SU859929A1 - Method of determination of lithium in blood - Google Patents
Method of determination of lithium in blood Download PDFInfo
- Publication number
- SU859929A1 SU859929A1 SU802877503A SU2877503A SU859929A1 SU 859929 A1 SU859929 A1 SU 859929A1 SU 802877503 A SU802877503 A SU 802877503A SU 2877503 A SU2877503 A SU 2877503A SU 859929 A1 SU859929 A1 SU 859929A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- lithium
- blood
- determination
- serum
- distilled water
- Prior art date
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- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 title claims description 11
- 229910052744 lithium Inorganic materials 0.000 title claims description 11
- 238000000034 method Methods 0.000 title claims description 8
- 210000004369 blood Anatomy 0.000 title claims description 6
- 239000008280 blood Substances 0.000 title claims description 6
- 210000002966 serum Anatomy 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000005048 flame photometry Methods 0.000 claims description 3
- 239000000243 solution Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 208000028017 Psychotic disease Diseases 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- XGZVUEUWXADBQD-UHFFFAOYSA-L lithium carbonate Chemical compound [Li+].[Li+].[O-]C([O-])=O XGZVUEUWXADBQD-UHFFFAOYSA-L 0.000 description 1
- 229910052808 lithium carbonate Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Description
(54) С1ЮСОБ ОПРЕДЕЛЕНИЯ ЛИТИЯ В КРОВИ(54) CLUSTER FOR DETERMINATION OF LITHIUM IN THE BLOOD
Изобретение: относитс к медицине и может быть использовано в психиатрии дл контрол лече1щ психозов сол ми ., лити . Известен способ определени лити путем обработки 2-3 мл сьгаоротки крови раствором трихлоруксусной кислоты с последуюащм удалением белков и количественным анализом лити в иадосадочной жидкости методом пламенной фот метрии l . Недостататком этого способа вл ет с больша трудоемкость, длительность исследовани и недостаточна точность Цель изобретени - повышение точности и упрощение способа. Указанна цель достигаетс тем, что в способе определени лити в кро эи путем получени сыворотки крови с последующей обработкой ее и пламенной фотометрией, обработку ведут дистиллированной водой с рН 6,9-7,0 до конечного соотношени 1:50-1:60. Пример. Берут в центрифужную пробирку 0,4-0,5 мл капилл рной крови и получают из нее сыворотку путем центрифугировани при 1500 об/мин в течение 15-20 мин. 0,1 мл сыворотки смешивают в другой центрифужной пробирке с 4,9 мл дистиллированной воды (разведение сыворотки в 50 раз). В полученной пробе измер ют интвнсивность свечени на пламенном фото №тре . Из показани прибора вычитают соответствук ций показатель интеисивиости свечени контрольного раствора с концентрацией , м ллиэквивалент: На 2,8; К 0,082; Са 0,1. , Окончательный расчет содержани лити в сыворотке провод т по калибровке стандартного раствора лити . Калибровка. Основной стандартный раствор с концентрацией 20 мшшизквивалентов лити (739 нг углекислого лити раствор ютInvention: relates to medicine and can be used in psychiatry to control the treatment of psychoses with salts, lithium. A known method for determining lithium is carried out by treating 2-3 ml of blood with a solution of trichloroacetic acid, followed by removal of proteins and quantitative analysis of lithium in a non-deposition fluid using flame photometry l. The disadvantage of this method is that it is very labor intensive, has a length of research and lacks accuracy. The purpose of the invention is to improve the accuracy and simplify the method. This goal is achieved by the fact that in the method of determining lithium in blood by obtaining serum with subsequent treatment and flame photometry, the treatment is carried out with distilled water from pH 6.9 to 7.0 to a final ratio of 1: 50-1: 60. Example. 0.4-0.5 ml of capillary blood is taken into a centrifuge tube and serum is obtained from it by centrifugation at 1500 rpm for 15-20 minutes. 0.1 ml of serum is mixed in another centrifuge tube with 4.9 ml of distilled water (dilution of serum 50 times). In the resulting sample, the luminosity in vivo is measured on a fiery photo no. The indicator of the intensity of the glow of the control solution with concentration, mln equivalent, is subtracted from the readings of the instrument: ml: 2.8; K 0.082; Ca 0.1. The final calculation of lithium content in serum is performed by calibrating a standard lithium solution. Calibration. The main standard solution with a concentration of 20 ml of lithium (739 ng of lithium carbonate is dissolved
3859929438599294
в I л дистиллированной воды) раэбавл - ют на показани амперметра при фотоют в 100 раз контролымм раствором. метрии опытной пробы.in I l of distilled water), dilute the ammeter with a photometer 100 times with a control solution. metry test sample.
Из. этого стандарта готов т более В табл. 2 представлены сравнительдробнь№ разведени , которые обрабатывают и фотометрируют так же, как опытную пробу.Of. This standard is prepared over a table. Figure 2 shows comparatively diluted dilutions that are processed and photometreated in the same way as an experimental sample.
Пример. Построение калибровочной табл. IExample. Construction of the calibration table. I
Таблица 1Table 1
ные данные количественного определени лити известным и предлагаемым способами.Data of quantitative determination of lithium by known and proposed methods.
Таблица 2table 2
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU802877503A SU859929A1 (en) | 1980-02-13 | 1980-02-13 | Method of determination of lithium in blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU802877503A SU859929A1 (en) | 1980-02-13 | 1980-02-13 | Method of determination of lithium in blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU859929A1 true SU859929A1 (en) | 1981-08-30 |
Family
ID=20875525
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU802877503A SU859929A1 (en) | 1980-02-13 | 1980-02-13 | Method of determination of lithium in blood |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU859929A1 (en) |
-
1980
- 1980-02-13 SU SU802877503A patent/SU859929A1/en active
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