SU638337A1 - P-9504 serotype 5 vibrio phosphoresceus strain as producer of standard agglutinating serum - Google Patents

Description

This invention relates to medical microbiology, namely to the serological identification of microorganisms.

The proposed strain is new, in patent 1 scientific and technical literature is not described.

Oil is a producer of typical agglutinated serum.

The strain Vibrio phosphorescens P-9504 serotin 5. is stored in the collection of the State Research Institute for Standardization and Control of Medical Biological Preparations. L.A. Tarasevich iodine number P-9504. Strain V. phosphorescens P-9504 isolated from humans and has the following morphological and physiological characteristics.

Culture lno-morphological features. The size of the cells of one-day agar culture (1.6-2.0) X (, 4) microns. Motile, with one polar flagella. Dispute and capsules does not form. Gram not stained.

Alkaline agar marten. Agar Hottinger.

M co-peptone agar (pH 7.6-7.8).

Translucent round colonies with a diameter of 2.0-2.5 mm, glowing in the dark.

Alkaline 1% peptone water. Diffuse clouding and gentle film, glow in the dark.

Physical and biochemical properties.

Propagated at 16-40 ° C. Opgimum growth at 36-37 ° C.

Glucose, mannose, sucrose, mannitol. maltose, starch, cleaves to acid without gas evolution.

Arabinose, lactose does not ferment.

Lysine and ornithine decarboxylates.

Arginine does not dehydrolyze.

Forms acetylaminophenol, indole.

Reduces nitrates to nitrites.

Thins gelatin.

Antigenic properties. When immunization of animals causes the formation of specific antibodies. Serum agglutinates cultures homologous and does not agglutinate cultures of heterologous serotypes.

Claims (1)

To obtain agglutinating O-serum of a given serotype of rabbits weighing 2.5-3.0 kg, they are immunized with a suspension in a physiological solution of an 18-hour agar culture of vibrios of strain P-9504, killed by boiling. for 2 hours Animals are immunized intravenously with 2-4-8 billion microbial bodies according to the optical standard of turbidity of gIsK with a five-day interval. 7 days after immunization, the rabbits are exsanguinated. Agglutinating serum is colorless, slightly opalescent. The titer of agglutinins 1: 1600-1: 3200. The proposed strain provides high-specific serum, non-agglutinating cholera, NAG - vibrios, as well as phosphorescent vibrios of other serological types and microorganisms of other species and genera of the family. Vibrionaceae. Claims of the invention: Vibrio phosphorescens strain P-9504 serotype 5 - producer of type agglutinating serum. Stored in the collection GISK them. Tarasevich under the number P-9504. Cultural and morphological features. The size of the cells one-day. agar culture (1.6-2.0) X (0.2-0.4) μm. Movable, with. one polar flagella. Dispute and capsules do not form. Gram not stained. Alkaline agar marten. Agar Hottinger. M co-peptone agar (pH 7.6-7.8). Translucent round colonies with a diameter of 2.0-2.5 mm, glowing in the dark. Alkaline% peptone water. Diffuse clouding and gentle film. Glow in the dark. Physiological and biochemical properties. Propagated at 16-40 ° C. Optimum growth at 36-37 ° C. Glucose, mannose, sucrose, mannitol, maltose, starch breaks down to acid without gas evolution. Arabinose, lactose does not ferment. Lysine and ornithine decarboxylates. Arginine does not dehydrolyze. Forms acetylaminophenol, indole. Reduces nitrates to nitrites. Thins gelatin. Antigenic properties. It has a specific O-antigen, distinct from both cholera vibrios, and vibrios from other serological O-subgroups and agglutinating with homologous serum.