SU825615A1 - Culture medium for culturing microorganisms - Google Patents

Description

one

The invention relates to the microbiological industry, in particular to the cultivation of microorganisms producing protein biomass on hydrocarbon containing raw materials. .

Nutrient media containing sources of carbon, phosphorus, potassium and trace elements are known, while enrichment of tartaric acid ij is used to enrich the medium with microelements, or electrochemical decomposition of the metal is carried out directly in the medium when metal ions llj and 2 are introduced into it.

The disadvantages of the known methods include the fact that the use of production wastes is expensive due to transportation or complicated preparation of the nutrient medium.

The closest technical solution to the technical essence and the achieved effect to the proposed is a nutrient medium for growing microorganisms, containing sources of carbon, nitrogen, phosphorus, potassium, magnesium, iron, zinc, manganese ..

At the same time, a solution of mineral salts is first prepared by dissolving

2

in water, sources of nitrogen, phosphorus, potassium, magnesium, and trace element sources — ferrous sulphate, zinc sulphate and manganese sulphate — are introduced into a portion of this solution, previously acidified to pH 2.02.5, and then mixed with the remaining solution H.

The disadvantage of the nutrient medium is that ferrous sulfate is an expensive and scarce chemical, which limits its use on an industrial scale.

The purpose of the invention is to simplify with. preparation of the nutrient medium and its reduction in price with simultaneous utilization of titanium dioxide production.

This goal is achieved by the fact that the nutrient medium, including carbon, nitrogen, potassium / phosphorus, magnesium, iron, zinc, manganese and water sources, contains as a source of iron the production of two titanium oxide in the following ratio of components, g / l:

5.0 50.0

Carbon source

Production waste

0,008 1,5

titanium dioxide 1.0 8.0

Nitrogen source 4.0 8.0

Claims (3)

Source of phosphorus 0.2 6.5 Source of potassium 0.15 3.0 Source of magnesium 0.0005 0.5 Source of zinc 0.0003 0.15 Source of manganese Water Else Waste production of titanium dioxide is a mineral complex, which includes salt of ferrous sulfate - 85-95%, sulfuric acid - 0.8-1.5%, as well as impurities of manganese, zinc, copper, magnesium, cobalt, nickel, titanium. The impurities contained in the waste, in addition to ferrous sulfate, have a positive effect on the growth of microorganisms, and the presence of sulfuric acid provides an acidic reaction to the environment, which eliminates the need for additional acidification of the nutrient salt solution to prevent the formation of iron hydroxide and its precipitation. The proposed nutrient medium for growing microorganisms is prepared by mixing a carbon source with a solution of the mineral complex with waste from the production of titanium dioxide and the missing mineral salts of nitrogen, phosphorus, potassium, magnesium, zinc, manganese. Purified liquid paraffins, petroleum distillates, and alcohols can be used as carbon of the genus. Sulfuric salts of ammonium, magnesium, zinc, manganese, potassium sulfate or potassium chloride, ammophos are used as sources of mineral nutrition. The waste from the production of titanium dioxide is supplied to the nutrient medium either as a separately prepared solution or as a jointly prepared solution of potassium, zinc and manganese salts. Example. Nutrient medium for growing, yeast was prepared by mixing a source of carbon paraffins with an aqueous solution of the mineral complex — waste from the production of titanium dioxide, magnesium sulphate, potassium, nitrogen, zinc, manganese and ammophos in an amount, .g / L: n-paraffins with carbon atoms C-C (97% n-alkanes) .25.0 Waste from the production of titanium dioxide 0.15 Ammonium sulfate 1.0 Ammophos2.0 Magnesium sulfate 0.78 Potassium sulfate 1.60 Zinc sulfate0.06 Manganese sulfate 0.016 Candida Yeast qu.illierrnondii VSV-6.40 were grown in a continuous way in the apparatus with fur nical Move Shivani working volume of 12 liters at 32 dilution rate of 0.2 h iraskhod. air 1.8 l / min / l of medium, .рН 4, 2 was kept by adding ammonia to the medium. The biomass concentration in terms of absolutely dry yeast was 20 g / l. PRI me R 2. A nutrient medium for growing bacteria was prepared by mixing a carbon-methanol source with an aqueous solution of the mineral complex — waste from the production of titanium dioxide, sulfate salts of nitrogen, magnesium, potassium, zinc, manganese, and ammophos in an amount of g / l; Methanol 50,0 Waste from the production of titanium dioxide 1.5 Ammonium sulfate 8.0 Ammophos -8.0 Potassium sulfate 6, 5 Magnesium sulfate 3.0 Sulfate. Zinc 0.5 M manganese sulfate 0.15 Bacteria of the genus Mycococcus were grown periodically in Appa.-rate with mechanical stirring at 38 ° C,, 8, was maintained by adding ammonia to the medium. After 20h, the biomass growth was 35. g / l. PRI me R 3. Nutrient medium for growing yeast was prepared by mixing a carbon-n-paraffin source with an aqueous solution of the mineral complex — waste from the production of titanium dioxide and a solution of sulfate-ammonium, magnesium, zinc salts, manganese, potassium, and ammophos in an amount, g / l :, n-paraffins with the number of carbon atoms 22 Waste.production of titanium dioxide Ammonium sulfate Ammophos Potassium sulfate Magnesium sulfate 0.0005 Zinc sulfate 0.0003 Manganese sulfate Yeast Candida QiltiermondiiВСБ 542 was grown for 36 hours in flasks on a rocking pan with a volume of nutrient medium 100 ml at 32 ° C. Through . The 36 h biomass increase was 2.7 g / l in terms of absolutely dry yeast. Thus, the use of waste titanium dioxide production,. It allows the preparation of a nutrient medium for growing microorganisms without the expensive and scarce chemical of ferrous sulfate and simplifies the process of its preparation by eliminating sulfuric acid to acidify the medium in order to prevent the formation of iron hydroxide and precipitate it. Nutrient medium for growing microorganisms, containing sources of carbon, nitrogen, potassium, phosphorus, magnesium, iron, zinc, manganese, and water, so that, in order to simplify the preparation of the nutrient medium and reduce its cost, it contains as waste iron titanium dioxide in the following ratio of components, g / l: Carbon Source 5.0 .50.0 Production waste 0.003 1.5 titanium dioxide 1.0 8.0 Nitrogen source Source of phosphorus 0.4 8.0 Source of potassium 0.2 6.5 0.15 3.0 magnesium 0.0005 0.5 zinc 0.0003 0.15 manganese Else Sources of information taken into account in the examination certificate of the USSR 12 V 3/06, 1972. certificate of the USSR 12 V 3/00, 1969. certificate of the USSR 12 V 3/02, 1972