SU690066A1 - Cultivator for incubating microorganisms on porous film - Google Patents

Description

. one

The invention relates to devices for the surface cultivation of pure bacterial cultures, in particular in the manufacture of vaccines, and can be used in microbiology, epidemiology and medicine.

A cultivator for growing microorganisms on a porous film is known, comprising a hermetic body 1}.

In this cultivator there are horizontally arranged rings, between which a porous film and a plate are installed, while a liquid nutrient is placed under the film, and microorganisms are grown on the porous film.

The disadvantage of the known cultivator is the presence of a large number of rings with a large diameter and numerous tubular connections to collectors located outside the outside of the sealed enclosure, as a result of which sterility cannot be ensured, in particular, with continuous growth of microorganisms in production environments.

A disadvantage of the known cultivator is also the presence of a hydraulic column of a liquid nutrient medium, which leads to a rupture of the porous film, and excessive penetration of the nutrient medium through the porous film, leading to a deterioration in crop growth, and thus low productivity.

Known cultivator can be used for laboratory research and work.

The purpose of the invention is to ensure the continuity of the process, its sterility and increase productivity.

five

This is achieved by the fact that the proposed cultivator for growing microorganisms on a porous film contains a frame placed in a sealed enclosure on which a vertically mounted row of individual chambers of porous film for a nutrient medium, equipped with fittings for supplying and discharging the latter and wicker elements located inside them, are mounted distribution of nutrient medium. The chambers are separated from one another at the top and bottom by separating plates and form between themselves microorganism growth chambers,

0 in which movable elements are installed to remove cultivated culture and bi, and in the upper separator sprays the sprayers are fixed, and the lower ones have a channel for removal of the spent nutrient medium.

In addition, the cultivator is provided with a nutrient circulation system consisting of pipelines, heat exchangers, a sterilizing filter and a membrane pump.

At the same time in the tight case. in its lower part, a collection for the culture was placed.

FIG. Figures 1 and 2 are a schematic view of a cultivator for the growth of microorganisms, side view and sectional view; in fig. 3 - section nutrient medium chamber; in fig. 4-g perforated plate; in fig. 5 - insert For supplying the nutrient medium to the wick elements in the section; in fig. b and 7 - section a-i and bb of fig. five; in fig. 8 - sectional growth and feeding chamber of microorganisms; in fig. 9 - Moving elements to remove grown. culture; in fig. 10 - section bb firm. 9; in fig. 11 - sectional splitter plate; 12,13,14 - sections GG, D-D and EE of FIG. eleven; in fig. 15 is a separator plate with channels for removal of the spent nutrient medium; in fig. 16, 17 - sections Ж-Ж and К-К of FIG. 15.,.

The cultivator (Fig. 1, 2) contains a hermetic housing 1 with a cover 2 and a jacket 3, heaters 4; grandchild) in a frame with a frame consisting of vertical posts 5, horizontally longitudinal pipes 7, 8 and 9 and transverse posts (not shown), traverses 10 and 11, screws 12 and screw sliders with clips 13.

On the frame there are vertically mounted separate individual chambers of porous film for the nutrient medium, having nozzles 14, 15 and 16 for the supply and removal of the latter and outlet pipes 17 and 18.

The chamber (Fig. 3) for the nutrient medium consists of a perforated; plate 19, wick elements 20 and adjacent porous film 21 having a closed perimeter.

The perforated plate 19 (Fig. 4) has openings 22 arranged in a checkerboard pattern. Bottom, tube 23 is attached to the plate 19, and at the top it has a hanger 24.

On the pipes 7 there are hooks 25 of the inserts 26 (FIGS. 5-7) placed in the upper part of the cultivator and having channels 27 for supplying the nutrient medium to the wick elements. The channels 27 have through holes 28 of small diameter and fittings 29. At the bottom of the ends of the insert 26 there are suspensions 30.

The chambers for the nutrient medium are separated from one another at the top and bottom by separator plates 31 and 32 and form chambers between them.

33 growths of microorganisms (Fig. 8), in which mobile are mounted

elements for removing the dilated culture (Fig. 9.10).

Plate 31 (Fig. 11-14) mounted on the pipes 8 of the frame. At the ends, each plate 31 has notches 34, and inside it there are two channels 35 and 36 with fittings 37 and 38. On the bottom side of the Plate 31, the sprayers 39, having nozzles 40, a pipe 41 with a flange 42 and a gasket 43. At the bottom of the plate 31, holes 44 in the form of a semicircle are made on both sides at an equal distance.

Plates 32 (Figs. 15-17) have channels 45 for removal of the spent nutrient medium, holes 46 in the form of a semicircle on both sides, and central through holes 47. On the sides of the plate 32 Have an Insulation Strip 48, and on top - a chute 49.

The movable element for removing grown Culture consists of a plate 50 made of elastic material with edges in the form of petals 51, planks 52, 53 and a clip 54 (Fig. 9.10). .

The cultivator (Fig. 1, 2) also contains a nutrient medium circulation system consisting of pipelines 55-62, heat exchangers 63 and 64, a membrane pump 65 and a sterilizing filter 66..

In the lid 2 of the hermetic case 1 there are observation windows 67, and a collector 68 for the grown culture is placed along the frame.

The cultivator drive includes a reversing motor 69, a chain drive 70 with sprockets 71 and worm wheels 72.

The feed chambers for the nutrient medium and the microorganism growth chambers 33 are assembled on a frame outside the housing 1. On the hangers 30 of the inserts 26, the hangers 24 place a perforated plate 19 on which the wick elements 20 are hung. Then, the porous film 21 and the insert are mounted on the insert 26 26 hooks 25 hang on the pipe 7, and on the pipe 8 set cutouts

The separation plate 31, a succession of them, and then by means of cross-arms 10, 11 and nuts, these plates, seal the bottom of the porous films 21 and the tubes 23 with bolts clamped by the plates 32. At the bottom, a collection 68 for the grown culture is suspended on the pipes 9. Then the fittings are connected to the pipes 14, 15 and 16, respectively.

29, 37 and 38. In the growth chambers 33, mobile elements are inserted to remove the grown culture, whose ends are enclosed in cages 54, which are fixed at the ends of the extreme mobile elements.

Next, the frame is placed in the housing 1 so that the holder 54 entered the holder 13 of the screw slide. Then the pipes 14, 15 and 16 and the outlet pipes 17, 18 with flare nuts are connected respectively to the pipes 55-59 and the housing 1 is closed with the cover 2.

The cultivator works as follows

First, the cultivator is sterilized with primary steam, previously cleaned of mechanical and other impurities. Steam is supplied to the upper part of the housing 1 through pipe 60 (Fig. 1), and air is removed through pipe 61 below. Thereafter, the pipelines overlap and condensate is removed through line 62.

Cultivator sterilization is carried out for 30 minutes. Sterilization is carried out simultaneously with connected units and fittings. After sterilization is completed, the cultivator is cooled by supplying cold water to the jacket of the housing 3 to a thermostatic temperature. Having set the thermostat temperature, turn on the automatic equipment for maintaining it by means of the heaters 4. Then a sterile nutrient medium having a temperature equal to the thermostat temperature is supplied through the pipe 55 and the socket 14.

The supply of the nutrient medium is carried out under a slight pressure by pulses regulated by a time relay (not shown). The nutrient medium is injected through the holes 28 to the wicker elements 20, where it slides straight down and to the sides.

For seeding culture surfaces of porous films 21, a vessel with a suspension of seed culture is sterilely connected to pipeline 56 (through a sowing valve) connected to branch pipe 15, and installed at the level of separation plates 31. Then, a sterile air pressure of 0 , 3 atm through pipe 57 and pipe 16. Air enters simultaneously into all channels, and from there through nozzles carries seed material out of channels 35-, spraying it, into growth chamber 33, wetting the surface of porous materials. films 21. Simultaneously incorporated reversible electric motor 69 which through sprocket 71, chain drive 70, worm gear 72, screws 18 and screw sliders with yokes 13 drives the movable elements to remove the grown culture automatically. For seeding with a crop, it is sufficient to do this 2-3 times. After sowing 5 is completed, motor 65 is turned off,

the air is supplied to the pipe 16, the vessel with the seed is disconnected and the cultivator is left for the period of incubation. To create the necessary Q conditions for the growth of a culture, sterile air or another gas is supplied to housing 1.

During the incubation period, the nutrient medium is continuously supplied and continuously withdrawn: by the plates 32 through you. water pipe 18.

At the end of the incubation period, the grown culture is washed away with a saline solution or medium.

0 drying, for which the vessel with the: flush material is sterilely connected to the pipeline 56 connected to the pipe 15 and placed at the level of the separation plates 31. Then the same operations are repeated as in the seeding culture, feeding the flush material to the full removing the grown culture from the surfaces of the porous films 21. This. visually controlled through viewing windows 67.

When the washout is completed, the vessel is disconnected from the pipe 56. The washing culture suspension is collected in a collection container. 68, from where it is removed through the pipe 17 and the pipe 58, the vessel to which the culture suspension is transferred is sterilely connected to the latter.

After washing, a culture remains on the surfaces of the porous films 21,

0 therefore, the cultivator is left for re-cultivation. Such re-growths can continue until the biological properties of the culture change or until sterility is compromised.

5 The nutrient medium, going from top to bottom and also removed from housing 1, is then sent to heat exchanger 63, where it is heated to 85 ° C with an exposure time of 3-5 minutes to destroy exchange products, is passed through sterilizing filter 66 by means of a membrane pump 65 and a cooling heat exchanger 64 and further to the receiver - (not shown), and from it

5 is fed back to housing 1.

Porous films 21 can be used with pores from 0.15-0.5 mm, for example, parchment paper, glycerin cellophane, and the like.

 The leaks that occur inside housing 1 do not affect the process flow rate.

In order not to be easy with the new installation of cameras, the cultivator

5 must be equipped with a second set of movable frame, plates, nozzles.

The proposed cultivator for growing microorganisms on a porous film in comparison with the known one ensures the continuity of the process, its sterility and increased productivity and can be used in industrial production.

The proposed cultivator will allow the cultivation of microorganisms that are grown by the surface method, to obtain culture suspensions without the presence of a nutrient medium and metabolic products, which will increase the quality of the bacterium preparation. This is especially for crops that grow into the surface of a dense nutrient medium, such as fungus-ringworm (preparation TF 140).

Claims (3)

1. A cultivator for growing microorganisms on a porous film containing a sealed enclosure, for the reason that, in order to ensure the continuity of the process, its sterility and increase productivity, it contains a frame placed in a sealed enclosure. on which vertically mounted a series of individual chambers of a porous film for a nutrient medium, equipped with branch pipes for supplying and discharging the latter and wicker elements arranged inside them to distribute the nutrient medium, while the chambers are separated one from the other at the top and bottom by dividing plates and form cameras between them the growth of microorganisms, in which mobile elements are installed to remove the grown culture, and sprayers are fixed in the upper separation plates, and the lower ones have a channel Exhaust l culture medium. 2. Cultivator pop, 1, characterized in that it is equipped with a nutrient recirculation system consisting of pipelines, heat exchangers, a sterilizing filter and a membrane pump. 3. A cultivator according to claim 1, characterized in that a collection for the culture grown is placed in a hermetic case in its lower part along the frame. Information sources, . taken into account in the examination 1. USSR author's certificate 24543, cl. From 12 to 1/10, 1931. S3 70 7/72 to 7 a 2SI, 15 16 1 / .III / I I I I 3 I 72 7 / ($ 15 p 56 S7 70 2S 7 7f 59 63 ABOUT 2t to 19 / / / J2 ШЯЛ.Ш.ШШЖ1.Ш.1Ь talshshellllg r-t-o-i -jH 690066 15 s year  p ha I 6th A-A 6 Z6 FIG. eight IG.9 74 gd -e .F “& M Fa / h F 7