SU399770A1 - METHOD OF MEASURING THE MEDIUM LIFE-TIME OF EXCITED LIGHT FLOW MOLECULES IN FLUORESCENCE - Google Patents
METHOD OF MEASURING THE MEDIUM LIFE-TIME OF EXCITED LIGHT FLOW MOLECULES IN FLUORESCENCEInfo
- Publication number
- SU399770A1 SU399770A1 SU1499259A SU1499259A SU399770A1 SU 399770 A1 SU399770 A1 SU 399770A1 SU 1499259 A SU1499259 A SU 1499259A SU 1499259 A SU1499259 A SU 1499259A SU 399770 A1 SU399770 A1 SU 399770A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- fluorescence
- measuring
- time
- excited light
- light flow
- Prior art date
Links
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Description
1one
Изобретение относитс к области технической физики и может быть использовано во всех случа х, когда требуетс измерение среднего времени жизни возбужденных молекул при флуоресценции.The invention relates to the field of technical physics and can be used in all cases where the measurement of the average lifetime of excited molecules during fluorescence is required.
Известен способ определени среднего времени жизни возбужденных молекул, в котором производ тс последовательно измерени кеэффициентов модул ции возбуждающего светового потока и светового потока флуоресценции посредством ФЭУ, резонансного усилител , настроенного на определенную частоту модул ции с детектором дл определени переменных составл ющих световых потоков, а затем вычисл етс среднее врем жизни возбужденных молекул.There is a method for determining the average lifetime of excited molecules, in which the modulation coefficients of the excitation light flux and fluorescence light flux are measured successively by a photomultiplier tube, a resonant amplifier tuned to a specific modulation frequency with a detector to determine the variable components of the light fluxes, and then calculate The average lifetime of the excited molecules.
Однако необходимость производить в различное врем две пары измерений: измерение среднего значени возбуждающего светового потока и его переменной составл ющей на нефлуоресцирующем объекте и те же измерени светового потока на исследуемом объекте, делает невозможным использование прибора в качестве регистрирующего дл непрерывного исследовани изменени среднего времени жизни возбужденных молекул в процессе, например , химической реакции.However, the need to perform two pairs of measurements at different times: measuring the average value of the exciting light flux and its variable component on the non-fluorescent object and the same measurements of the light flux on the object under study makes it impossible to use the instrument as a recording for continuous study of the change in the average lifetime of the excited molecules in a process, for example, a chemical reaction.
Целью изобретени вл етс повыщение точности измерений и получение возможностиThe aim of the invention is to increase the accuracy of measurements and to obtain the possibility
непрерывной регистрации в процессе, например , химической реакции.continuous registration in the process, for example, a chemical reaction.
Это достигаетс тем, что световой поток поочередно модулируют по синусоидальномуThis is achieved by alternately modulating the luminous flux of
закону двум сильно разнесенными частотами , а затем измер ют разность интенсивности переменных составл ющих света флуоресценции на обеих частотах модул ции и вычисл ют среднее врем жизни возбужденных молекул .law, two highly separated frequencies, and then the difference in the intensity of the variable fluorescence light components at both modulation frequencies is measured and the average lifetime of the excited molecules is calculated.
Описываемый способ позволит определ ть врем жизни возбужденных молекул пор дка сек, тогда как измерение по известному способу не дает возможности определить врем жизни менее Ш сек. . Кроме того, можно исключить базовый объект и регистрировать непрерывно врем жизни возбужденных молекул в ходе какого-либо процесса , например, химической реакции.The described method will make it possible to determine the life time of excited molecules of the order of seconds, while the measurement by a known method makes it impossible to determine a life time of less than W sec. . In addition, it is possible to exclude the base object and record the lifetime of the excited molecules continuously during some process, for example, a chemical reaction.
Предмет изобретени Subject invention
Способ измерени среднего времени жизни возбулчденных световым потоком молекул при флуоресценции, отличающийс тем, что, с целью повышени точности измерени и получени возможности непрерывной регистрации в процессе, например, химической реакции, световой поток модулируют двум разнесенны3 ми частотами по синусоидальному закону, а затем измер ют разность интенсивности неременных составл ющих света флуоресцен4 ции на обеих частотах модул ции и вычисл ют среднее врем жизни возбужденных молекул .The method of measuring the average lifetime of molecules excited by the luminous flux under fluorescence, characterized in that, in order to increase the accuracy of measurement and to obtain the possibility of continuous recording in a process, for example, a chemical reaction, the luminous flux is modulated with two separated frequencies sinusoidally and then measured the difference in intensity of the time-dependent fluorescence light components at both modulation frequencies and the average lifetime of the excited molecules is calculated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1499259A SU399770A1 (en) | 1970-12-14 | 1970-12-14 | METHOD OF MEASURING THE MEDIUM LIFE-TIME OF EXCITED LIGHT FLOW MOLECULES IN FLUORESCENCE |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1499259A SU399770A1 (en) | 1970-12-14 | 1970-12-14 | METHOD OF MEASURING THE MEDIUM LIFE-TIME OF EXCITED LIGHT FLOW MOLECULES IN FLUORESCENCE |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU904887264A Addition SU1749843A2 (en) | 1990-11-29 | 1990-11-29 | Device for measuring average rate of frequency and linearity of modulation characteristics of frequency-modulated generators |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU399770A1 true SU399770A1 (en) | 1973-10-03 |
Family
ID=20461065
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU1499259A SU399770A1 (en) | 1970-12-14 | 1970-12-14 | METHOD OF MEASURING THE MEDIUM LIFE-TIME OF EXCITED LIGHT FLOW MOLECULES IN FLUORESCENCE |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU399770A1 (en) |
-
1970
- 1970-12-14 SU SU1499259A patent/SU399770A1/en active
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