SU787995A1 - Method of differentiating t- and b-lymphocytes of a man - Google Patents

Description

The invention relates to biochemistry and can be used in clinical diagnostics. There is a known method for differentiating human T-and B-lymphocytes by treating them with a stimulator of the rosette formation, followed by staging the rosette reaction with mouse or sheep erythrocytes and evaluating the results of l. However, this method does not provide high accuracy. The aim of the invention is to improve the accuracy of the method. This goal is achieved by using 3- (2, .2,2-trimethylhydrazinium) propionate as a stimulator of the rosetting reaction. Example 1. Patient T. Dnagnosis is chronic lymphocytic leukemia with para protein Og M. The number of leukocytes is 33,000, of which lymphocytes are 81%. Before treatment with the preparation, the number of E sockets is 9%, the number of M sockets is 27%. After treatment with 3- (2,2,2-trimethylhydrazinium) propionate in a concentration of 0.2%, the number of E-sockets 20%, M-sockets 80%. Consequently, the patient T has chronic lymphole goats with abnormal V-lymphocytes. Example 2. Ill. Diagnosis - lymphogranulomatosis (nodular sclerotic form). The number of leukocytes 20,000, of which lymphocytes 17%. Before drug treatment, the number of E-sockets is 39%, M-sockets are 4%. After treatment of cells with a 0.0002% solution of 3- (2,2,2-trimethylhydrazine) propionate, the number of E-rosettes is 57%, M-rosettes are 29%. Thus, we managed to reveal the true correlation of lymphocyte populations. Example 3. Ill P. The diagnosis cannot be established:. The number of leukocytes 425,000 in mm, lymphocytes 99.6%. Before treatment with the preparation, the number of E-sockets is 4%, M-sockets. 15 %. This patient was diagnosed 2 years ago with chronic lymphocytic leukemia, about which the patient received a course of chemotherapy. It was reached full remission, continuing with 2 years. Before admission to the clinic, the patient's condition deteriorated dramatically. During the examination, it was established that the lymphoid cells of the peripheral blood of this patient morphologically differed significantly from the usual lymphocytes in chronic lymphocytic leukemia, they had a size of 15-20 microns, large 37,799 .54

Claims (1)

a bright light draw in which the formula of the invention is always there was one large, slightly basophilic honeycomb. These cells were more painful. The method of differentiation of T- and lupus resembled lymphoblasts than did small-lymphocytes in humans by treating lymphocytes, which are usually found as stimulators of the reaction of rozco-cochronic lymphocytic leukemia. The study with the subsequent formulation of reimmunological markers also did not repeat rosette formation with erythrocytes, and solved the question of the origin of mouse or ram evils and the assessment of the recurrent cells in this patient's results, and their immunological affiliation. 3- (2.2, of the method, as a stimulator of react2-trimethylhydrazine) with propionate in a rosace-formation, concentrations of 0.02% amount of E-ro-3- (2,2,2-trimetsh1hydrazinium) proprizo 4 are used. %, M-sockets 58%. The finalist is onat. ny diagnosis - regional crisis of chronic lymphocytic leukemia. Sources of information. The proposed method allows tSprints to be taken into account in the examination to accurately differentiate T-and B-lymphocytes in the peripheral blood of patients 1. Guidelines for immunology, lympho-proliferative diseases. Ed. In the call, 1973, p. 350-351.