SU595996A1 - Method for detecting and identifying choleric bibrions - Google Patents
Method for detecting and identifying choleric bibrions Download PDFInfo
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- SU595996A1 SU595996A1 SU762428613A SU2428613A SU595996A1 SU 595996 A1 SU595996 A1 SU 595996A1 SU 762428613 A SU762428613 A SU 762428613A SU 2428613 A SU2428613 A SU 2428613A SU 595996 A1 SU595996 A1 SU 595996A1
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- serum
- cholera
- vibrios
- agglutination
- nag
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
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Description
Изобретение относится к медицинской микробиологии, а именно к серологической идентификации холерных вибрионов.The invention relates to medical microbiology, namely to serological identification of cholera vibrios.
Известен способ обнаружения и идентификации холерных вибрионов путем проведения реакции агглютинации с О-холерной сывороткой [1].A known method for the detection and identification of cholera vibrios by conducting an agglutination reaction with O-cholera serum [1].
Однако холерные вибрионы в процессе изменчивости в организме человека или во внешней среде способны полностью утрачивать агглютинабельноегь этой сывороткой, и обнаружение и идентификация измененных форм холерных вибрионов и дифференциация их от других вибрионов становятся невозможными.However, cholera vibrios in the process of variability in the human body or in the environment can completely lose agglutinability with this serum, and the detection and identification of altered forms of cholera vibrios and their differentiation from other vibrios become impossible.
С целью определения измененных форм холерных вибрионов, утративших агглютинабельность О-диагностической сывороткой, реакцию агглютинации ведут с О-сывороткой к ΗΑΓ-MvraHTv vibrio cholerae 461/67—34.In order to determine the altered forms of cholera vibrios that have lost agglutination with O-diagnostic serum, the agglutination reaction is carried out with O-serum to ΗΑΓ-MvraHTv vibrio cholerae 461 / 67-34.
НАГ-мутант vibrio cholerae 461/67—34 получен экспериментальным путем из типичного штамма холерного вибриона классического биотипа и имеет следующие морфологические и физиологические характеристики.The NAG mutant vibrio cholerae 461 / 67–34 was obtained experimentally from a typical strain of cholera vibrio of a classical biotype and has the following morphological and physiological characteristics.
Клетки в виде изогнутых палочек величиной 1,5—2,0 X 0,2 — 0,4 мкм подвижны, с одним полярно расположенным жгути2 ком, спор и капсул не образует, по Грамму не окрашивается. На щелочном агаре Мартена pH 7,6—7,8 через 18 ч выращивания при 37СС образует полупрозрачные, глад5 кис, округлые, плосковыпуклые колонии диаметром 1,5—2,5 мм. В щелочной 1%-ной пептонной воде вызывает помутнение и образование пленки. Обладает индофенолоксидазной активностью, расщепляет до 10 кислоты без газа глюкозу, маннит, маннозу, сахарозу, крахмал; не ферментирует арабинозу, лактозу; декарбоксилирует лизин и орнитин, но не обладает дигидролазой аргинина; образует индол, лецитиназу; раз15 жижает желатину, не образует сероводород. Оптимум роста 37°С. НАГ-мутант vibrio cholerae 461/67—34 не агглютинируется О-, типоспецифическими, RO-холернымп сыворотками. При иммунизации кроликов 20 вызывает образование специфических антител.Cells in the form of curved rods 1.5–2.0 X 0.2–0.4 μm in size are motile, with one polar flagellum 2, do not form spores and capsules, and are not stained by Gram. At alkaline pH 7,6-7,8 Martin agar after 18 h of cultivation at 37 ° C forms a translucent glad5 Kis, spherical, plano-convex colonies with a diameter of 1.5-2.5 mm. In alkaline 1% peptone water it causes turbidity and film formation. It has indophenol oxidase activity, cleaves up to 10 acids without gas glucose, mannitol, mannose, sucrose, starch; does not ferment arabinose, lactose; decarboxylates lysine and ornithine, but does not possess arginine dihydrolase; forms indole, lecithinase; 15 liquefies gelatin, does not form hydrogen sulfide. The optimum growth is 37 ° C. The NAG mutant vibrio cholerae 461 / 67-34 is not agglutinated by O-, type-specific, RO-cholera serums. When immunizing rabbits, 20 causes the formation of specific antibodies.
Для получения агглютинирующей сыворотки кроликов весом 2,5—3,0 кг иммунизируют взвесью в физиологическом раство25 ре 18-часовой агаровой культуры вибрионов НАГ-мутанта vibrio cholerae 461/67—34, убитых кипячением в течение 2 ч. Животным вводят внутривенно 1, 2, 3 и 4 млрд, микробных клеток (по оптическому стан30 дарту ГИСК) с пятидневным интервалом.To obtain agglutinating serum, rabbits weighing 2.5–3.0 kg were immunized with a suspension in physiological solution of a 25-hour agar culture of vibrios of the NAG mutant vibrio cholerae 461 / 67-34 killed by boiling for 2 hours. Animals were injected intravenously 1, 2 , 3 and 4 billion, microbial cells (according to the optical standard30 GISK) with a five-day interval.
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SU762428613A SU595996A1 (en) | 1976-12-13 | 1976-12-13 | Method for detecting and identifying choleric bibrions |
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SU762428613A SU595996A1 (en) | 1976-12-13 | 1976-12-13 | Method for detecting and identifying choleric bibrions |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4705756A (en) * | 1983-01-26 | 1987-11-10 | University Of Medicine And Dentistry Of New Jersey | Method of determining the existence and/or the monitoring of a pathological condition in a mammal |
US4814247A (en) * | 1983-01-26 | 1989-03-21 | University Of Medicine And Dentistry Of New Jersey | Method for determining the existance and/or the monitoring of a pathological condition in a mammal |
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1976
- 1976-12-13 SU SU762428613A patent/SU595996A1/en active
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4705756A (en) * | 1983-01-26 | 1987-11-10 | University Of Medicine And Dentistry Of New Jersey | Method of determining the existence and/or the monitoring of a pathological condition in a mammal |
US4814247A (en) * | 1983-01-26 | 1989-03-21 | University Of Medicine And Dentistry Of New Jersey | Method for determining the existance and/or the monitoring of a pathological condition in a mammal |
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