SU539072A1 - Yeast-like fungus strain -55-13 producing glucoamylase - Google Patents

Description

one

The invention relates to the microbiological industry, more specifically to the production of a new yeast strain used to form the enzyme glucoamylase, for example, in the production of minerals.

Various strains of the yeast-like fungus Endomycopsis iibuliger are used to form the enzyme glucoamylase 1.

The known strains of the yeast-like fungus have low glucoamylase activity, which prevents the preparation of the process and an increase in the yield of the product.

The purpose of the invention is to produce a new strain with persistent bno.himicheskimi properties, they have a high capacity for glucoamylase, and therefore an increase in the yield of products and intensifies the production process.

Strain d) of the endomycopsi iibuliger 55-13 burnout fungus — the glucoamylase producer was isolated as a result of a threefold combination of the mutagen of ethyleneimine co1n; 1, 2000 for 30 minutes and ultraviolet rays. Rays at a dose of 4, OXiO erg / mm — at the first step of 2.0x10 erg / mm — at the second step of the third step of selection.

2

Morphological feature and.

On a malt wort at 30 ° C, the 48-hour culture forms yeast cells in osovoy oval shape and, less commonly, rounded, measuring 1.75-5.5 X2.0-3.5, with an average of 4.95 X 2.7 microns. In large quantities, seed branched hyphae with a diameter of 1.5-2.5 are formed, with an average of 2.05 µm.

On synthetic medium, the shape of the cells is the same as on malt wort. The cell size is 2.5-6.25 X1.5-3.75, an average of 3.75X X2.67 microns. Hyphae with a diameter of 2.5-3.25, an average of 2.75 microns.

During deep culture for 76 hours, mycelium hyphae completely disappear under conditions of intensive aeration, and the proliferating cells remain smaller.

Kultaws Prize and

Growth in railway media.

On a malt wort after 48 hours at 30 ° C a ring is formed. After 3-5 days, a thick plop is formed, covering the entire surface. At the bottom of the tube sediment micelle in the form of fluff. Without agitation, the medium is transparent.

On the medium, the ring is expressed in pche 11 medium is turbid without shaking.

Growth in solid media.

Nosev p trph on wort agar.

Growth is powerful, white, dense, dull, wilted. The surface is mealy. The edge is lightly wavy, fleecy.

On a synthetic medium - the growth is the same as on wort agar, the edge is finely wavy, fuzzy with wrinkles.

lioceB prick on malt agar.

Growth on the surface of the injection medium, white, dull. The prick is weak, branched with a ruptured environment with gas formation.

Giant columns after 7 days with 20 malt agar — round, white, 12–13 mm in diameter, dim, slightly watered, smooth edge, shaggy, and mealy surface, b 3-4 mm thick crater colony, surrounded by a small roller with a diameter of 0 5 mm. In the crater and the surface is finely lumpy. There are two concentric circles.

On the synthetic medium, the colonies are round, smaller, And -12 mm in diameter, dim, white, slightly convex. In the center of the colony there is a platform with a diameter of 5 mm and a large deepened. Powdery surface with a pronounced concentric circle. The edge is even, fleecy with a growth border of 0.5 mm.

B and about X and m and c of with to and e and r and a sign and.

Hydrolyzes starch, does not restore nitrates, milk coagulates and hypothesizes, dilates gelatin weakly.

Relation to nitrogen. Use iepton, urea, weaker asparagine, calcium nitrate. Ammonium sulphate is not absorbed.

Relation to carbon sources. It uses sucrose, GLUcoza, maltose with the release of gas, it assimilates less arabinose, lactose and galactose. Does not use organic acids and mannitol.

The optimal temperature for the biosynthesis of glucoamylase and pH 5.5.

The strain of the yeast-like fungus Endomycopsis fibuliger 55-13 was tested for enzymatic activity during storage and passaging on medium, composition,%; potato starch (soluble) 1.0; corn extract 1.0; glucose 0.5, potassium monophosphate 0.5 and agar-agar 2.0.

For submerged cultivation, the same medium is used, instead of potato starch, corn native starch is taken.

Growth of the yeast-like fungi Endomycopsis libnliger 55-13 under the conditions of submerged cultivation on a rocking rod of 180 rpm and AIA is intense, mushy.

The maximum formation of tlyukomylazy in the culture fluid is observed on the second or third day of cultivation.

When nassing and snoring, a high level of enzymatic activity of the strain Еdomdomycopsis fibuliger 55-13 does not disguise / repent.

Glucose activity was determined by the glucose oxidation method.

The results show that the activity of the mutant Endoraycopsis fibuiiger 55-13 on this medium is 14.0–16.0 µm / ml-min, i.e., 10–14 times greater than that of the strain of consumption, with the highest activity glucoamplase is observed in acidic

Sope irp pH 5.5.

Thus, the actoinum of the glucoamylase strain of the yeast-like fungus Endomycopsis fibuhger 55-13 - iroduceitis glucoamylase exceeds glucoamylase activity

a strain of lime and is a new culture with a stable biochemical properties, consisting in enhanced ability to glucoamylase biosynthesis.

F o rmu l invention

The strain of the yeast-like fungus Endomycopsis fibuliger 55-13 - but the producer of glucoamylase.

Morphological features. On the malt wort at 30 ° C, the 48-hour culture forms drone cells mostly oval and less commonly rounded, 1.75-5.5 X2.0-3.5, with an average of 4.95 X 2.7 microns. Slotted branched hyphae with a diameter of 1.5-2.5 are formed in large numbers, on average 2.05 µm. On synthetic medium, the shape of the cells is the same as on malt wort. The cell size is 2.5-6.25 XI, 5-3.75, with an average of 3.75X2.67 microns. Hyphae with a diameter of 2.5-3.25, an average of 2.75 microns.

Under in-depth cultivation for 76 hours under conditions of intensive aeration, the ntsely nfs completely disappear, and the yeast cells remain smaller.

K u lt t r a l n s signs. Growth pas liquid media.

On a malt wort after 48 hours at 30 ° C a ring is formed. After 3-5 days, a thick film is formed covering the entire surface. At the bottom of the pot there is a precipitate in the form of fluff. Without shakewap w Wednesday transparent. On the medium, the ring is expresicid and the medium is turbid without agitation. Growth pa solid media.

Sowing shprpom pa wort agar. Growth of moshch, white, nlotny, dim, convex. The surface is mealy. The edge is finely wavy, fleecy.

On a sitétic medium, the growth is the same as on a slug-agar, the edge is finely wavy, shaggy with a bulge.

Inoculation of malt agar. Growth on the surface of the injection medium, white, dull. The prick is weak, branched with a rupture of the medium with gas. After 7 days at 20-22 ° C, the malt agar was round, white, with a diameter of 12-13 mm, dim, slightly convex, the edge of a rosy, fuzzy, surface of mealy. In the center of the colony there is a crater with a diameter of 3-4 mm, surrounded by a small roller with a diameter of 0.5 mm. In the crater, the surface is finely lumpy. There are two concentric circles.

On the synthetic medium, the colonies are round, smaller, dimetre. II -12 mm, dim, white, slightly convex. In the center of the colony area with a diameter of 5 mm with a small recess. Powdery surface with a pronounced concentric circle. The edge is even, fleecy with a growth border of 0.5 mm.

B and X and. M and the c and e sign and.

Hydrolyzes starch, does not restore nitrates, milk coagulates and pentonizes, dilutes gelatin weakly.

Relation to nitrogen. Uses peptone, urea, weaker asparagine, Caliiifi nitrate. Ammonium sulphate is not absorbed.

Relative to carbon sources.

It uses sucrose, glucose, maltose with the release of gas, it assimilates less arabinose, lactose and galactose. Does not use organic acids and mannitol.

The optimal temperature for glucoamylase biosntaz is 30 ° C and pH 5.5.

The source of information taken into account is:

1. Copyright certificate L. 340690, M. Kl. From 12D 13/10. 1972.