SU832140A1 - Apparatus for controlling liquid chromatograph - Google Patents

Description

(54) DEVICE FOR LIQUID CHROMATOGRAPH MANAGEMENT

The first valve 14, the output 11 of the first bones coon - with the drain, through the sixth valve 15, the output 8 of the second column 9, the inlet 5 of the first column 6, the adjustable throttle 16 and the hydraulic distributor 17, the first output channel 8 of which is connected to the control paddles of the first 2, second 7 and retego 10 valves, second output channel 19 with control cavities of the fourth 13, fifth 14 and sixth 15 valves, supply channel 20 with outlet 21 of pump 22 and through adjustable throttle 16 - with outlet

3 batchers 4.

The valve unit 1 has a housing 23, covers 24 and 25, membranes 26 and 27. A hydraulic accumulator 28 is connected to the outlet 21 of the pump 22. A command block 29 is connected to the hydraulic distributor 17.

Block 1 valves together with columns 6 and 9, if necessary, can be placed in a heated thermostat columns 30.

The device works as follows.

The element stream is supplied by a high pressure pump 22 through an adjustable choke 16 and a valve 2 to the column b. In the flow of eluent dispenser

4, the required amount of the mixture to be separated is supplied. From the column b, the eluent (eluate) is fed through valve 10 to column 9. From COLUMN 9, the eluent is directed through valve 7 to the outlet.

At the same time on the membrane 27 there is no pressure flow of eluent and valves 2, 7 and 10 are open. At this time, the membrane 26 is supplied with an eluent head), which

exceeds by a certain amount. eluent head in front of the columns, and valves 13,14 and 15 are closed. The difference between the flow rates of the eluents and is controlled by the throttle 16. When the beginning of the chromatographic front of an incompletely separated mixture appears at the exit of column 9, the flow of eluent is transmitted through the control unit 29 through the control valve 17 to the diaphragm 27 and removed from the membrane 26, thereby re-switching the interconnection of the elements of the elution system. Valves 13, 14 and 15 open and valves 2, 7 and 10 close. The flow of eluent is fed through valve 13 to column 9 and from column 9 through valve 15 to column 6. From column 6 through the valve 14, the flow of eluent (eluate) is directed to the output.

The rewiring is repeated with the multiplicity necessary to obtain the desired degree of separation of the injected samples. The volume of the diaphragm control diaphragm. Valves 2.7, 10, 13, 14, and 15 is so small that the switching of the latter by the flow of eluent during the separation is no interference

The eluent consumption for switching is completely insignificant. In addition, the spent eluent can be sent back to the eluent tank. If necessary, a hydraulic accumulator may be included in the route of the eluent to smooth out possible drops in the flow pressure during valve switching. The material of the membranes must withstand the necessary temperatures (up to 100 ° C), and the dimensions of the channels of the working cavity of the valves must be chosen so that the pressure of the membranes into the channels is prevented under high pressure. As a material for a membrane, for example, fluoroplast-4i can be successfully used.

The present invention allows to expand the range of use of high pressure recirculation liquid chromatography for the separation of substances, which are separated at a higher temperature and require rearrangement of the columns during separation, as well as significantly increase the reliability of the chromatograph with options of working with switching elements of the system eluting.

Claims (2)

Invention Formula A device for controlling a liquid chromatograph containing a block of valves, through the first of which the dispenser outlet is connected with the input of the first column, through the second valve the output of the second column — with a drain, through the third valve — the output of the first column — with the entrance of the second column, through the fourth valve the outlet of the dispenser with the inlet of the second column, through the fifth valve, the output of the first column with a drain, through the sixth valve the output of the second column with the entrance of the first column, characterized in that, in order to increase the reliability of the device The second throttle and hydraulic distributor, the first output channel of which is connected to the control cavities of the first, second and third valves, the second output channel - to the control cavities of the fourth, fifth and sixth valves / feed channel with the pump output and through an adjustable throttle - G outlet dispenser. Sources of information taken into account in the examination l.Parrrs N.A. Instrumental Liguid Chromatography. Elsevier Sicentific Publishing Company, Amsterdam, 1976, p.124. 2. USSR author's certificate No. 476503, cl. G 01 N 31/08, 1972 (prototype).