SU1696999A1 - Method for detecting atropine sulfate - Google Patents

Abstract

This invention relates to analytical chemistry, in particular to the determination of atropine sulfate. The goal is to increase the accuracy and selectivity of the determination. Determination is carried out by titration of the analyzed sample in a mixture of alcohol and chloroform with an alkali solution in the presence of an indicator, ethacridine lactate. 7 tab.

Description

This invention relates to analytical chemistry, in particular to a method for determining a salt of a strong organic base, atropine sulfate.

The aim of the invention is to improve the accuracy and selectivity of the determination.

Example 1: Method for determination of atropine sulfate (in a 1% solution).

In a wide tube or measuring cylinder (2 cm in diameter), 2 ml of the test solution, 4 ml of chloroform, 1 ml of ethanol, 4 drops of a 1% solution of ethacridine lactate and 1 drop of a phenol red solution are placed (against the background created by the presence of phenol red, the reddish color of the upper aqueous layer is more clearly observed the color of the lower organic layer at the equivalent point) and the liquid is slowly, dropwise, titrated with 0.1 n. sodium hydroxide solution before staining the chloroform layer yellow. 1 ml 0.1 n. sodium hydroxide solution corresponds to 0.0347 g of atropine sulfate.

The results of the quantitative determination of atropine sulfate are presented in Table 1.

PRI mme R 2. Analysis of eye drops No. 1, containing,%: atropine sulfate 0.1 and a solution of boric acid 2% 10.0.

Separate alkalimetric determination of both ingredients in this mixture is difficult, since atropine sulfate is partially titrated during the titration of boric acid over phenolphthalein in the presence of glycerol. Alkalimetric titration of boric acid in the presence of sorbitol forming a stronger acid allows titration with phenol red. In this case, atropine sulfate is not titrated, and the use of an extractable indicator, ethacridine lactate, does not interfere with

In the presence of conventional indicators that change the color in the aquatic environment, the alkalimetric determination of boric acid

o you o you you

you and atropine sulfate with the joint presence is almost impossible.

Quantitation,

Boric acid 2 ml of the test solution are placed in a measuring cylinder of 25 ml; 1.0 sorbitol is dissolved, 1 drop of phenol red is added, 0.5N is added. sodium hydroxide solution in an amount approximately equal to the content of 90% boric acid, i.e. 1.2 mp (Yi). Then continue to titrate with 0.1 n. sodium hydroxide solution to pink color (Ya). 1 ml of 0.1 N sodium hydroxide solution corresponds to 0.008184 g of boric acid.

To a titrated liquid, add 4 ml of an alcohol: chloroform mixture in a ratio (1: 4), 4 drops of a 1% solution of ethacridine lactate and titrate with 0.1N. sodium hydroxide solution, as indicated, until the yellow color of the organic phase (Uz). 1 ml 0.1 n. sodium hydroxide solution corresponds to 0.0347 g of atropine sulfate.

The content of boric acid in the dosage form (in grams) is calculated by the formula

Y2

(Yi + -) -0.03092

ten

}

where YI is 0.5 n. sodium hydroxide solution added to bind boric acid, ml;

Y2 is the amount of 0.1 n sodium hydroxide solution consumed for titration of boric acid, ml.

The content of atropine sulfate in the dosage form (in grams) is calculated by the formula

X

 Y3 0.0347 10

where Ouse - the amount of 0.1 n. sodium hydroxide solution consumed for the titration of atropine sulfate, ml.

The results of quantitative determination of the ingredients in eye drops No. 1 are presented in Table 2,

Eye drops number 2,%: a solution of atropine sulfate 1 and ethyl morphine hydrochloride 1.

2 ml of the test solution, 4 ml of chloroform and 1 drop of the phenol red indicator solution are placed in a wide test tube or a measuring cylinder (2 cm in diameter), and the liquid is titrated with shaking 0.1N. sodium hydroxide solution before pinking the aqueous layer. one

ml 0.1 n. sodium hydroxide solution corresponds to 0.03859 g of ethyl morphine hydrochloride.

Then, 1 ml of ethyl alcohol, 4 drops of a 1% solution of zacridine lactate are added to the liquid and titrated dropwise with shaking of 0.1N. sodium hydroxide solution to a yellow color of the chloroform layer. 1 ml 0.1 n. Sodium hydroxide solution (consumed during the second titration) corresponds to 0.03474 g of atropine sulfate.

The results of quantitative determination of ingredients in eye drops No. 2 are presented in Table 3. Atropine Determination Results

sulfate on the prototype method are presented in table 4.

The results of the determination of atropine sulfate by the proposed method are presented in Table 5.

These tables show that the tendency to obtain higher results (100%) can be traced by the prototype method, which can be explained by the influence on the prototype method, in contrast to the proposed carbon dioxide of air, when using weaker (0, 02 n.) Titrated solution of NaOH, in the presence of a phenolphthalein indicator that is sensitive to weak acids.

The use of phenol red in the titration of individual solutions of atropine sulfate is optional, although its presence stains the upper layer red and thereby improves the observation of the equivalent point, practically not reflecting on the results.

The results of the determination of atropine sulfate by the proposed method in the presence of phenol red are presented in Table 6.

The results of the determination of atropine sulfate by the proposed method in the absence of the red phenologist are presented in Table 7.

Thus, the invention makes it possible to increase the accuracy and selectivity of the determination.

Claims (1)

Claims The method of determining atropine sulfate by titrating the sample to be analyzed in a mixture of alcohol and chloroform with an alkali solution in the presence of an indicator, is due to the fact that ethacridine lactate is used as an indicator to increase the accuracy and selectivity of the determination. Table 1 table 2 Table 3 Table 4 Table 5 Table 6 Table 7