SU452581A1 - The method of obtaining biomass - Google Patents

Description

The invention relates to the microbiological industry, namely to a method for the production of microbial biomass on pulp and paper production waste. Known method of obtaining biomass By isolating microorganisms under conditions of aeration in a nutrient medium containing sources of carbon, nitrogen and mineral salts, followed by release of biomass. In this case, the carbon source, cellulose, is preliminarily subjected to a guide: a rolyse, which leads to a complication of technology and a rise in the cost of the product. In order to be able to obtain biomass on pulp and paper production wastes, it was proposed to use active sludge as a source of microorganisms, and cellulose fiber as long as a carbon source with a length not exceeding 0.5 cultivation is recommended to lead at pH 4-6. Active sludge is a collection of bacteria, yeast and mold fungi, the simplest and most uncomplicated, developing in the wastewater facilities of pulp and paper mills. The fine cellulose fiber is a low molecular weight fraction of cellulose with a degree of polymerization of about 50 ° C, consisting mainly of fiber fibers with a length of no more than 0.5 mm and containing up to 80% A cellulose. The fine fiber contains up to 0.7% ash, including 3.02 mg / kg of manganese, 4O6 mg / kg of calcium, and 283.5 mg / kg of iron. Example. In a 25O ml conical flask containing 16 g of moist fine fiber (humidity about 90%), 0.5 g of activated sludge is added in the form of an aqueous suspension with a concentration of 10 g / l. 5 mg of nitrogen and 3 mg of phosphorus are added to the flask in the form of solutions of the nutrient salts of potassium phosphate and ammonium nitride. Within 6 days, biomass decomposition occurs at 25-ZO C and pH 5-6.

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Fiber decomposition is controlled by changing the degree of polymerization of cellulose (from the initial 540-5OO to the final 60 or less) and microscopic analyzes.

After 6 days, a layer of biomass appears. Chemical and microscopic analyzes show the complete absence of cellulose. The resulting biomass contains%: total nitrogen 7.6-10.0; phosphorus .1.4-2.6;

ash 9.5-12.0.

Vitamin content, mg / kg dry weight: A up to 28.0; C to 416; В 3, О-30, О.

The composition of biomass includes the whole complex of essential amino acids, as well as the enzyme cellulose.

Example 2. In a 15 l aeration vessel, 150 g of wet cellulose fine fiber (humidity 89%) are placed and 5 l of activated sludge (concentration 10 g / l), nutrient salts containing 24 mg of nitrogen and 12 mg of phosphorus are added. In a vessel pour up to 1O l of water. After one day, the mass was settled and 5 l of liquid was removed. Then up to 10 liters of water are added to the vessel and again the same as before, the amount

salts. After 5 days, aerobic decomposition of cellulose by activated sludge microflora is observed.

The resulting biomass contains nitrogen up to 1O%, phosphorus up to 2.5%, ashes up to 1O%, vitamins A, C and B, the whole complex NES-L.

amino acids.

Subject invention

The method of obtaining biomass by growing microorganisms under aeration conditions in a nutrient medium containing sources of carbon, nitrogen and mineral salts, followed by release of biomass, characterized in that the whole potential of obtaining biomass on pulp and paper production wastes. sludge, and as a source of carbon - cellulose fiber with a length of not more than 0.5 mm in a ratio of 0, while pulling is carried out at a pH of 4-6.