SU562772A1 - Method of testing neuro and psychotropic drugs - Google Patents

Description

(54) METHOD FOR TESTING NEURO AND PSYCHOTROPIC MEANS

I

The invention relates to the field of medicine and relates to the pharmacological testing of neuro and psychotropic drugs that can be used for liver Parkinsonism, depression and some other nervous and mental diseases.

A known method for the investigation of neuro-and psychotropic drugs containing L-3 -4-dio-Xyphenylapanine (L-DOPA), which consists in administering the preparation to spinal rats and studying the contraction reflex of the hind limb 1.

However, this method does not provide high specificity and sensitivity, in addition, it requires prior surgery on the animal

The aim of the invention is the elimination of operative accommodation on the animal and ensuring the possibility of its reuse.

This is achieved by using a tropical plant as an experimental animal, whose motor activity is determined by synergistic hind limb shocks, and the dopamine biological activity of the test substance is judged by the number of strokes per unit of time. The essence of the invention is that the administration of L - DOPA in the blood in doses of 10-100 mg / kg causes a behavioral reaction in rabbits, expressed by synergistic hind-paws. This form of rabbit behavior is based on a quantitative indicator, a test response of the state of emotional stress and specialized motor activity of the animal. This test reaction is highly specific for L-DOPA.

After injection into the blood C - DOPA test h

the reaction occurs after 1–3 minutes and

Duration of ten minutes with an average frequency of 1.8 hits per 3 minutes. In the control, i.e., after administration of a physiological solution or without administration of the preparation, the indicated test is

the reaction is characterized by an average frequency of 0.01-0.07 shock for 3 minutes.

Introduction to the blood of central nervous system stimulators, catecholamines, reserpine and other drugs does not cause

the occurrence of this test reaction. This test reaction is highly sensitive to L - DOPA, the threshold dose L of DOPA is 5-10 mg / kg, intravenously. The proposed method consists of the following four operations. First, the number of rabbits' hind legs was measured in the control. The specific activity of L-DOPA is then determined by introducing L-DOPA and measuring the indicated test reaction. After that, the same reaction is measured after administration of the test drug. Then said tesg is measured after the introduction of L-DOPA against the background of the effect of the medicinal product, and L-DOPA is administered in the same way.

and in the same dose as in the second operation, and the activity of the studied drug is judged by quantitative indicators.

All these operations are performed on one or two groups of animals.

Example 1. Determination of the specific activity of the drug L-DOPA.

Select a rabbit weightZ-4kg,

who had not been in the experiments before.

Prepare a 2% solution of L-DOPA. After that, each animal is placed in a separate box to measure the number of hittings with its hind legs. The number of strokes on the hind legs in the control is recorded on the self-writing device. (not less than 18 min.) If during this time at least one beat is recorded, the recording is continued until there is not a single blow during 9 minutes. Then, a 2% L-DOPA solution is injected into the marginal vein of the rabbit, and for each animal the dose of L-DOPA is 50 mg / kg. After that, the number of beats by hind paws is recorded until the end of | 5action on the introduction of L-DOPA, the recording is continued for at least 18 minutes. At the end of the study and process the data obtained. On the basis of the test results, a conclusion is made about the high specific activity of the drug L-DOPA.

 Example 2: Testing the psychotropic activity of caffeine, sodium benzoate.

Randomly selects a new group of rabbits weighing 3-4. kg A 2% solution of L - DOPA is prepared. Each animal is placed in a separate box and the number of beats is measured in the rear.

with the paws first in the control, then the indicated test is measured - the reaction after the intravenous administration of a 2% solution of L-DOPA (50 mg / kg). All measurements are carried out in the same way as described in

The results of the test of CoffetK-rtati-H beMzooit s were compared with test-reaction measurement data in the control and after administration of L-DOPA obtained on the same group of animals, as well as data obtained on other groups of animals. These comparisons show a high eraticistic reliability and accuracy of the test results. On the basis of the test, it is concluded that SoPuifo Co {fei-nai} i benzoat s (2%, 2.5 mp) upon intravenous administration strongly potentiates the specific effect of L DOPA.

Comparison results provide an opportunity to conduct tests on two different groups of animals, as well as to carry out an express test.

In the latter case, the test response is measured only after the administration of the test drug is administered, and then the same test response after the administration of L-DOPA, which are previously determined on a large group of animals, is measured.

The preparated method will find application in the pharmaceutical business, for example, in determining the authenticity of medicines containing L-DOPA and assessing the quality of medicines, as well as in pharmacological testing of neuro - and psychotropic drugs.

Claims (1)

Invention Formula The way of testing is neuro-and psychotropic: {. agents containing L-3-4-dioxiphenylalanine (L-DOPA), by administering them to an animal, followed by measuring the motor activity of the hind limb, characterized in that, in order to increase specificity and sensitivity: the method, the test substance is administered to the rabbit and motor - Example 1. Then after 48 hours, a second control is performed on the same group of animals. After that, each rabbit is intravenously injured with 2% SoPutioCoffelHJ-natrii beiitzoatis, 2.5 ml each, i.e. only 50 mg of the drug, then the number of hind legs was measured before the reaction was stopped, the measurements were carried out for at least 18 minutes. 30 minutes after the administration of CoffeH t -natl- (bettzoai s, 2% solution of LI-DOPA was intravenously injected into rabbits at the previous dosage, i.e. 50 mg / kg, and the number of hind legs was measured again until all experimental animals Za5 2772g The activity is determined by the synergistic Uda-Sources of information that are common but back-to-back, and by the number of Uda-manna during examination: ditch in units) .- time judged by dopamine l.N.H.Awden ei and Euf opeoin ZogpaS biological activity of a substance. of phat mactiEo y, v2 i9o7,