SU535914A3 - The method of detection of urobilinogen - Google Patents

Description

high content of urobilinogen - carmine-red.

The same color reactions are produced by indicator paper containing 0.3 g of the following diazonium vessels:

fluoroborate 2-met10-oxibenzoldiazonium; 2-hydroxybenzene diazonium sulfate; 4-hydroxybenzene diazonium sulfate; 2,4-dioxybenzene diazonium fluoroborate. Example 2. Filter paper (Schleicher and Schuill 23 SL) is produced with a solution of the following composition, g: Fluoroborate 4-methoxybenzene diammonium 0, 3

Oxalic acid10.0

Then it is dried at 40 ° C. Example 3. Filter paper (Schleicher and Schull 23 SL) is ironed with a solution of the following composition:

4-Ethoxyaniline, G0.25

Isoamilonitrile, ml1,0

Shavel's acid, g10,0

Sodium salt dodecylbenzenesulfonic acid, g0.4

Methanol, mlto 100

Then it is dried nri. This indicator paper reacts with urine containing: urobilinogen, with the formation of carmine-red color.

Violet is tinted, to indicator paper gets, if 4-ethoxyaniline is replaced with equal amounts of 2-ethoxy-, 4-butoxy-, 4-phenoxy-, 4-methoxy-2-methyl-, 2-methoxy-5-methyl, 2-methylmercapto- 4-methylmercaito-4-chloromethoxy-5-methyl-, 5-chloro-2-hydroxy- or 4-benzloxyaniline.

Example 4. Filter paper (Schleicher and Schull 23 SL) is impregnated with a solution of the following composition: I. Fluoroborate 4-acetamido benzene diazonium, h0.3

Sodium metaphosphate, g5.0

Water, mlDo 100

P. SheVeleva acid, g10,0

Lauryl sulfate "atri, g0,5

Methanol, mlto 100

Then it is dried.

This indicator paper reacts with urine containing urobilinogen, with the formation of carmine-red color.

A similarly impregnated Z-methoxy-4-acetamido-iso-i-zondiazonium fluoroborate paper reacts with a red-violet color.

Example 5. Filter paper (Schleicher and Schull 23 SL) is impregnated with a solution of the following composition:

Z-chloro-4-methoxybenzene diazonium toluene sulfate, g 0.5

Sulfosalicylic acid, g5,0 Dodecylbenzenesulfonic acid (sodium salt), g 0.8 Methanol, ml Up to 100

This indicator paper, when immersed in urine containing urobilinogen, gives a carmine-red color. When immersed in the urine, containing bilirubin, after 10-20 seconds, a blue-green o-paint is obtained. Urine containing urobilpnogen and bnlirubin, according to their content, gives a half tone between these two stains.

PRI me R 6. Polyester fiber is impregnated with a solution of the following composition: Benzenediamopium salt, g0.1

Sulfosalicylic acid, g10.0 Patriev dodecylbenzenesulfonic acid salt, g0.4

Methanol / water 1: 1, ml Up to 100

The following diazonium salts are used: 2-methoxy-5-chlorbeisol Diazonium tetrazincate (solid red RC salt); 4-benzamido-2-methyl-5-methoxy-isyldiazonium tetrachlorozinc (strong violet salt R);

4-benzamido-2,5-dimethoxybenzene diazonium tetrachlorozincate (strong blue salt of RR); 4-benzamido - 2,6 - diethoxybenzene diazonium tetrachlorozinc (durable blue salt);

4 - methoxyphenylaminobenzene diazonium hlurmd (variamine, blue salt B).

When the reaction of sulphate of acid phenylaminobenzene diazonite with urine containing urobilinogen, the following colorations are obtained: red-blue; red and purple; blue-violet; blue-violet; blue-green; blue-green.

Example 7. Filter paper (Schleicher and Schull 23 SL) is impregnated with aqueous solutions, in which, in addition to 5 g of sodium sulfate, there is up to 0.5 g of the following diazonium salt and 100 ml: 2,4-dimethoxybenzene diazonium fluoroborate; 3,4-dimethoxybenzene diazonium fluoroborate; fluoroborate 2,5-dimethoxybeisoldione;

Fluoroborate 3,4,5-trimethoxybeisolindazoni; 4-hydroxy-3,5-dichlorobenzene diazonium fluoroborate; 5-chloro-2,4-dimethoxybenzene diazonium fluoroborate.

All of these indicator papers react with urine containing urobilinogen, forming from red to purple-krayoy color.

If the indicator paper impregnated with a 1% solution of the following wetting agents, the solid obtained from red-purple to blue-violet color: dodecylbenzenesulfonic acid (sodium salt) in methylene chloride, sodium lauralsulfat chloroform - methanol dodekaisulfokislota (sodium salt) in chloroform - methanol, dioctyl sodium sulfosuccinate in methylene chloride.

Example 8. The filter paper (Schleicher and Schull 23 SL) is permeated with the following content:

5-aminosalicylic acid, g

Metaphosphoric acid, g

Sodium nitrite, g Sodium dodecylbenzenesulfonic acid, g Methanol, ml

Water, mlDo

Indicator-paper reacts with urobilinogen containing, with the formation of a brick-red color. Paper, impregnated with 5-amino-Z-sulfosalicylic acid, reacts to form a cyrillic-red color.

Claims (2)

1. A method for detecting urobilinogen, nairimer, in the urine, based on a color reaction when the sample under investigation interacts with a carrier treated with a mixture of indicator, acid, surfactant and stabilizer, characterized in that, in order to increase the sensitivity and enable the determination of urobilinotena in the presence of bilirubin, as an indicator, benzoldiazonium salts of the general formula are used 15.0 0.1 where RI is oxy-, alkylmercapto-, lower alk100 urine. hydroxy groups; iR2-OXY-, alkoxy, phenoxy, aralkoxy, alkylmercapto, phenylmercapto, phenylalkylamino groups, substituted phenylamino or acylamino groups; wherein one of the residues RI and RS can also be a hydrogen, halogen or methyl group; Rs and R4 are hydrogen, halogen atom, hydroxy, alkoxy, phenoxy, alkyl mercapto, phenyl mercapto, phenylalkylamino, substituted phenylamino, acylamino, alkyl, sulfo and carbocoyl groups; ll X-chloride, sulfate, tetrafluoroborate, tetrachloro cyanate, hexachlorantimonate, aryl sulfonate or carboxylic acid residue. 2. A method according to claim 1, characterized in that metaphosphoric acid is used as the acid.