SU538022A1 - The strain of the fungus m-45 pectolytic enzyme producer - Google Patents

Description

one

The invention relates to microbiological production / development, in particular, to a new strain-producer of pectic enzymes used in the wine-making, canning and other industries.

Various strains of Asperg-iCEus rib 1 are known, for example AspergifEus loetidus (initial), producing enzymes of the pectolytic complex in surface and deep cultivation methods l.

The enzymatic activity of a known strain is insufficient to ensure the biosynthesis of pectolytic enzymes.

The purpose of the invention is to obtain the AspergriEEus foeiidus mold robbery strain, which has an increased ability to biosynthesis of pectolytic enzymes in the deep cultivation method.

The strain of the fungus Aspercr-f 6t s f oc-tidusM-45 was isolated as a result of stepwise selection using mutagenic factors of chemical nature, it is stored in the collection of microorganism cultures of the All-Union Scientific Research Institute of Genetics and Selection of Industrial Microorganisms; registration number T -81,

Morphological signs.

In carrot agar, mycelium is very strong; en; drug with thin hyphae, thicker with short cells are found, the cell walls of the mycelium are straight or slightly isanlisty. A thickening of the cells is observed near the septa. The length of mycelial cells varies from 7-27.5 to -15-55 microns. Conidiophores are straight or slightly curved, brown. Conidia are round brown with thick spinous shells 3.75-5.50 microns in diameter,

Cultural features.

On carrot agar, colonies are round white with deep radial winding folding. Conidiene formation is very weak. The diameter of giant colonies on the 6th day of growth reaches 70-75 mm.

Claims (1)

On an agar medium of čapek C, 6% is stored flat and 1O% extract from malt sprouts of a white colony are round and only in the central part slightly elevated above the substrate with rough folding. The rest of the surface of the colonies with sinuous radial folding. The conidium formation is insignificant, barely noticeable only in the center. The diameter of the colonies on the b day of growth is 53-55 mm, the edges of their large dentate. On slices of potatoes, growth is good, folded, conidia are weak. Physiological signs. M co-peptone gelatin liquefies. Milk does not coagulate and does not apply, nitrates restores. Starch hydrolysis em. Attitudes towards carbohydrates: glucose, galactose, xylose, maltose, and also mannitol hydrolyzes with the formation of alkali. Sugar, lactose hydrolyzes, intensely acidic medium. Gas formation is not observed in any of the listed media. The preparation of the active strain AspergriCfus loc-tidus M-45 is carried out by preparing material for breeding, isolating the monoconidial cultures of the original AspefgriESos foe-tidus strain, studying their natural variability for the formation of enzymes and selecting the best strains that then serve as starting cultures for subsequent research. Then, exposure is carried out with chemical mutagens nitroso-E: nitrosomethylurea (NMU) and nitrozome tilbiuretom (NLM) at the selected conidia monokonidialnoy culture of the original strain sieving AspergrieCus foctidus, isolated a large number of options and wasp schestvl dissolved sieving desired mutant. A new strain of A & perg-i6fiy§ foeiid (JSM-45 was isolated after treatment of the conidia of the original strain with 0.1% nitrosomethylurea for 2 hours. The active strain AspepfftfCus foe-ti3usM-4 was subjected to enzymatic activity testing during storage at resets agar nutrient medium composition,%: glucose 2.0, ammonium nitrate 0.3, potassium phosphate monobasic 0.1, magnesium sulfate 0.1, iron sulfate traces; carrots 50.0; agar-agar 20, Cultivation of the strain in deep conditions They are carried out on a liquid medium of composition,%: extract from beet pulp 89.15; Toxic from malt sprouts 10.0; ammonium sulphate 0.7, potassium phosphate monobasic O, 1O, magnesium sulphate 0, O5; pH of 4.1-4.3. Growth of the strain of the fungus Asperg-iEEus fo-aiidus M- 45 on this medium under conditions of deep cultivation on a rocking chair at 180 rpm and 30 ° C intense, has the appearance of a thick fine-grained, pasty mass. The maximum formation of pectolytic enzymes in the culture fluid is observed after 66 h of growth. Indicators of pectolytic activity {pectinosterase and polygalactic) are determined by the interferometric method, they are 1.4-1.6 units / ml of culture liquid, and the indicators of the activity of the original strain do not exceed 0.70, 75 units / ml. A two-year study found that during storage and passaging on carrot agar, a high level of enzymatic activity of the strain AspepgrifCus foe-tidus M-45 does not decrease. Thus, the proposed strain, compared with the original culture, has the advantages of enhanced biosynthesis of the pectolytic complex of enzymes, and is a new valuable culture with persistent, hereditary fixed biochemical properties. Claims of the Infection Aspepcrius fos & clidusM-45 strain producing pectolytic enzymes are stored in the collection of microorganism cultures of the All-Union Scientific Research Institute of Genetics and Selection of Industrial Microorganisms. Registration number T-81. Morphological features. On carrot agar, mycelium is extensively branched; along with thin hyphae, there are thicker cells with short cells. cell walls of mycelia direct or slightly sinuous. A thickening of the cells is observed near the septa. The length of the mycelial cells varies from 7-27.5 to 15-55 microns. Conidiophores are straight or slightly curved brown. Conidia are round brown with thick spinous shells with a diameter of 6.75-5.50 microns. Cultural features. On carrot agar, colonies are round white with deep radial winding folding. Conidiene formation is very weak. The diameter of giant colonies on the 6th day of growth reaches 70-75 mm. On an agar medium of čapek C, the 6% collapse is small and the 10% extract from malt sprouts is white, round, flat and only in the central part slightly elevated above the substrate with hilly skpadch-ate. The rest of the surface is a colony with sinuous radial folding. Conidiene formation is insignificant, barely noticeable only in the center. The diameter of the colonies on day 6 of growth is 53-55 mm, the edges of their large dentate. On slices of potatoes, growth is good, folded, conidia are weak. Physiological signs. M co-peptone gelatin liquefies. Milk does not roll and does not peptonize, restores nitrates, hydrolyzes starch. Attitudes towards carbohydrates: glucose, galactose, xylose, maltose, and also mannitol hydrolyzes with the formation of alkali. Sucrose, lactose hydrolyzes, intensively acidified medium; No gas formation is observed in any of the listed media. Sources of information taken into account in the examination: 1. Los Kova LS, Rudenko TP, Strunnikova TR, Sin kin AA Become a study of the conditions for the biosynthesis of pectological enzymes by the mold fungus AspewECus foeiidus in the works of the enzyme department. Issue 1. Enzymes, production and use in the food industry, ed. 1972 - the prototype.