SU1725109A1 - Способ жидкостно-хроматографического определени пол рных органических веществ - Google Patents
Способ жидкостно-хроматографического определени пол рных органических веществ Download PDFInfo
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- SU1725109A1 SU1725109A1 SU894745378A SU4745378A SU1725109A1 SU 1725109 A1 SU1725109 A1 SU 1725109A1 SU 894745378 A SU894745378 A SU 894745378A SU 4745378 A SU4745378 A SU 4745378A SU 1725109 A1 SU1725109 A1 SU 1725109A1
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- USSR - Soviet Union
- Prior art keywords
- determination
- polar organic
- liquid chromatography
- organic compounds
- chromatography method
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Description
ровани 1,0 мл/мин. Отдельные компоненты обнаруживают детектированием при длине волны 278 нм. Масштаб регистрации 1:10, врем движени ленты самописца 0,5 см/мин. Врем удерживани п-тирозола 18,0 мин, салидрозида 22,3 мин.
Дл нахождени калибровочных коэффициентов салидрозида и п-тирозола готов т смеси с известным содержанием этих веществ 0,01 - 1,0 мг/мл.
П р и м е р 2. Навеску корневищ родиолы Кириллова 1,0 г экстрагируют в колбе объемом 100 мл в количестве 15 мл при кип чении 15 мин. Экстракт упаривают под вакуумом при 40 - 45°С, раствор ют остаток в 20 мл воды, фильтруют и пробу жидкости 20 мкл ввод т в колонку, элюируют 3%-ным этанолом и 0,002 М раствором фосфата аммони .
Пример 3.1,0 г измельченных корне- вищ родиолы четырехлепестковой экстраги
0
5
руют водой 3 раза и дальнейшую обработку ведут как указано. В качестве элюента используют 4%-ный этанол с фосфатом аммони .
Claims (1)
- Формула изобретени Способ жидкостно-хроматографическо- го определени пол рных органических веществ , заключающийс в разделении пробы на Cie-функциональном сорбенте в потоке элюента с добавками и УФ-детектировании, отличающийс тем, что, с целью обеспечени возможности отделени салидрозида и п-тирозола в экстракте родиолы розовой, ускорени времени и сокращени трудоемкости разделени , в качестве элюента используют 2 - 5%-ный раствор этанола в воде с добавкой однозамещенного фосфата аммони , а УФ- детектирование компонентов провод т при длине волны 278 н/м.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU894745378A SU1725109A1 (ru) | 1989-10-03 | 1989-10-03 | Способ жидкостно-хроматографического определени пол рных органических веществ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU894745378A SU1725109A1 (ru) | 1989-10-03 | 1989-10-03 | Способ жидкостно-хроматографического определени пол рных органических веществ |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1725109A1 true SU1725109A1 (ru) | 1992-04-07 |
Family
ID=21472686
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU894745378A SU1725109A1 (ru) | 1989-10-03 | 1989-10-03 | Способ жидкостно-хроматографического определени пол рных органических веществ |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1725109A1 (ru) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6905706B2 (en) * | 2002-04-16 | 2005-06-14 | Swedish Herbal Institute | Medicinal herbal extract carpediol for treating depression |
| CN103012512A (zh) * | 2013-01-16 | 2013-04-03 | 河北科技大学 | 天然红景天中红景天甙的分离纯化方法 |
| CN107238671A (zh) * | 2017-08-09 | 2017-10-10 | 吉林省现代中药工程研究中心有限公司 | 一种贞芪扶正制剂中多种成分的含量测定方法 |
| CN109001310A (zh) * | 2018-06-27 | 2018-12-14 | 江苏康缘药业股份有限公司 | 一种大株红景天的检测方法 |
| CN109115910A (zh) * | 2018-09-27 | 2019-01-01 | 新乡医学院三全学院 | 一种同时测定不同产地女贞子中红景天苷、女贞苷、特女贞苷的方法 |
-
1989
- 1989-10-03 SU SU894745378A patent/SU1725109A1/ru active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6905706B2 (en) * | 2002-04-16 | 2005-06-14 | Swedish Herbal Institute | Medicinal herbal extract carpediol for treating depression |
| CN103012512A (zh) * | 2013-01-16 | 2013-04-03 | 河北科技大学 | 天然红景天中红景天甙的分离纯化方法 |
| CN103012512B (zh) * | 2013-01-16 | 2016-03-30 | 河北科技大学 | 天然红景天中红景天甙的分离纯化方法 |
| CN107238671A (zh) * | 2017-08-09 | 2017-10-10 | 吉林省现代中药工程研究中心有限公司 | 一种贞芪扶正制剂中多种成分的含量测定方法 |
| CN107238671B (zh) * | 2017-08-09 | 2020-07-31 | 吉林省现代中药工程研究中心有限公司 | 一种贞芪扶正制剂中多种成分的含量测定方法 |
| CN109001310A (zh) * | 2018-06-27 | 2018-12-14 | 江苏康缘药业股份有限公司 | 一种大株红景天的检测方法 |
| CN109115910A (zh) * | 2018-09-27 | 2019-01-01 | 新乡医学院三全学院 | 一种同时测定不同产地女贞子中红景天苷、女贞苷、特女贞苷的方法 |
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