SU1712408A1 - Nutrient medium for the preparation of clostridium sporogenes spores - Google Patents

Abstract

The invention relates to biotechnology, namely, to obtaining a spore culture test to control the sterilization regime in the fish canning industry. The purpose of the invention is to increase the yield of spores with HIGH heat resistance. The composition of the medium includes corn extract, yeast, thioglycolic acid, hydrolyzate casein, hydrolyzed meat, and water. The nutrient medium will allow an increase in the yield of spores to 10 cells / cm. ^ of the invention — an increase in the yield of spores with high thermal stability. Example 1: Clostrldlum-sporogenes culture 25 is cultured on nutrient media containing meat hydrolyzate, casein hydrolyzate or their mixture; The inhibition rate of sporulation on days 21–28 The hydrolyzate of ca and casein is mixed in equal amounts (for amino-ammonia nitrogen). The effect of the concentration of amino-ammoniac nitrogen in the nutrient medium on the sporulation is presented in Table 1. A culture of Clostridium sporogenes is sown on nutrient media containing different ratios of casein hydrolyzate, meat hydrolyzate, fodder yeast, corn extract, and thioglycolic acid. After the cultivation process has elapsed on days 21- ^ 28. take into account the number of spores formed. The results are presented in table 2. The resulting spores contaminate the batch of canned "Squid natural". The product is heated. The heat resistance of the spores is maintained at a high level of 1D121. 1 ° C, 0.72 minutes; Thus, spores CJ. sporogenes. obtained on casein-and-whey environment, have high thermal stability and can be used in industry for testing and developing canned sterilization regimes. water, characterized in that, in order to increase the yield of spores with high thermal stability, as a source of nitrogen C / 1Cioc > &

Description

it contains pancreatic hydrolyzed meat and casein hydrolyzed dry in the following ratio of components, g / l: dry casein hydrolyzed 16.5-18.5

Pancreatic Hydrolyzate MASA (200-10%

Nutrient medium

Types of Hydrolyzate of Am Ammonium Ammonium Nitrogen Amount per 130 170 200 250 130 Crop Growth Intensity Amount of Thermal Stability No: 10 10: 10 No Dispute Out 1 cm3 Results

amino-ammoniac

nitrogen)

Nutrient Yeast

Thioglycolic Corn Extract

acid

Water

Table 1

hydrolysis;

Casein Hydrolyzate

A mixture of hydrolysates of maca and casein + -H- + sporoobrazovanie on a nutrient medium with different ratios of the components included in it 170,220,250,130,220,220,250 + 4+ ++ "-t-H- + + -H- + -H. No 10 1H. / sJ 1,5icf -Ta B LI tsa 2

Claims (1)

Claim Nutrient medium for producing spores of Clostridium sporogenes, including fodder yeast, corn extract, thioglycolic acid, a source of nitrogen and water, characterized in that, in order to increase the yield of spores with high thermal stability, as a source of nitrogen (19) (I) I / 12406 Ai it contains pancreatic meat hydrolyzate and dry casein hydrolyzate in the following ratio of components, g / l: Casein Hydrolyzate Dry 16.5-18.5 Pancreatic meat hydrolyzate (200 · 10 ' ³ % amino-ammonia nitrogen) 4504550 Fodder yeast 19-21 Corn extract 19-21 5 Thioglycolic acid 0.19-0.21 Water Up to a liter Table! Culture medium Kinds hydrolysates. Meat hydrolyzate Casein Hydrolyzate A mixture of hydrolysates meat and casein "The amount of amino-ammonia nitrogen in 10” ³ ,% 130 170 200 250 130 170 200 250 130 170 200 250 Culture growth rate ++++ +++ +++ + +++ +++ +++ + + -H- + -H- Ή- + The number of heat-resistant spores 1 cm ³ - ^; No with 10 * <10 * <w ⁴ Not SU ⁴ 1,5-1 cfilO ⁴ Not sy ⁴ 1 '1 / <10 ⁴ Table The results of spore formation on a nutrient medium with a different ratio of its constituent components The composition of the nutrient medium Casein Hydrolyzate fifteen Meat hydrolyzate (20ΟΊ0 ^-3 % amino-ammonium nitrogen nitrogen) 425 Fodder yeast 18 Corn extract (d.v. 48%) 18 Thioglycolic acid j 0.18 Water ' Up to 1l Spore yield with 1 cm ³ <10 ^-4 The concentration of substances, g / l 16.5 17.5 18.5 21 > 450 500 1. 550 590 19 20 21 22 19 20 21 22 0.19 0.2 0.21 0.22 Up to 1 liter Up to 1 liter Up to 1 liter Up to 1l 10 ⁶ -10 ⁷ 10 ⁶ -10 ⁷ 10-10 ⁷ <10 ^-4