SU1622388A1 - Method of producing feed protein - Google Patents

Abstract

The invention relates to biotechnology and relates to the production of feed protein biomass, which can be used in animal husbandry. The purpose of the invention is to expand the technological capabilities of the method. The method is fixed to the impelled strains of basal plains, of the fungus Coriohis hirsutjis IKK-158 or Carolina trogii Hl.h-3i7, million r.imis li | - Viniis I1Zh-131, or Daedal i a f | uercina ILK-350 by the solid phase method on block ni.iAiiMK.4x, the content of organic acids in which does not exceed 6 g / kg residue. Acid-lowering is carried out by chemical (adding chalk) min biochips (grown yeast strain Schi zosacch.iromyces aciJodevoratus 4-540 for 2 days at 4 ° C) by. After 10 days of cultivation of one of the proposed ptamop Oazitsn & Mitsetov, the final product contains up to 127 and 4.5% lnpids. 3 tab. with Ј C / s

Description

The invention relates to biotechnology and relates to the production of feed protein biomass, which can be used in animal husbandry.

The purpose of the invention is the expansion of technological capabilities.

The way is to follow.

The moistened raw material, which is used as block squeeze, is sterilized, the basidiomycelium mycelium is introduced into it, cultivated and dried, and the resulting biomass is dried. Before introducing the mycelium, a test seeding of it on samples of the raw material in which acid-reduction to various concentrations of organic acids is carried out is determined by condensation. A radioactive system in which active development of the fungal mycelium is ensured and heaping and lowering the raw material to this concentration is carried out. The optimum content of organic acid in the substrate is not higher than 6 g / kg. On the raw material previously prepared in this way. tivuyu strains of basidiomycetes fungi Cor.iolus hirsutus IBK-358 or Funalia

(X

wasp

316223

trogii IKB-337. or Panua tip.rinus IKB-131, or Paaeda quercina IKB-350. The connection between the possibility of cultivating the strains of baeidial fungi (the ability of their development) and the concentration of organic acids in the raw material was established for the first time. The limiting concentration of organic acids, to which JQ is economically most effective, but the application of the method is equal to 6 g / kg. The use of acid lowering by chemical or biological means is well known, for example, acid lowering of winematerials to enhance the organoleptic properties of wine.

Acid-lowering in the proposed method cannot be considered as establishing the optimum pH of the medium.

Different concentrations of organic acids and, after 1 day, visually determine the sample with the concentration of acids at which the active development of the mycelium is observed. Then chemical or biological acid reduction of the whole raw material to this concentration is carried out. The seed in the amount of 3% is mixed with raw materials, placed in chambers-cassettes with a capacity of up to 1 m at the rate of 0.5 tons per chamber, and solid-phase cultivation is carried out for 7-10 days. The obtained biomass is dried at 100-140 ° G, to a constant weight and packed in 30-50 kg each.

The most effective use as a source of feed protein

With a significant change in the concentration of - 20 strains of basdial fungi

Coriolus hirsutus IBK-35Ya, Funalia trogii IBK-337, Panus tigrinus IBK-131, Daedalia / fluercinia IBK-350 The process of bioconversion of raw materials by cultivating mushrooms on it is most effective when the content of organic acids is not more than 6 g / kg.

of organic acids in blocky husks, the pH value (Table 1), changes slightly.

Such a change in the pH value cannot have a significant effect on the growth of fungal strains, as evidenced by experiments to determine the pH values of block marc optimal for the development of basidiomycetes pH (Table 2). In these experiments, a pH of 2-10 is obtained by introducing caustic soda and hydrochloric acid into a sterile substrate and controlling the potentiometric. After chemical acid reduction to the optimum level of organic acids, basidiomycete fungi are cultivated, and the protein content is determined in the final feed product.

From Table 2 it follows that the pH range of 4-8 is optimal for the growth of basidiomycetes. The initial value of the pH of the block husk is 5 and, therefore, is included in this interval, so its artificial change using alkalis or acids is impractical.

The method is carried out as follows.

High-acidic waste products of juice, non-alcoholic or canned products, which under production conditions are stored in Kraft tags, are moistened with water with a hydronic module; 1.0-1.5-and sterilized (temperature 130 °, time 30 min).

After that, the mycelium of the basidio fungus is sown on Petri dishes filled with agar squeeze with

20 strains of basidi mystic fungi:

25

thirty

35

40

45

50

Coriolus hirsutus IBK-35Ya, Funalia trogii IBK-337, Panus tigrinus IBK-131, Daedalia / fluercinia IBK-350. The process of bioconversion of raw materials by cultivating mushrooms on it most effectively takes place when the content of organic acids is not more than 6 g / kg.

Example 1. The raw materials are dry block marc with a concentration of organic acids of 14 g / kg. The husks are moistened with a water ratio of 1.0 to a moisture content of 50%. Then, chemical drying of the marc is carried out to 6.0 g / kg by adding chalk at the rate of 30 g per 1 kg of the marc and sterilized in an autoclave (1 atm, 130 ° C, 30 min). Coriolus hirsutus IBK-358 mycelium seed seeds are introduced into the substrate prepared in this way.

Sowing material is prepared by transferring the mycelium from a test tube in which it is stored in the collection onto a Petri dish with an agarized beer wort and then subcultured on the husks placed in a glass jar at the rate of 1/4 Petri dish per 50 g husks. In the bank, the growth of the mycelium continues for 7 days until it is completely overgrown, which is established

visually.

one

The prepared seed is introduced into the husks at a ratio of 1:30 (by volume) and solid-phase cultivation is carried out at 10 ° C for 10 days. The resulting biomass is dried. The finished product contains

10.5% protein and 4.07 lipndop. The amino acid composition of the protein is attached in Table 3.

Example 2. Strain and culture conditions as in Example 1, but acid reduction is carried out until the organic acid content is 4.5 g / kg by adding chalk at the rate of 37.3 g / kg of residue. The resulting biomass contains 10.5% protein and 4.5% lipids.

Example 3. Strain and culture conditions as in Example 1, but acid lowering is carried out to an organic acid content of 7.0 g / kg by adding chalk at a rate of 20 g / kg of residue. The resulting biomass contains 17, proteins and 3.52 lnpids.

Example 4. The strain of the fungus and the cultivation conditions as in Example 1, but acid reduction was carried out biologically using the yeast strain Schizosaccharomyces acido-devoratus VKPM 4-450 as follows. From the dense nutrient medium, the yeast is subcultured into a test tube with a sterile block wort and, after vigorous clogging, are transferred into a flask with 0.5 l of sterile wort. Cultivation was carried out for 2 days with no aeration until rapid fermentation was achieved (1 ml contained 100-150 million cells, of which 45% were verse).

The prepared suspension of yeast is put into block marcine at the rate of 2%. After 2 days soak in

ten

Phase cultivation of Coriolus hirsutus strain IB-358 on the obtained substrate, the final product contains 127 proteins and 4% lnpnds.

Example 5. The cultivation conditions are as in Example 1, but the strain Funalia trogii IBC-337 is consumed. The final product contains 10.5% protein and 4% lipids.

Example 6: Cultivation conditions as in Example 1, but Pancis tigrimis strain IBC-131 is grown. The final product contains 10.57 protein 15 and 4% lipids.

Example 7. The cultivation conditions are as in Example 1, but the Daedalia quercina INC-350 strain is grown. The final product contains 12Z protein and 20 4.57 lipids.

Claims (1)

Invention Formula A method for producing feed protein, 25 which provides for the cultivation of fungal strains - sources of feed protein on a nutrient medium containing block marc as the main substrate, up to a maximum of 30 accumulations of the target product, which is characterized by the fact that, in order to expand the technological capabilities of the method, A strain is used as a source of feed protein. the fungus Coriolus hirsutus IBK-35I, or Funalia trop.ii IB-337, or Panus tigrinus IB-131, or Daedalia quercina IB-350, and the process is carried out by the method of solid-phase fermentation; the concentration of organic acid is 40% organic acid in the sublot in sludge to 6 g / kg After firmness, not exceeding 6 g / kg. thermostat at 24 ° C happens sn5, 0 Chalk (20 g / kg) 5.0 0.2% (MH +) GPO ". 5.0 27 (NH4) CP04 5.8 0.2% urea 4.6 Yeast-sugars at 2% 5.0 Phase cultivation of Coriolus hirsutus strain IB-358 on the obtained substrate, the final product contains 127 proteins and 4% lnpnds. Example 5. The cultivation conditions are as in Example 1, but the strain Funalia trogii IBC-337 is consumed. The final product contains 10.5% protein and 4% lipids. Example 6: Cultivation conditions as in Example 1, but Pancis tigrimis strain IBC-131 is grown. The final product contains 10.57 protein 5 and 4% lipids. Example 7. Cultivation conditions as in Example 1, but Daedalia quercina INC-350 are grown. The final product contains 12Z protein and 0 4.57 lipids. Invention Formula A method for producing feed protein, 5 involving the cultivation of fungal strains - sources of feed protein on a nutrient medium containing block marc as the main substrate, to the maximum accumulation of the target product, which is characterized by the fact that, in order to expand the technological capabilities of the method, A strain is used as a source of feed protein. the fungus Coriolus hirsutus IBK-35I, or Funalia trop.ii IBC-337, or Panus tigrinus IBC-131, or Daedalia quercina IBC-350, and the process is carried out by the method of solid phase fermentation at 0 ° C organic acids in a substrate not exceeding 6 g / kg. Table 1 14.0 7.0 18.1 38,8 14.0 7,8 4.5 4.5 4.8 7.210.5 10.510.3 6.5 4.5 4.5 Amino acid Asparagine Threonine Series Glutaminov Proline Glycine Alanya Valin Methionine Isoleucine Leucine Tyrosine Phenylalanine Histidine Lysine Arginine T a b l and c b 2 Table 3 Content in protein,% 1.80 0.65 0.95 2.85 0.25 M5 1.05 1.15 ABOUT 0.10 0.80 ABOUT 0.15 0.05 ABOUT 0.15