SU457723A1 - Strain 313-152 actinomyces sp.as producer of uratoxydase - Google Patents

Description

I

The invention relates to the field of medicine-iyy, namely to the production of enzymes.

It is known to use Candida niltus yeast, BacUEus foisidiosus bacteria, and others as a producer of urotoxicity. 5

However, the known strains have low activity.

The aim of the invention is the use of the Ac-t strain 313 ...- 152. sp.

 Strain 313-152 AciitiOtnfces sp. - Protozo uretoksatsy. Stored in the collection of cultures of the All-Union Scientific Research Institute of Antibiotics (Moscow), Mg 1510.

To obtain a seed culture, the 3i3-l52Acli-tiotrtvce8 sp-strain was seeded on 15 canted agar containing QN PO. OD%, Mg-SO 0, O5; o, KSE 0.05%, FeSO% mg / ml, GriSOy 0.5 mg / ml, uric acid O, O259o, agar 2%, and are grown for 7 days at 28 C. The original strain is stored at 4 C iMi on oblique agar with oats broth /

Cultural-morphopological character.

On synthetic medium with glucose, whitish, mealy, substrate-25 mycelium mycelium

martial mycelium marble-pink, does not form soluble pigment. With growth on Gause1 # 2 medium, aerial mycelium is pale pink, substrate mycelium is terracotta, there is no pigment. There is no aerial mycelium on the medium of Gause N 2, substrate mycelium is bistral, the pigment is ano-brown-violet. When growing on oats, the aerial mycelium is white, the substrate mycelium is dark-colored, the pigment is gray-dark-purple. On the soy medium, the aerial mycelium is whitish, poorly developed, the substrate mycelium is beige, and there is no pigment. With growth on the pea environment, the aerial mycelium is white, poorly developed, the substrate mycelium is beige, and there is no pigment. Sporonosa straight, wavy, oval spores.

Physiological and biochemical properties ..

Claims (1)

Strain gitsrolizuet starch, gelatin thins slowly. With growth on medium with gelatin, a red-brown pigment is released. Milk peptonizes slowly, without prior | coagulation. i Melanoid pigment does not secrete. Uses glucose, mannitol, xylose, ramnoeu, does not consume oeite as the only carbon source, can use uric acid as the only source of nitrogen and uperode. Urotoxidase is formed inductively, i.e. only when lyrashivanii on environments with washing acid. The enzyme has intracellular localization and is not released into the culture medium. The strain is administered. For the penumbra of the enzyme, the seed culture is transferred into 750 ml flasks containing; aO ml of medium of the following composition: 0.1%. Mg-bO 0.5%, KCE 0.5X FeSO 5 mg / ml. C $ 0 0.5 mg / ml, uric acid 0.3-1.O%. water is water-water, pH 7.3. The cultivation of the actinomytet is carried out for 9-4 days with the shaker. In order to extract the enzyme, the destruction of the cells is carried out by ultrasound. With the destruction of the density of bacterial cycne: issm 0.5 g wet weight per ml and 0.1 M borate buffer pH 8.5. In the cell extract, the specific activity of the enzyme is O.1O-0.25 U / mg protein (determined by the Lowry method) or 0.4-0.8 U / ml of extract. Thus, based on 1 g wet weight of biomass, there are 8-12 E, and per 1 ton of dry biomass 32-48 E. The formula was invented and Strain 313-152 Actinotnyces sp. Is the origin of urate oxidase. Stored in the collection of cultures of the All-Union Scientific Research Institute of Antibiotics (Moscow) J 1510. Morphological features. In the synthetic medium with glucose, the aerial mycelium is whitish, the substrate mycelium is marble-pink and does not form a soluble pigment. On Gause No. 1 medium, aerial mycelium is pale pink, substrate mycelium is terracotta, there is no pigment. On the oat medium, the aerial mycelium is white, the substrate mycelium is dark brown, the pigment is gray-violet. In the soybean environment, the aerial mycelium is whitish, very poorly developed, the substrate mycelium is beige, Hei pigment. On the pea environment, the aerial mycelium is white, poorly developed, the substrate mycelium is beige, there is no pigment. There is no aerial mycelium on Gauze N 2 medium, cv6cTpaTHbiA mycelium is bistral, the pigment is dirty-brown-violet. Sporostsy pr "1) s. wavy, oval spores. Biochemical signs. Strain gilrolizuet starch gelatin thins slowly. When grown on medium with gelatin, it releases a red-brown pigment. Peptony milk slowly without .coaguy .dick. Melanoic pigment does not secrete. Physiological signs. The strain as the only carbon source uses glucose, mannitol, xylose, and rhamnose, does not use inositol. Uric acid can be used as the sole source of nitrogen. It also grows on uric acid as the sole source of both carbon and aaota.