SU170898A1 - - Google Patents
Info
- Publication number
- SU170898A1 SU170898A1 SU892522A SU892522A SU170898A1 SU 170898 A1 SU170898 A1 SU 170898A1 SU 892522 A SU892522 A SU 892522A SU 892522 A SU892522 A SU 892522A SU 170898 A1 SU170898 A1 SU 170898A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- strain
- diameter
- round
- citric acid
- aspergillus niger
- Prior art date
Links
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 15
- 241000228245 Aspergillus niger Species 0.000 description 5
- 235000013379 molasses Nutrition 0.000 description 4
- 230000003042 antagnostic Effects 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K Tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000005557 antagonist Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229910052500 inorganic mineral Chemical class 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L mgso4 Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 239000011707 mineral Chemical class 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N D-sucrose Chemical class O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L Iron(II) sulfate Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- CZMRCDWAGMRECN-GDQSFJPYSA-N Sucrose Natural products O([C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O1)[C@@]1(CO)[C@H](O)[C@@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-GDQSFJPYSA-N 0.000 description 1
- 235000006085 Vigna mungo var mungo Nutrition 0.000 description 1
- 240000005616 Vigna mungo var. mungo Species 0.000 description 1
- NOWKCMXCCJGMRR-UHFFFAOYSA-N aziridine Chemical compound C1CN1 NOWKCMXCCJGMRR-UHFFFAOYSA-N 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 231100000676 disease causative agent Toxicity 0.000 description 1
- 238000005755 formation reaction Methods 0.000 description 1
- 229910000358 iron sulfate Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000877 morphologic Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000019798 tripotassium phosphate Nutrition 0.000 description 1
Description
Известно, что нлесневые грибы рода Aspergillus niger вл ютс возбудител ми процесса нри получении лимонной кислоты биохимическим методом, которую можно производить поверхностным или глубинным способом.It is known that the fungus of the genus Aspergillus niger is the causative agent of the process of obtaining citric acid by a biochemical method that can be produced by surface or deep method.
в основном ее получают первым - поверхностным способом, использу дл этого Aspergillus niger штамм 90. Дл этой же цели - полученп лимонной кислоты поверхностным способом предложен Aspergillus niger штамм ЭУ119 , полученный под вли нием комбинированного воздействи этиленимина и ультрафиолетовых лучей на штамм 90.it is mainly obtained by the first surface method using Aspergillus niger strain 90 for this purpose. For the same purpose obtained by citric acid, the Aspergillus niger strain EU119, obtained under the influence of the combined effect of ethyleneimine and ultraviolet rays on the strain 90, was proposed by the surface method.
Штамм ЭУ-119 отличаетс от исходного штамма большей активностью образовани лимонной кислоты из сахара мелассы и значительно большей устойчивостью к бактери м антагонистам, инфицирующим бродильные среды в производственных услови х. При использовании Aspergillus niger штамм ЭУ-119 выход лимонной кислоты по сравнению с выходом ее при использовании штамма 90 увеличиваетс в среднем на 43% и составл ет 70-75%.The EU-119 strain differs from the original strain in greater activity of citric acid formation from molasses sugar and a significantly greater resistance to bacteria to antagonists that infect fermentation media under production conditions. When using Aspergillus niger strain Eu-119, the yield of citric acid in comparison with the yield of it using strain 90 increases on average by 43% and is 70-75%.
Строение культуры (гигантской колонии) изучали на следующих иитательных средах: Чапека-Докса с добавлением сахарозы и минеральных солей - азотнокислого натри , фосфорнокислого кали , сернокислого магни , The structure of the culture (giant colony) was studied on the following media: Chapek-Doksa with the addition of sucrose and mineral salts — sodium nitrate, potassium phosphate, magnesium sulfate,
хлористого кали и сернокислого железа с добавлением агара; сусло-агаре, приготовленном из неохмеленного иивного сусла; мелассе, подкисленной серной кислотой.potassium chloride and iron sulfate with the addition of agar; Wort agar prepared from unhooked iivnij mash; molasses, acidified with sulfuric acid.
Предмет изобретени Subject invention
Aspergillus niger штамм ЭУ-119Aspergillus niger strain EU-119
Культуральные свойства Минеральна среда Чапека-До кс а. П тисуточна гигантска колони кругла диаметром 4,0 см. Край колонии ровный. Субстратный мицеллий радиально-складчатый. Воздушный мицеллий высокий. Конидни темнокорнчневые . Край колоний, лишенный зрелых конидий, равен 0,7 см.Cultural properties. Mineral environment of čapek-Dox and. There is a giant colony round with a diameter of 4.0 cm. The edge of the colony is even. Substrate mycelium radially folded. Aerial mycelium high. Conidney dark brown. The edge of the colonies, devoid of mature conidia, is 0.7 cm.
Сусло-а га р. П тисуточна гигантска колони кругла , пушиста диаметром 7,3 слг. Конидиальные головки образуютс на значительном рассто нии одна от другой. Край колоний , лишенных зрелых конихТ.ий, равен 1,1 см.Wort-a ha p. There is a giant colony round, with a diameter of 7.3 slg. Conidial heads are formed at a considerable distance from one another. The edge of the colonies devoid of mature horses. 1.1 cm.
Меласса. Мицеллий формируетс на иоверхности среды в виде складчатой пленки кремового цвета.Molasses. A micellium forms on the surface of the medium as a cream-colored folded film.
Морфологические свойства Среда Чапека-Докса. У п тисуточной культуры конидиальные головки круглой или звездчатой формы диаметром 85-190 (д,. Преобладают головки диаметром 105-115 ji. Пузырьки круглой и выт нутой формы диаметром 34-35 р,. Стеригмы двухслойные. Длина стеригм первого пор дка 11 |л, второго 7,5 |Li. Конидип круглые, их средний диаметр 3,4 Л. Длина конидиеносцев от 0,7 до 5 мм.Morphological properties of the Чapek-Doksa environment. The five-day culture has round or star-shaped conidial heads with a diameter of 85-190 (d., Heads with a diameter of 105-115 ji prevail. Bubbles of a round and elongated shape with a diameter of 34-35 r., Sterigms are double-layered. First-order sterigm length 11 | L , the second 7.5 | Li. Conidip are round, their average diameter is 3.4 L. The length of conidiophores is from 0.7 to 5 mm.
Антагонистические свойстваAntagonistic properties
: Обладает более высокой устойчивостью к бактери м, антагонистам, инфицирующим бродильные среды.: It has a higher resistance to bacteria, antagonists that infect fermentation media.
Сбраживание СахаровFermentation of Sakharov
Хорошо сбраживают мелассу при поверхностном способе культивировани , увеличива выход лимонной кислоты.Molasses is well fermented with a superficial method of cultivation, increasing the yield of citric acid.
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU170898A1 true SU170898A1 (en) |
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