connected to the photomultiplier output, characterized in that, in order to improve the accuracy of measurements and ensure their reproducibility, it is co. holds a drive mounted on an axis of rotation of an engine with magnets evenly spaced around its periphery, a reed switch mounted on a table with the possibility of contact with one of the magnets, and a waiting multivibrator connected to the reed switch, the output of which is connected to the enabling input of the analyzing device. Sources of information taken into account during the examination 1. USSR Author's Certificate No., cl. A 61 B 6/00, 1972. Avtandilov G.G. Morphometry in pathology. M., Medicine, 1973, with .. Fi & .2.