SU907418A1 - Method of diagnostics of septic condition of new born - Google Patents
Method of diagnostics of septic condition of new born Download PDFInfo
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- SU907418A1 SU907418A1 SU802940833A SU2940833A SU907418A1 SU 907418 A1 SU907418 A1 SU 907418A1 SU 802940833 A SU802940833 A SU 802940833A SU 2940833 A SU2940833 A SU 2940833A SU 907418 A1 SU907418 A1 SU 907418A1
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- septic
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- rna
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(54) СПОСОБ ДИЛгаОСТИКИ СЕПТИЧЕСКИХ состо ний у НОВОРОЖДЕННЫХ(54) METHOD OF DILGAOSTICS OF SEPTIC CONDITIONS IN NEWBORN
II
Изобретение относитс к медицине, в частности к способам диагностики внутренних заболеваний .This invention relates to medicine, in particular to methods for diagnosing internal diseases.
Известен способ диагностики септических состо ний у новорожденных, включающий определение количества лимфоцитов в периферической крови 1.A known method for the diagnosis of septic conditions in newborns, including determining the number of lymphocytes in peripheral blood 1.
Однако известный способ не обеспечивает высокой точности.However, the known method does not provide high accuracy.
Цель изобретени - повышение точности способа.The purpose of the invention is to improve the accuracy of the method.
Поставле1ша цель достигаетс тем, что при осуществлении способа диагностики септических состо ний у новорожденных, включающий определение количества лимфоцитов в периферической крови, в лимфоцитах определ ют содержание рибонуклеиновой кислоты и при ее показател х от 0,33 - до 0,99 диагностируют септический процесс.The goal is achieved by the fact that when a method of diagnosing septic conditions in newborns is carried out, including determining the number of lymphocytes in the peripheral blood, the content of ribonucleic acid in lymphocytes is determined and, if measured from 0.33, a septic process is diagnosed.
Способ осуществл ют следующим образом.The method is carried out as follows.
Из м коти пальца больного берут 1 каплю крови, нанос т мазок на предметное стекло . Мазок фиксируют. Мазок крови окрашивают реактивом Браше в течение 15 мин. ПодFrom a patient's finger, one drop of blood is taken, a smear is put on a glass slide. Smear fix. A blood smear is stained with Brashe reagent for 15 minutes. Under
иммерсионным микроскопом с увеличением X 450 подсчитьшают 100 лимфоцитов с разделением их на 4 степени концентрации рибонуклеиновой кислоты (РНК). Чистохимический показатель содержа1ш (ГПС) вывод т по формуле Асталди и Верга:With an increase in X 450 with an immersion microscope, 100 lymphocytes are calculated, dividing them into 4 degrees of ribonucleic acid (RNA) concentration. Chistochemical index containing 1x (HPS) is derived by the Astaldi and Verga formula:
За + 2в + 1с + о Over + 2c + 1c + o
ГПС 100GPS 100
где За - приведение количества лимфоцитов where For - bringing the number of lymphocytes
10 с 3 степенью концентрации РНК на их коэффициент; 2в - произведение количества лимфоцитов со 2 степенью концентрации РНК на их коэффициент 2 и т.д.10 with 3 degree of concentration of RNA on their ratio; 2c - the product of the number of lymphocytes with 2 degree of concentration of RNA by their coefficient 2, etc.
По сниже1шю чистохимического показате15 л содержани (ГПС) рибонуклеиновой кислоты (РНК) ниже 1,0 суд т о наличии септического процесса.By reducing the pure chemical index of ribonucleic acid (RNA) content (HPL) below 1.0, a septic process is judged.
При местных гнойно-воспалительных процессах у новорожденных детей, в частности When local purulent-inflammatory processes in newborns, in particular
20 при пиодерми х концентраци РНК существенно не отличаетс от показателей здоровых новорожденных - 1,35±0,13 (р 0,05) при норме 1,55±0,14. У больных с септическим20 with pyoderma, the concentration of RNA does not differ significantly from that of healthy newborns - 1.35 ± 0.13 (p 0.05) at a rate of 1.55 ± 0.14. In patients with septic
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процессом наблюдаетс резкое снижение концентрации №Ж в среднем до 0,99±0,06. Этот показатель достоверно отличаетс не только от ГПС РНК здоровых новорожденных (Р 0,001), но и ниже показателей больных имевших про вле1га пиодермии (р 0,01). По мере обратного развити клинических симптомов у больных пиодермией концентраци РНК и периоду рекогшалесценции нормализуетс , достига 1,41±0,07 (р 0,05). У боль- ю ных перенесших септический процесс, несмотр на клиническое выздоровление, нормализаци ГПС РНК не наступает - 1,28+0,05 (,05).the process shows a sharp decrease in the concentration of LH on average to 0.99 ± 0.06. This indicator is significantly different not only from the HPS RNA of healthy newborns (P 0.001), but also lower than those of patients who have suffered from pyoderma (p 0.01). As the clinical symptoms develop backward in patients with pyoderma, the concentration of RNA and the period of re-accumulation are normalized, reaching 1.41 ± 0.07 (p 0.05). In patients who underwent a septic process, despite clinical recovery, normalization of GPS RNA does not occur - 1.28 + 0.05 (, 05).
Предлагаемый способ диагностики септи- is ческих состо ний у новорожденных более чувствителен и информативен, не зависит от физиологических состо ний, позвол ет судить о тече1ши потологического процесса и приводить патогенетически обоснованную терапию, 20The proposed method for diagnosing septic conditions in newborns is more sensitive and informative, does not depend on physiological conditions, allows to judge about the flow of the pathological process and lead to pathogenetically based therapy.
что свидетельствует о повышении точности способа.which indicates an increase in the accuracy of the method.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU802940833A SU907418A1 (en) | 1980-06-13 | 1980-06-13 | Method of diagnostics of septic condition of new born |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU802940833A SU907418A1 (en) | 1980-06-13 | 1980-06-13 | Method of diagnostics of septic condition of new born |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU907418A1 true SU907418A1 (en) | 1982-02-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU802940833A SU907418A1 (en) | 1980-06-13 | 1980-06-13 | Method of diagnostics of septic condition of new born |
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| Country | Link |
|---|---|
| SU (1) | SU907418A1 (en) |
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1980
- 1980-06-13 SU SU802940833A patent/SU907418A1/en active
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