SU1750492A1 - Method for growing grafted plants - Google Patents

Abstract

Use: reproduction of the improved planting material, early diagnosis of compatibility of the solder-rootstock combinations. SUMMARY OF THE INVENTION: Sterilization of a soil substrate for propagation of plants, sterilized directly in a cultivation vessel. Grafting of the propagated microculture is carried out on the microculture of the stock after planting and rooting the latter. 1 dw., 2 tab.

Description

The invention relates to agriculture, in particular, to plant growing, and can be used in the nursery for obtaining and propagating healthy planting material, as well as for early diagnostics of the compatibility of the graft-rootstock combinations.

There is a known method of grafting plants under sterile conditions using copulation, in which 4to the contact area of oblique cuts is fixed with a length of tube made of a polymeric material, then the grafted stock is placed in a sterile culture vessel with artificial nutrient medium, and after splicing the graft components the plants are planted in the sterilized soil substrate and the plants gradually adapt to non-sterile conditions.

There is a known method of growing grafted plants, according to which the graft material is propagated under sterile conditions, and a stem from a one-year matured shoot,

From the vegetative plant, the graft is placed in the split on the previously prepared cutting of the stock, after which the microvivac place is covered with a cover made of transparent polymeric material, and the basal part of the rootstock is treated with auxin, the rootstock is rooted and the graft is grown to the standard size in the greenhouse.

The closest technical solution known is the method of growing grafted plants using the tissue culture method, which consists in growing in vitro with a sunflower seedling, removing them from cultivation vessels, grafting the graft into an inverted T-shaped incision planting the resulting plant under sterile conditions on an artificial nutrient medium for splicing the grafting components, further transplanting it into a sterile soil substrate and gradually adapting and plants to non-sterile conditions.

I

ABOUT

four

Che

Yu

The disadvantages of the prototype method are the low yield of grafted material due to poor survival of the grafts, the complexity of the process is due to the difficulty of coupling the grafting components, as well as the long period of cultivation of grafted plants due to the large number of manufacturing operations.

The aim of the invention is to increase the yield of grafted plants during the cultivation of a healthy planting material, reduce the labor intensity of work and shorten the period of cultivation of plants by combining and reducing the number of technological operations.

This is achieved in that according to the proposed method of growing grafted plants, the substrate is prepared, the cultivation vessel is filled with it, the latter is covered with foil, the substrate is sterilized directly in the cultivation vessel, after which an opening is made in the coating of the cultivation vessel. and after it is rooted under sterile conditions, it is inoculated. The grafting was carried out without removing the stock from the culture vessel.

The proposed method differs from the prototype in that the rooting of the micro-root is carried out in a cultivation vessel containing a sterile substrate, insulated with foil from the external environment to prevent moisture loss and infection by pathogenic microflora, as well as the possibility of grafting rooted rootstocks without removing them from substrate, which completely eliminates the loss of stock material.

The drawing shows a cultivation vessel with a grafted plant growing in it.

For carrying out the method, a cultivation vessel 1 is prepared, for example a can or a glass with a capacity of 150-200 ml of heat-resistant material. Substrate 2 is prepared from peat, sand and perlite in a 1: 1: 1 ratio, moistened, filled with a cultivation vessel 1, closed with foil 3, and autoclaved for 1 h at a pressure of 2 atm. The cultivation vessel 1 thus processed with the soil substrate 2 is transferred to a laminar box, where in foil 3 an opening 4 is made with the aid of sewing, through which the microcultures of plant 5 taken from the plant tissue culture are planted. The microcurtain substrate 5 is pretreated with auxin by soaking in solution or dipping in powder or paste. Next, a transparent bottom coating vessel 6 of the same size is installed upside down on a cultivation vessel 1 with a micro-sided plant 5 planted in it to prevent the micro-sided plant 5 from drying out and seal the contact points of the vessels with an elastic material 7, for example an adhesive tape. After 3 weeks, the coating vessel 6 under the conditions of the laminar box is removed, the growth point of the rooted microtrache planter 5 is removed and the graft 8 micrograft is grafted in the split, after which the coating vessel 6 is again installed on the cultivation vessel 1 and the contact points sealed. After 2 weeks sealing

partially or completely destroy, thus providing adaptation of the plant to low air humidity, and after 1 week the coating vessel b is removed and a plant suitable for transplanting into open ground is obtained. To ensure 100% survival of plants in open ground, they are planted together with part of the soil substrate 2.

The use of the invention allows

to accelerate the production of plants of difficult-to-root varieties for 3-5 months, to increase the survival rate of vaccines by 30-40%, to reduce the material and labor costs for obtaining a healthy planting material of fruit breeds and their varieties (see Table 1 and 2).

Claims (1)

The invention of the method of growing grafted plants, including the preparation of the rootstock by growing the latter under tissue culture conditions, preparing the lead by growing under tissue culture conditions, grafting the micrograft into the decapitated stock, growing the grafted plant under sterile conditions until the grafting of the grafting components, subsequent transplanting into non-sterile conditions, characterized in that , in order to increase the yield of grafted plants, reduce the laboriousness of the process and reduce the time of growing grafted plants, sterilization of the soil substrate is carried out directly to the vessel in which then the microculture of the rootstock is rooted, and the micrograf of the graft to the stock is rooted onto the rooted stock without removing the latter from the cultivation vessel. Table 1 Ukrainian plum shoots during cultivation in vitro,%, ) Duration of passage 1 month. ... - no shoots suitable for rooting. Yield, grafted material on easily rooted grades,% table 2