SU391172A1 - METHOD FOR DETECTING NUCLEIC ACIDS L - Google Patents
METHOD FOR DETECTING NUCLEIC ACIDS LInfo
- Publication number
- SU391172A1 SU391172A1 SU1706662A SU1706662A SU391172A1 SU 391172 A1 SU391172 A1 SU 391172A1 SU 1706662 A SU1706662 A SU 1706662A SU 1706662 A SU1706662 A SU 1706662A SU 391172 A1 SU391172 A1 SU 391172A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- nucleic acids
- detecting nucleic
- rifadin
- cells
- preparations
- Prior art date
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
1one
Изобретение относитс к области медицины .This invention relates to the field of medicine.
Известен снособ вы влени нуклеиновых кнслот путем индукции люминесцентного свечени обработкой клеток акридин-оранжем. Этот способ, однако, характеризуетс длительностью процесса.A known procedure for detecting nucleic acids by inducing luminescence is effected by treating cells with acridine orange. This method, however, is characterized by the duration of the process.
Дл упрощени процесса в предлагаемом способе в качестве индуктора используют рифа д и н (риф а м пи ци;н).To simplify the process, in the proposed method, a reef d and n (reef am pi pi; n) is used as an inductor.
Способ не требует предварительных условий произведени реакции, и не зависит от спирализации нуклеиновых кислот в клетках. Дл вы влени нуклеиновых кислот в клетках фиксированные препараты обрабатывают в растворе рифадина (рифампицина) 1 : 1000- 1 : 10000 в цитратно-фосфатном буфере при рН 4,2-4,5 в течение 5-30 мин, затем препараты промывают проточной водой 5 мин, высушввают на воздухе и заливают нефлуоресцируюидим вазелиновым маслом. Исследование препаратов провод т под люминесцентным микроскопом в фиолетово-синей-части спектра. К летки, обработанные рифадином (ри.фампицином ) дают флуоресценцию нуклеиновых кислот в зеленой области спектра. После удалени нуклеиновых кислот ферментами или хлорной кислотой, флуоресценци исчезает, заметно лишь слабое свечение фона и контуров клетки.The method does not require preliminary conditions for the reaction, and does not depend on the spiralization of nucleic acids in cells. To detect nucleic acids in cells, fixed preparations are treated in a solution of rifadin (rifampicin) 1: 1000-1: 10000 in citrate-phosphate buffer at a pH of 4.2-4.5 for 5-30 minutes, then the preparations are washed with running water 5 minutes, dried in the air and pour non-fluorescently petroleum jelly. The study of the preparations was carried out under a fluorescent microscope in the violet-blue part of the spectrum. By the year-rounds, treated with rifadin (ri.fampicin) give a fluorescence of nucleic acids in the green region of the spectrum. After the nucleic acids are removed by enzymes or perchloric acid, the fluorescence disappears, only a faint glow of the background and cell contours is noticeable.
Предмет изобретени Subject invention
Способ вы влени нуклеиновых кислот путем обработки клеток индуктором люминесцентного свечени , отличающийс тем, что, с целью упрощени процесса, в качестве индуктора используют рифадин (рифамницип).The method of detecting nucleic acids by treating cells with a fluorescent luminescence inducer, characterized in that, in order to simplify the process, rifadin (rifamun) is used as an inducer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1706662A SU391172A1 (en) | 1971-10-18 | 1971-10-18 | METHOD FOR DETECTING NUCLEIC ACIDS L |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1706662A SU391172A1 (en) | 1971-10-18 | 1971-10-18 | METHOD FOR DETECTING NUCLEIC ACIDS L |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU391172A1 true SU391172A1 (en) | 1973-07-25 |
Family
ID=20490687
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU1706662A SU391172A1 (en) | 1971-10-18 | 1971-10-18 | METHOD FOR DETECTING NUCLEIC ACIDS L |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU391172A1 (en) |
-
1971
- 1971-10-18 SU SU1706662A patent/SU391172A1/en active
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