SU576068A3 - Device for feeding plurality of individual quantities of liquid samples and reagents to multichamber conveyer disc - Google Patents

Description

perspective image of the device; in fig. 4 borrowing the reagent with the corresponding electrical compounds; in fig. 5 - interaction of the cam with the sample intake holder; and FIG. 6 - interaction of the cam with the reagent intake holder; in fig. 7 is a sample ring rotation mechanism.

The rotating or transfer disk 1 contains a number of cavities 2 locations, each section comprising a cavity 3 for the sample and a cavity 4 for the reagent. Using finger 5 in recess 6, disk 1 is rotated at a certain divided angle. Each section 2 is in line with a corresponding chamber 7 and contains external transfer cavities 8. j Cavities 8 are connected by channels 9 with corresponding chambers 7. A disk 1 is mounted on a rotating device 10, under which the light source is set AND, and under a transfer disk - photomultiplier 12. The disk 1 is mounted hinged 2o in the device 13 and contains a ring 14 for the sample. On the ring 14, circular holes are arranged with an equal gap between them, in which the cups 15 are placed for the sample, for example, for the bloodstream. 25

The holder of the sample intake diluent 16 and the holder of the intake of reagent 17 are connected through the cam 18 and the shaft 19 to the engine 20. The shaft 19 is mounted hinged on racks 21 to 22. The zaverny 23, 24 and 25 are initially set radially to the zakashchek 15 for the sample.

The cam 18 consists of two parts 18a and 18v, which are fixedly mounted on the shaft 19. On the holder of the sample intake diluent 16 and the holder of the reagent intake manifold 17, the plates 26 and 27 are jointly strengthened; 28 and 29. The cam 18 a has a groove 30 in which the roller 31 is placed, and the cam 18 in has a groove 32 and the roller 33. On the shaft 19, the cams 34 and 35 and the eccentric 36.40 are fixed.

Under the holder of the intake manifold 16 and 17 are located the tanks 37 and 38. On the shaft 19 there is an engine 20, which is connected through a connector to a pinch motor 39, which in turn is connected to a sample supply pump - dilute 45 bodies 40. The stepper motor has a cylinder 41 , drive rod 42, switch 43 with rod 44.

The disk 1 is mounted on the turntable 45, containing a roller 46 and a finger 47, which is included in the 50 notch 6.

The bristle ring 14 for the sample interacts with the finger 47 and is mounted on the turntable 45. In the lower part of the ring 14, a hole 48 is made instead of a notch. In the transmission disk 55, for the slashes 15 there are holes 49. The rotary roller 46 of the turntable 45 is mounted in the main roller 50, which is fixedly fixed relative to the engine 20. To the rotating roller 46 by means of the plate 51 is not- 60

movably fastened the ratchet pawl 52, Plate 51 have a finger 53.

Under the plate 51 there is a disk with holes 54 and 55. The plate 51 is connected to the eccentric 36 by a rod 56, a slipper between the stoppers 57. The switch 58 is connected to a scapular motor 39.

The transfer disk has a magnetic switch 59, a relay 60. A shaft 61 with a finger 62 is attached to the roller 46. A switch 63 and a relay 64 and 65 with buttons 66 and 67 are located under the transfer disk 1. The cam 35 is connected to microswitches 68-70. The switch 43 of the scap motor 39 connects the relay 71. On the shaft 19 there is a switch 72.

The device works as follows.

A sample is placed into cavity 3, and a reagent is placed into cavity 4. The loaded transfer disk 1 is rotated at a certain angle horizontally with a finger 5 and installed in the rotating device 10 so that each row of cavities, i.e. each section 2 of the cavity arrangement, is in line with the corresponding chamber 7. The sample and reagents are displaced and transmitted from cavity 8 through channels 9 into corresponding chambers 7. Chambers 7 filled with a mixture, rotate, pass between light source 11 and photomultiplier 12. Signals emitted by photomultiplier 12 indicate any change in passage and light due to the reaction between the reagent and the sample. When the button 66 is pressed, the device is energized, and when the button 67 is pressed, the analysis process starts, in which the reagent and diluent are fed successively to the desired section 2 of the cavities on the disk 1. A sample comes out of the slashes 15, the reagent goes from the tank 37, and from tank 38 - thinner.

When the button 67 is pressed, the relay 64 is turned on. In the initial indexed position of the device, the relay 64 is closed with a finger 62, which is located on the rod 61.

Relay 64 is connected so that it remains on after button 67 is turned off. Thus, the relay is connected automatically. When the relay 64 is turned on, the power from the motor 20 is fed through a connector to a conventional stepper motor 39, which is connected to a diluent sample supply pump 40.

The sample feed diluent pump 40 includes a micrometer that is connected to the stepper motor shaft. When the button 67 is turned on, the engine 39 moves the cylinder 41 upward, forcing the intake 23 to take a diluent from the tank 38, held by the holder 16 and the tube. The holder 16 with the sample intake 23 remains until the drive rod 42 comes into contact with the rod 44 of the switch 43 and causes the single-pole switch to close in two directions. The circuit breaker 43 turns on

the relay 71 through the lines, stops the stepping motor 39 and automatically triggers the relay 65 which provides power from the connector through the lines to the motor 20, which starts rotation at a uniform speed. The switch 43 is positioned relative to the drive rod 42 so that contact is made. When cylinder 41 takes a sufficient amount of diluent from reservoir 38, all samples to be analyzed are diluted. In this case, the switch 43 will not be commissioned by the rod 42 until the intake takes at least 1.65 ml of diluent. When the rod 56 is actuated, which is accomplished with a sliding connection to the eccentric shaft 36 at the end of the loading cycle, the plate 51 is raised to release the pin 53 from the engagement with the hole 54 and move to the right for the engagement with the hole 55. As a result, the eccentric 36, continuing to work on the rod 56, rotates the table 45, the ring for sample 14 and the transfer disk 1 to the left, so that the other plate for the sample 15 and the reagent cavity 4 and its cavity 3 and for the sample 3 should be placed radially in line with the bottom Rniki held by the holder 16 and 17.

When the engine 20 is turned on and running in an unregulated manner, the cam 18a turns. As a result, the diluent sample intake holder 16 is moved (the cam position is when the intake is in the position indicated by the arrow in Fig. 5). In this position, when the intake 23 is immersed in the sample cup 15, the switch 58 is closed using a foot actuator fixedly mounted on the plate 26. The circuit of the switch 58 switches on the stepper motor 39 which rotates in the same direction as originally. The rotation of the engine 39 ensures the operation of the pump 40 and thereby the sampling.

A switch 58, which was open when the diluent sample holder 16 moved, was turned on with a foot actuator. As a result, the motor 39 is turned on again and rotates in the direction opposite to its initial rotation. The cylinder 41 moves downward and outputs a predetermined portion of the sample and diluent contained therein, i.e. the total amount. The amount of the sample and the diluent, which is fed into the cavity 3 for serum, is controlled by a second finger switch, which stops the stepper distributor 39, after its rotation for a predetermined number of steps. The holder 16 and the intake 23 are moved back (i.e., to the position corresponding to the position of the intake indicated by the arrow in FIG. 5). In this position, sample intake 23 is washed from above with diluent in tank 38. The switch closes

if holder on his return to the original position.

In this case, the reference circuit switches to the initial position, e

Immediately before returning the holder 16

to its original position, the cam 36 begins to move the rod 56, which causes the ratchet pawl 52 to turn the table 45 to feed the next cup 15 for the sample

opposite the holder 16 of the sample inlet - diluent. When the holder of the reagent intake manifold 17 is in its original position (the cam 34 operating in the supply of the reagent, water, indicated in Fig. 6 by the arrow, will interact

with spring, roller, roller and plunger, moves the reagent syringe plunger to the right, thereby allowing the reagent to be sucked into the intake 24. The amount of reagent sucked into the intake 24 is controlled either by switch 70 or

switch 68. The choice of switch 68 bus 70 is determined with a switch; the volume selection switch is controlled by the volume selector switch. switch that is set manually until the button 67 is turned on, depending on whether

whether or not to issue a command — take a relatively larger or relatively smaller amount of reagent for the analysis being performed. When it is necessary to collect a large amount of reagent, the selector switch is set to the closing position of the switch 68 by means of the relay 60. Thus, when the holder 17 of the reagent intake is located at the position indicated in FIG. 6 arrow, cam 35 operating on microswitch, snaps

the switch 68, when the end of the cam 35 comes into contact with the switch 68. The closure of the switch 68 provides an electrical signal for opening the stroke to the die plate of the grate 24. The supply of the entire reagent containing the intake 24 to the cavity 4 and the preparation for the subsequent operating cycle is provided by suction a small volume into the cylinder (Fig. 4) for the reagent by moving the plunger to the right (Fig. 4) during the rotation of the cam 34,

Reagent supply agent - water :. During this time, the reagent intake manifold 17 returns to its original position, corresponding to the position of the intake manifold indicated by the arrow in FIG. 6, due to the interaction of the cam 18 a.

The air that is in the cylinder (Fig. 4) for the reagent, works to displace the entire reagent from the inlet 24. After feeding the reagent into the cavity 4, which means the end of one

turning the rotary shaft 19, the reagent sampling holders 17 and the sample intake holder 16 are moved back to their original position to load all the cups with the sample and reagent. At the end of the loading cups 15 on the ring rod 61, connected to the roller 46, making a full

the rotation with the protrusion 62 closes the switch 63. As a result, the relays 64 and 65 are turned off. The motor 20 remains under power, which is fed through the lines through switches 63 and 72. When the switch 72 closes, which occurs with the aid of the dilution holder 16, when oi returns to its original position after the switch 63 closes, the power of the engine 20 is interrupted, and the duty cycle is terminated.

Claims (2)

1. US Patent No. 3594129, cl. G 01 N 1/10, 1971 2. Journal of Analytical Biochemistry №28, 1969, p. 545-562. / V / " Theb.6