SU439780A1 - The method of obtaining a photographic image - Google Patents

Description

The invention relates to a method for producing photo-free photographic images.

Methods of obtaining photographic images by exposing the substrate of the photosensitive substance and substrate, exposing, developing the latent image and fixing the visible image using photosensitive substances such as diao compounds, photosensitive dyes, complex iron compounds, alkali metal dichromates, etc. are known.

The disadvantage of such methods of obtaining photographic images is that the materials used in sensitivity are noticeably inferior to silver sulfide layers, which requires high-intensity sources for their copying and often does not allow for obtaining good quality images.

In connection with an increase in the deficit of silver, there is a need both in the improvement of well-known photographic techniques and in the development of new methods for obtaining unserized photographic images.

The aim of the invention is a method for obtaining non-silver optical heating images using materials that are highly sensitive to ultraviolet radiation of moderate intensity and photosensitivity approaching halogen-silver materials.

Non-silver photographic images can be obtained by applying a photosensitive catalyst (nairimer, g-cynimoyl derivative of a proteolytic enzyme) to the substrate) and a substrate (for example, β-acyl l-amino-acid p-naphthylether), exposing the photosensitive material with UV light (wavelength 260 360 nm) by amplifying the resulting latent image by wetting and holding the material in

a medium with a pH of 8.0, developed by treating a diazo compound with a salt solution, in which the reaction between the p-naphthol group and a diazo compound proceeds, and fixing the image by treating the material with a denaturing protein agent.

The proposed method of a non-cerebral highly sensitive photograph is based on the principle of catalytic enhancement of a latent photographic image due to a chemical process using known catalysts modified so that their catalytic activity can be regulated with a beam of light. Obtaining a latent image is based on the exposure of active catalysts to light (in particular, biological catalysts — enzymes). In the absence of free diffusion of atalizatar (the catalyst is deposited on the substrate), the exposed areas of the light are different from those not exposed to the presence of a catalyst capable of participating in a chemical reaction.

During the subsequent treatment of the eco-material with reagents that undergo chemical transformation under the action of the catalyst, the reaction products accumulate on the exposed areas of the layer, while the non-copied areas do not change. Thus, the latent chemical change caused by light (photoactivation of the catalyst) is intensified by the conjugated chemical process. In the event that the reaction products are dyes or substances that can be easily transformed into dyes adsorbing on the substrate, a visible photophographic image can be obtained. As light-activated catalysts that are sensitive to ultraviolet light, it is possible to use proteolytic enzymes modified by a cyscinamoyl group at the active center, which can be converted into active catalysts by the action of light. The process of photochemical activation of g (c-cinnamoyl-chymotrypsin), the subsequent hydrolysis of the compound beluchromophor, is known to be a model of the mechanism of the primary processes of visual arousal.

Photosensitive materials are obtained by depositing a substrate and a photosensitive catalyst on a substrate. The substrate used is p-naphthyl ester of N-acylated aliphatic amino acid, or p-naphthyl ester of L-acetyl-b-phenyl-Nina. Substrates receive by the method described earlier. The preparation of a photosensitive catalyst (cc1Cl1nnamoylhi1Motrypsin) by the reaction of an enzyme with g of iscinnamoylimidazole is described earlier.

The resulting photosensitive material is sensitive only to ultraviolet light. The primary photochemical process in the ekoshanirovaniyu photosensitive layer is the photochemical reaction of the cystrans-isomerization of the cinnamoyl group of the modifier (H1NOGO from an ator.

The photosensitive paper based on ciscinnamoyl-a-chymotrypsin has a sensitivity range from 340 to 250 nm, based on 4nitro-cinnamoyl-a-chymotrypsin from 370 to 280 nm. The quantum yield of the primary process of isomerization of cinnamoyl-ss-chymotrilcine when illuminated by an aqueous solution of the acyl-enzyme with ultraviolet light of the specified wavelength range is 0.4

To obtain a latent image, the photosensitive material is exposed for a time from 0.01 to several seconds when using monochromatic light.

lamly DRSh-260 with intensity (at nm) 5-10 kV / cm-sec. More exposed areas of the photosensitive material have a higher concentration of t / 7ans-cinnamoylfermeite.

Enhancement of the latent image is based on the preparation of the active catalyst from the photo-formed trans-cinnamoyl enzyme and catalytic hydrolysis of the substrate by the released enzyme. Gras-cinnamoyl-a-chymotrypsin at a pH optimal for the catalytic action of the enzyme is a highly labile compound that undergoes hydrolysis to form cinnamic acid and is catalytically active | PO enzyme.

The half-turn of this reaction is approximately 1 minute. Dys-cinnamoyl-ahi Motipsin is a catalytically inactive stable compound.

In the presence of substrate and water, the enzyme formed from t / s of ans-cinnamoyl-a-chi1motripsin catalyzes the hydrolysis of the substrate, which leads to the accumulation of hydrolysis products. For each absorbed quantum of light in one second of the amplification reaction, due to the high catalytic activity of the catalyst, it is important that the molecules of the hydrolysis products develop. This allows the primary latent image to be enhanced.

To this end, the exionated photosensitive material is kept in a wet state at a pH optimal for the catalytic activity of the enzyme. At this time, the transacyl enzyme deacylation and substrate hydrolysis take place under the action of an extruded catalyst.

The appearance of the latent image enhanced by a chemical reaction is based on the conversion of the products of the reinforcing chemical reaction into dyes. When using the N-acetyl-b-amino acid p-naphthyl esters, the hydrolysis products are || 3-naphthol and an acylated amino acid. Paftol can be converted into azo dyes by reaction with salts of diazo compounds. For this purpose, the photographic material after the enhancement process of the latent image is treated with a solution of a diazonium syrup.

As a result of the reaction of p-naphthol with the dia-salt, a dye is formed, which turns the enhanced latent image into a visible one. Depending on the structure of the salt of the diazo compound used, various colorants can be obtained, which makes it possible to obtain images of different colors.

. The color of the resulting

The amine used to represent the preparation of the diazo compound

Yellow

Orange

Orange red

Red

Purple

in

The image fixing process is based on the destruction of the catalytic activity of the photo-treated catalyst. For this, a photographic image is treated with a solution of protein-denaturing agents, for example, 1M trichloroacetic acid or 8M urea. Processing such reagents destroys the tertiary structure of the protein, which leads to the loss of the enzyme activity. After fixing, the material is dried at 80-100 ° C.

An example of obtaining a photographic image.

The photosensitive material is prepared by impregnating a sheet of white paper with M-acetyl-L-phenylalanine p-naphthyl ether solution in acetone (concentration of I-IO mol / l), drying and registering with a water solution of cns-cinnamoyl-a-chymotrypsin (1-10 mol / l of g {ys-cinnamoyl-a-chymotrypsin, 1-10 mol / l HCl) and drying at room temperature. Photosensitive paper is exposed for 1 sec. With ultraviolet light of a DRSh-250 mercury lamp at a distance of 30 cm from the light source. Impregnated paper is impregnated with a solution (0.1 mol / l of KH2PO4) with a pH of 8.0, kept in a wet state for 15 minutes, treated with a solution of p-anispdipdiazonium chloride (1-10-3 mol / l, pH 8.0, 0.1 mol / l KH2P04), incubated for 10 minutes Then the paper is impregnated with a solution of urea (8.0 mol / l) and dried at 80-100 ° C. Get a visible photographic image.

The proposed fabricated material differs from other non-halogen-silver photosensitive materials (diazo type, etc.) by increased photosensitivity.

In terms of photosensitivity, the material predisposed is superior to diazo-type material and approaches the halo-sulfur and m materials. Contrast Ratio 0.7-0.9, resolution 80-200 lin / nm. The photosensitive can be used to take photographic images using ultraviolet rays of low intensity.

In addition to the p-naphthyl esters, you can use other esters of phenols KaiK benzene, and naphthalene series (phenol and its derivatives, naphthol and its peroxides, water) and aromatic or aliphatic M-acyl-L-amino acids.

Subject Iso-br & Shadow

The method of obtaining a photographic image by applying a photosensitive substance and a substrate to the substrate, exposing, enhancing and obtaining an open image, manifesting and fixing, characterized in that, in order to obtain a photorebrisive photographic image using low-intensity ultraviolet radiation, as a photosensitive substance are ciscipmunoyl-derived proteolytic

enzyme and as substrate - aryl

N-acylated L-amino acid ester, photosensitive material is exposed to ultraviolet light with a wavelength of 260-360 nm, the resulting latent image is enhanced by moistening and holding the material in a medium of pH 8.0, the enhanced latent image is developed by treatment with a diazo compound salt solution and the visible image is fixed by treating the material with a protein denaturing agent.