SU469748A1 - Device for monitoring the physiological state of cells - Google Patents

Description

one

The invention relates to a device for monitoring the physiological state of cells, widely used in scientific research and in the microbiological industry.

Devices are known for monitoring the physiological state of the cells, for example, by their behavior in a non-uniform electric field, containing a source of light, a dielectric cell with a cavity and electrodes, a photocell, a self-recording device and a high-frequency generator.

However, such devices do not provide accurate control.

The aim of the invention is to improve the accuracy and acceleration of the control.

For this, in the proposed device, the side walls of the cavity are made of optical glasses, and the electrodes are located in the plane of the optical glasses and are made in the form of gratings.

The proposed device completely distinguishes the effect of cell orientation and chaining from the effect of dielectrophoretic deposition of cells on electrodes, and, in addition, the sensitivity of the device increases as the active area of the electrodes on which the cell deposit forms increases.

FIG. 1 is a block diagram of the device described; in fig. 2 - dielectrophoretic cell along the optical beam; in fig. 3 is a section along A-A in FIG. 2

The device for monitoring the physiological state of the cells consists of a light source 1, a dielectrophoretic cell 2, a photocell 3, a parking squad, its device 4, and a high-frequency generator 5.

The dielectrophoretic cell 2 is a plate 6 in which there is a cavity B; the side walls of the latter are made of optical glasses 7. On plate 6, fittings 8 are fixed and channels are drilled a. In the plane of optical glasses 7, electrodes 9 and 10 are arranged, made in the form of gratings and connected to a high-frequency generator 5. The distance between the electrodes and the number of bands making up the electrodes are chosen such that a non-uniform electric field is established in the cavity B.

The device works as follows.

Cavity B is filled with a cell suspension using fittings 8 and channels a. The luminous flux from the light source 1, passed the dielectrophoretic cell 2, the photocell 3 is converted into an electrical signal recorded by the recorder 4

Immediately after switching on the generator 5, the cells in the volume of the suspension are oriented in an electric field, forming a chain, and moving in the direction of the maximum intensity of the electric field to electrodes 9 and 10, forming a deposit on them. In this case, the recorder 4 first registers a sharp, ending in a few seconds, a decrease in the luminous flux, and then a gradual increase in the luminous flux.

The physiological state of the cells is judged by both the recorded effects. The magnitude of the stepwise decrease in the transmitted light flux gives information about the process of cell orientation and the formation of chains,

and the subsequent gradual increase in the transmitted light flux is about the amount of sediment at the electrodes.

Prdmet invention

A device for monitoring the physiological state of cells, for example, by their behavior in a non-uniform electric field, containing a light source, a dielectric cell with a cavity and electrodes, a photocell, a self-recording device and a high-frequency generator, in order to increase accuracy and speed up control, lateral the walls of the cavity are made of optical glasses, and the electrodes are located in the plane of optical glasses and are made in the form of gratings.

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Fig .. 2

FIG. 3