SU176851A1 - METHOD OF CULTIVATION OF MICROORGANIZL10V - Google Patents
METHOD OF CULTIVATION OF MICROORGANIZL10VInfo
- Publication number
- SU176851A1 SU176851A1 SU926280A SU926280A SU176851A1 SU 176851 A1 SU176851 A1 SU 176851A1 SU 926280 A SU926280 A SU 926280A SU 926280 A SU926280 A SU 926280A SU 176851 A1 SU176851 A1 SU 176851A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- nutrient medium
- granulated
- cultivation
- enzymes
- microorganisms
- Prior art date
Links
- 235000015097 nutrients Nutrition 0.000 description 6
- 238000005273 aeration Methods 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 241000228212 Aspergillus Species 0.000 description 1
- 240000007842 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 240000008529 Triticum aestivum Species 0.000 description 1
- 230000002478 diastatic Effects 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 210000004215 spores Anatomy 0.000 description 1
- 235000021307 wheat Nutrition 0.000 description 1
Description
Известны сиособы выращивани микроорганизмов , например илееневых грибков-продуцентов ферментов, путем посева их на CTepiiлизованпую и увлажненную нитательиую среду при иепрерывной ее аэрации. Так, известен 5 способ выращивани плесневого гриба Aspergillus aryzae в качестве продуцента протеолнтическнх и диастатическнх ферментов на предварительно спорилизованном и увлажненном белоксодержащем питательном сырье, на- 10 пример соевых зернах, соеволг шроте, жмыхе, при непрерывной аэрации. Предложено питательную среду предварительно гранулировать, что улучшает услови аэрации.15 Способ заключаетс в следующем. Питательную среду, соевый шрст или жмых раздробл ют в крупку, стерилизуют острым паром при 118-120-С, охлаждают, носле чего смешивают со стерильной ншеничной мукой. 20 олученную массу увлал и ют и гранулируют в крупные гранулы. Затем на массу засевают споры плесневых грибков, например . spergillus aryzae, которую помещают в аппарат дл проращивани грнба. Дальнейший процесс ведут согласно известным приемам. Гранулировать стерпльн ю питательную среду, засевать микроорганизмы на нее можно од ювременно i одном аппарате. Предмет изобретени Снособ выращивани микроорганизмов, иаиример плесневых грибков - продуцентов ферментов , путем их посева па стерилизованную н увлажненную ннтательную среду, при непрерывной аэрации ее, отличающийс тем, что, с целью улучшенн условий аэрацнн, питательную среду предварительно граиулируют.There are known methods for the cultivation of microorganisms, for example, ileeneic fungi-producers of enzymes, by sowing them on CTepilized and moistened nitrite medium during its continuous aeration. Thus, there is a known 5 method for growing molds of the fungus Aspergillus aryzae as a producer of protein and diastatic enzymes on previously sporulated and moistened protein-containing nutrient raw materials, for example, soybean, soyvolg meal, meal, with continuous aeration. The nutrient medium is proposed to be pre-granulated, which improves aeration conditions. 15 The method is as follows. The nutrient medium, soybean meal, or oilcake is crushed into a grit, sterilized with live steam at 118-120-C, cooled, and mixed with sterile wheat flour. The resulting mass is deposited and squeezed and granulated into large granules. Then, spores of mold fungi are seeded onto the mass, for example. spergillus aryzae, which is placed in a uvba germination apparatus. The further process is carried out according to known techniques. A sterile nutrient medium can be granulated; microorganisms can be seeded on it at the same time with one apparatus. The subject of the invention is the method of cultivating microorganisms, iairimer of mold fungi - producers of enzymes, by sowing them in a sterilized and humidified nutrient medium, with its continuous aeration, characterized in that, in order to improve the aeration conditions, the nutrient medium is pre-granulated.
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU176851A1 true SU176851A1 (en) |
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