SU715996A1 - Inducer of eluate flow in chromatographic columns - Google Patents

Description

(54) EYUANT FLOW STROKE IN CHROMATOGRAPHIC

COLUMNS

strong resistance due to its rigidity. When the shell wall thickness is reduced, it becomes permeable to gas, which dissolves in the eluant, and then released in the chromatographic column, which ultimately leads to a decrease in the accuracy of the flow of the eluant.

The aim of the invention is to increase the flow rate stability and eliminate the gas saturation of the eluant.

The goal is achieved by the fact that, in a pneumatic inducer of an eluent flow of chromatographic columns consisting of a tank separated by an elastic sheath of a chemically resistant material and a pressure stabilizer, two elastic shells are additionally installed in the tank, one of which is made of dense material, such as rubber and a friend is made of a porous material, for example a fabric, all the shells are made in ly, and the sheath of a porous material is located between the two other membranes of other shells and is adjacent to them superficially station, and along the perimeter communicated with the atmosphere.

The drawing schematically shows a pneumatic flow booster consisting of reservoir 1, three separate shells 2, 3 and 4 of chemically resistant, porous, of dense materials, respectively, dividing the reservoir into cavities A and B, and communicating with the atmosphere at the perimeters 5, pressure stabilizer b and crane 7.

Pneumatic booster flow works as follows.

Eluant is charged through valve 7 into cavity B of tank 1. Gas from cavity A is expelled into the atmosphere. The pressure stabilizer 6 is filled. After filling cavity B, valve 7 is closed and compressed gas is dropped into cavity A through pressure stabilizer B. Compressed gas through the shells 2, 3 and 4 puts pressure on the eluant, which without pulsation enters the chromatographic column. At the same time, the compressed gas diffuses through the shell 4 of dense material and, having entered the shell 3 of porous material, enters the atmosphere around the perimeter 5. As the cavity is being emptied, cavity B is refilled with eluant.

Installing in the tank additionally two separation shells, making one of them of a porous material, and the second of dense material, installing a porous shell between the other two, with the possibility of snug fit, and also connecting it along the perimeter with the atmosphere allowed to transmit static pressure gas from a dense shell to a chemically resistant shell, eliminating the mass transfer of gas from the gas cavity of the tank into the cavity with the eluant, and the execution of the shells in the molds eliminated the effect of stiffness (pressure resistance gas) shells, thereby increasing the stability of the flow of eluant at the stream.

Claims (3)

1.The eluant flow stream in chromatographic columns containing a reservoir divided by an elastic sheath of chemically resistant material into two parts, one of which has a channel for supplying compressed gas with a pressure regulator installed in it, and the other part is connected to the source of eluant via a channel with a controlled valve and with a chromatographic column, characterized in that, in order to increase the stability of the flow rate of the eluant and eliminate its gas saturation, two elastic pipes are additionally installed in the tank s shell, one of which is made of a dense gas-tight material and the other - of a porous material, wherein the shell of porous material located between two drgimi, adjacent their surfaces and perimeter communicates with atme sphere, 2. Actor according to claim 1, characterized in that the shells are made in scoops. Sources of information taken into account in the examination 1. US Patent 141-234, No. 3765461, 1972. 2.naTefjT Japan CL. 63/3 / EN, № 50-139611 3. Devices for chromatography, M., Engineering 1973, p. 232. On ttpo amatfia uvteifif ttifaoHKi /