SU664639A1 - Method of determining the parameters of blood erythrocytometric curve - Google Patents

Description

one

This invention relates to the field of medicine, namely to hematology.

There is a method for determining the parameters of the erythrocytometric blood curve by measuring the optical density spectrum of a diluted blood sample in a certain wavelength range and then calculating the obtained values of the parameters of the erythrocytometric blood curve, erythrocyte radius and dispersion of the erythrocytometric curve.

However, the known method does not allow defining quickly and accurately.

The aim of the invention is to accelerate the improvement of the accuracy of the method.

This goal is achieved by measuring in a wavelength range of 0.5-1.0 µm.

FIG. 1 shows the dependence of the parameter of dispersion 1 on the value of aINl, where a is the value of the derivative with I, I, o, A, is the wavelength. L - diluted blood red blood cell concentration, / - cuvette thickness; in fig. Figure 2 shows a plot of go versus io, used to determine the most likely radius from the known .i and Ho - modal particle radius; in fig. 3 shows the spectra of derivatives of optical densities by wavelength, measured in spectral

interval 0.5-1 μm for three blood samples.

Example 1. Blood diluted 2000 times in a 2.3% NaCl solution is poured into a flat cell 1 cm thick and illuminated with a collimated beam of light (angle of divergence and source 27yst 30) of a monochromator with a dispersion of 50 A ° / mm measured are the spectrum of the derivative

optical density wavelength

Oa.

With a reception angle of 2up 27ist in the wavelength range of 0.50-1.00 µm, the maximum wavelength Yo is determined, at which

 a (in the range of 0,

d

0.8 microns, completely overlapping the possible sizes of red blood cells, 4-3.6 microns). In the area I, oh is found

 a const 2-16TOK, in which

ok

103 cm – 1 (0.85–1.00 µm), calculate the value of ajNl, where N is the concentration of red blood cells in diluted blood (L 2.0–3.5 10 cm –2), / is the thickness of the cuvette (cm). According to a known value, 5-3.5-10 cm of FIG. 1 determine the dispersion parameter | l in the range of possible values (5-

Claims (2)

15). According to Lo, x and FIG. 2 determines the most likely red blood cell radius. For example, if Yao is 0.70 μm, N 2.5106 cm-0 0 5.3-103 cm-1, cm, a / Nl 2.12-10-3 cm, then q. 8 th and 3.1 microns. The proposed method is carried out under single scatter conditions, therefore, dilution of whole blood is necessary. As a diluent, in addition to the above 2.3% NaCl solution, you can use 0.9% NaCl solution and blood plasma. In this case, the method of measuring Go and | remains the same, but the calibration curves change. For the implementation of the method, it is convenient to use the small angle version of the Unicum SP-700 spectrophotometer, equipped with an attachment, which makes it possible to measure directly the derivative of the optical density over the wavelength. Example 2. The value of a / Nl, where L is the concentration of erythrocytes in diluted blood (L 2.0-3.5-106 cm-s), is calculated from the value of const in the region I, I, o (0.85-I micron), / - the thickness of the cuvette (/ 1 cm). The dispersion parameter, M, is determined from FIG. 1 according to the calculated value a /. Further, according to the well-known AO, and and FIG. 2 are the most likely radius of Go. For example, for samples whose spectra of derivatives are shown in FIG. 3, there is the following table of the results of determining T0 and | 1. The use of the proposed method allows the rapid determination of the most important parameters of the erythrocytometric curve due to the reduction in the measurement volume (no measurement of the absorption index is required) and a reduction in the processing time. With this method, the time required for determining the desired parameters is reduced by 5-6 times in comparison with the prototype and is 10-15 minutes. The use of spectral equipment with dispersion A ° / mm in the above spectral range gives an error of 4o / o; ten%. The method is simple from the point of view of technical implementation and can be used in clinical laboratory analysis. The method of determining the parameters of an erythrocytometric blood curve by measuring the optical density spectrum of a diluted blood sample in a certain wavelength range and then calculating the obtained erythrocytometric blood parameters, the erythrocyte radius and the dispersion of an erythrocytometric curve, which is so that it accelerates and improves the accuracy of the method, measurements are carried out in the range of wavelengths 0.5-1.0 microns. UllNnO- CH 15 jf Yu 0.8 0 .7 O.S Of SM.n