SU521879A1 - The method of obtaining protein-vitamin food fortifier - Google Patents

Description

(54) METHOD FOR GETTING EELKOVITA-VITAMIN FOOD PROTECTOR

Invent vK; -,; i9 from Osigs,; m Efbibiopogicheskoy .tip Mb / i jiertHfAVTii to. Shchse oyt applied to npouBBiJa-Ji-Bi without JiKi B & Vitamin-nzhh enriched. through their fermentative hydrolysis, which are light, well washed, yeast is heated for S at S for 2O – 3 min for denauratsy badges, .. After this, the fermenter in the repvie H4HOM fermentor in an inert medium, The process of idol is in sterile conditions with .1Leg: BathE.}: LiiapvieHTHor iipenapais Oggzi:: within 24 hours. It makes us know about boron amy ocaslo: r, among the cohorazhatas, which are not replaceable. -etyj; that the enzyme is a “hot” nucleus to:;; it is carried out by the enzyme npertapa rciif / shaosStilin TZH., which has a corresponding activity, units, g / g: Ba. It also allows the use of the enzymes of the yeast KopMOEctie hydrolysis for protein hydrolysis. nye yeast immediately and: separation ssle (75-9O% humidity) was heated to 48 ± 2 C and was added fermentnyi preggarat Ar zosubtilin GZH. The process is carried out in a heap of 2O-30 hours with weak stirring,. Then, the hydrolyzed yeast is centrifuged, the bright fugate, which contains the amino acid-lower peptides and vitamins; drained and dried under vacuum. Example. About 15 g of fodder yeast of the Leningrad hydrolysis plant, grown on g-., Lysates of wood, brown, 1 with 8C% moisture, are placed in a c-к flask per 25O ml. Cob; put into a water bath with a temperature of 4 ° C, in which Oj71 g is introduced enzymatically: 5 preparations of Lysosubtilin GZH. To evenly distribute the drug.

in the flask, the mass is stirred-t (the stirrer makes about 1OO rpm). After 29 hours, the hydrolyzed yeast is centrifuged at 8OO rpm for 2 O min. The centrifuge, which is about 5O% o6i from the contents of the flask, is drained and dried under vacuum. The centrate contains 37 O mm / ml of amino nitrogen, which is 50% represented by the following L-amino acids, MAP.% To the sum of amino acids:

Lysine14,3

Histidin1, O7

Aspartic Acid 3.88 Threonine7.33

Serin7,12

Glutamic acid 5.67 Proline5.38

Glycine4,85

Alanya21,6

ValinO 88

Cysteine 6,66

Methionine1,91

Isoleucine 5,01

Leucine11.4

Tyrosine1.14

Phenylalanine3,83

Total1OO%

The remaining amine nitrogen is a fraction of the lower peptides. With the need for a product with a different ratio between peptides and free amino acids, the following process parameters vary: the concentration of the preparation is lysosubtilin GZH, the process time, the pH value in the hydrolyzable material.

The resulting product has a rich aminoacid activity, u / g:

acid composition and suitable for long-term Bacteriolytic 400OOO-85OOOO

stored. Straw yellow color and at t-amylazene 200-300

The new food odor allows Proteinase 15OO-25OO to be used.

This plant is a protein-vitamin extractor of various types of food, especially lean protein, for example, mealy products. If necessary, in the final product a mixture of L-amino acids can be separated from other substances, for example, by precipitation on ion exchange resins.

The absence of multistage and the need for sealing, as well as in inert gas, considerably simplify and cheapen the process. Lnzosubtilin GZH, and fodder hydrolysis yeast are one of the cheapest and most affordable and currently produced on an industrial scale, which makes it possible to put the production of protein-vitamin fortifiers on a profitable industrial basis. Unhydrolyzed cell residues containing foam nutrients can also be used in the feed industry or in agriculture.

Claims (1)

Invention Formula The method of obtaining protein-vitamin fortifier write by enzymatic 30 hydrolysis of yeast biomass, characterized in that, in order to obtain a product containing a complex of essential amino acids, as well as increase the duration of its storage, enzymatic hygiene 35 Drolysis of the substance t enzyme preparation Lysosubtilin GZH, having