SU859929A1 - Method of determination of lithium in blood - Google Patents

Description

(54) CLUSTER FOR DETERMINATION OF LITHIUM IN THE BLOOD

Invention: relates to medicine and can be used in psychiatry to control the treatment of psychoses with salts, lithium. A known method for determining lithium is carried out by treating 2-3 ml of blood with a solution of trichloroacetic acid, followed by removal of proteins and quantitative analysis of lithium in a non-deposition fluid using flame photometry l. The disadvantage of this method is that it is very labor intensive, has a length of research and lacks accuracy. The purpose of the invention is to improve the accuracy and simplify the method. This goal is achieved by the fact that in the method of determining lithium in blood by obtaining serum with subsequent treatment and flame photometry, the treatment is carried out with distilled water from pH 6.9 to 7.0 to a final ratio of 1: 50-1: 60. Example. 0.4-0.5 ml of capillary blood is taken into a centrifuge tube and serum is obtained from it by centrifugation at 1500 rpm for 15-20 minutes. 0.1 ml of serum is mixed in another centrifuge tube with 4.9 ml of distilled water (dilution of serum 50 times). In the resulting sample, the luminosity in vivo is measured on a fiery photo no. The indicator of the intensity of the glow of the control solution with concentration, mln equivalent, is subtracted from the readings of the instrument: ml: 2.8; K 0.082; Ca 0.1. The final calculation of lithium content in serum is performed by calibrating a standard lithium solution. Calibration. The main standard solution with a concentration of 20 ml of lithium (739 ng of lithium carbonate is dissolved

38599294

in I l of distilled water), dilute the ammeter with a photometer 100 times with a control solution. metry test sample.

Of. This standard is prepared over a table. Figure 2 shows comparatively diluted dilutions that are processed and photometreated in the same way as an experimental sample.

Example. Construction of the calibration table. I

Table 1

Data of quantitative determination of lithium by known and proposed methods.

table 2

Claims (1)

Claim A method for determining lithium in the blood by obtaining blood serum, followed by processing it and flame photometry, characterized in that, in order to improve the accuracy of the method and simplify it, the treatment is carried out with distilled water with a pH of 6.9-. 7.0 to a final ratio of 1:50 1:60.