SU729246A1 - Beaweria bassiana (bals) vuill 124-p strain for producing entomopathogenic beawerin preparate - Google Patents

Description

The invention relates to agricultural microbiology and can be used in the production of microbiological plant protection products. For the preparation of entomopathogenic paraverine boverine, the strain 113H-278 i is currently used. However, such a strain has a high productivity and virulence only when cultured in nutrient media containing expensive scarce raw material peptone and sucrose. When replacing peptone and sucrose with cheap industrial raw materials - dairy whey, corn extract, yeast casein and others, the culture, due to low physiological activity, uses all the components of the medium and instead of konyiospores forms gonidia and spore blast, rapidly losing their activity. The conditions on the regulatory environment are only 300 million spores per 1 m of culture liquid, the output is 4 g with 1 l of culture liquid and the virulence in LK is 0.02-0.002G. The duration of cultivation at an optimum temperature of 27 ° C is 72 hours. The aim of the invention is to provide the B-bassiano with the requirements of production and with higher physiological activity, productivity, virulence, as well as a shorter developmental cycle. The strain has the following characteristics. Morphological features On agar medium, Saburo forms round colonies, flat, pressed to the substrate, with a roller on the edge, without secondary growth and coremia. Conidial layer of chalky consistency. Pigmentation of the conidial layer of cream, pigmentation of the substrate is reddish. Limeter of 7 anneal cologia 10.1 i 0.08 mm.

L1Y .. surface koiyitospor 3,2 ± 0,01 microns.

On the agar medium of čapek, the colonies are round with blurred edges. Conidial layer wrinkled in center. The lower part of the colony has a radial folding, the riifTeness of the PII of the coniferous layer is from chalky white to ash-gray. On the medium, wort agar forms flat round colonies with a roller along the rim and a convex middle part. The lower part of the colony is red. Conidial layer of white and cream color. ,

Under deep conditions in a nutrient medium containing,%: sugar or whey powder 1.0, NOYO-0.9, DgbO 0.075, - 0.225, CaClg, -1.25, conidiospores are developed by one or two germ tubes. The bulk of the spores germinate after 12 hours. During 36-48 hours, the mycelium intensively develops, gonide splits off and and again / germinates. Conidiophores of the bottle-shaped form with an expanded base simultaneously form on the mycelium. Conidiospores are located on conidiosis, basipetally, on short sterigmas. Completion of sporulation and lysis of the mycelium occurs after 62 hours. The diameter of the deep conidiospores 3-5 microns.

Physiological signs. Attitude to carbohydrates. Well absorbs sucrose, glucose, lactose, maltose, arabinose, rhamnose, fructose. Poorly developed on xylose. Does not absorb cellulose. Starch intensively hydrolyzes.

Relation to nitrogen sources. Well absorbs albumin, casein. Intensively developed on peptone. Free amino acids stimulate conidia formation at a concentration of 15, mg% in the medium. At a concentration of from 20 to

50 mg% conidia is disturbed, gonidia and blastospores are formed. Well absorbs ammonium nitrogen.

(, (, NH4) HPO, “IDN ,,

and nitrates (NQMOg).

Relation to organic acids. Absorbs pyruvic, acetic, oxalic acid. Does not assimilate Hthifj acid.

Attitude to alcohols. Absorbs mannitol, dulcite, sorbitol, glycerin. Poorly developed on xylitol. Does not assimilate ethanol, isopropanol, n-butanol. The optimum cultivation temperature is 25-27 C. Aeration intensity is O, 5-1, O g 0 / p / h,

The proposed strain obtained by the method of selenium on the basis of monospore sieving culture /B.bQssiQna after passage through the Colorado potato beetle. The initial strain was obtained from the Institute of Biology of the Academy of Sciences of the Latvian SSR, Since the initial strain in deep conditions formed only the thidium and blastospore,. the study of the basic properties of the new strain is carried out in comparison with the proto-strain 113H-278, which is currently used in the microbiological industry to obtain deep conicospores.

The table shows the data on the productivity and virulence of the proposed and known strains in liquid nutrient media - in the regulatory series No. 1, with sugar), and in medium containing whey instead of sugar (medium No. 2).

As can be seen from the data in the table, the proposed strain is 1.5–2.0 times higher than that known for the biomass yield and titer of conidiospores, and for virulence 1O times. In this case, the dispute forchting of the proposed strain takes place in more than 5 short time (10 hours faster).

Claims (1)

72 0.29 ± 0.037 113Н-278 72 0.3910.120 Р-- Q-05 3.9 0, О0500 4.3 0.00210 Formula of isobrinum BassSuna strain (Loi Vn, intended for the production of an entomopathogenic preparation bovternna. Stored in Collections of the All-Union Scientific Research Institute of Microbiological Plant Protection Products and Bacterial Preparations. Morphological Features On the agar medium, Saburo forms colonies of round, flat, pressed to the substrate, with a roller on the edge, without secondary growth and coreia. Conidnal layer of cretaceous consistency. cream layer eta, pigmentation of the substrate is reddish.The diameter of 7-day colonies is 10, CO, 08 m The diameter of the surface conidiospores is 3.2i 0.01 μm. On the апapek agar medium, the colonies are round with diffuse edges. The conidial layer is flushed, towards the center. The lower part of the colony has radial folding. Pigmentation of the conidial layer from a creamy white to ash-gray color. In the medium, wort agar forms flat round colonies with a roller along the rim and a convex middle part. The lower part of the colony is red. The final layer is white and cream color. Under deep conditions in a nutrient medium containing sugar or milk powder 1.0%, NaHOj O,. 0.07 TU 1L oo / - iH D) g-p / 0.225%, CaCt /, 1.25%, conidiospores germinate with one or two germ tubes. The bulk of the spores germinate in 12 hours. Within 36-48 hours, the mycelium develops intensively, gonidia are released, and germinate again. At the same time, a conidiotic bottle-shaped form with an expanded base is formed on the mycelium. Conidiospores are located on conidiophores basipetally on short sterigms. Completion of sporulation and lysis of the mycelium occurs after 62 hours. The diameter of the deep end of the spores is 3-5 microns. Physiological signs Attitude to carbohydrates. Well absorbs sucrose, glucose, lactose, maltose, arabinose, rhamnose, faktoza. Starch intensively hydrolyzes. Poorly developed on xylose. Does not absorb Oellulose. Relation to nitrogen sources. Good ycBaHBieT albumin, casein. Intensively developed on peptone. Free amino acids stimulate conidium formation at a concentration of 15 mg% in the medium. At a concentration of from 20 to 50 mg% conidium formation is disturbed, gonidia and blastospores are formed. Well absorbs ammonium nitrogen and nitrates. Relation to organic acids. Absorbs pyruvic, acetic, oxalic acid. Does not absorb succinic acid. Attitude to alcohols. Absorbs lures, dulcite, sorbitol, glycert. Poorly developed on xylitol. Does not digest ethanol, isopropanol, n-butanol. The optimum cultivation temperature is 25-27 C. The intensity of aeration is 0.5-1.0 g Ol / l / h. Sources of information taken into account during the examination 1. USSR author's certificate 507645, cl. From 12 to 1/06, 1975 (prototype).