SU1695869A1 - Method for preparation biostimulator "splenivite" of animal spleen - Google Patents

Abstract

The invention relates to agriculture. The purpose of the invention is to increase the effectiveness of the biostimulant Splenivit. The method of obtaining a biostimulant from the spleen of animals includes pulp grinding, extraction with saline, heat treatment, sediment separation, filtration and sterilization, and after pulp grinding it is frozen at a temperature of (-) 18.0-20 ° C for 24 hours and defrosted for 4-6 hours at 18-20 ° C, pulp extraction is carried out with buffered saline in an acidic medium at pH 4.4 in equal ratios of pulp and saline, and after separating the precipitate, the resulting filtrate is further filtered and sterilized in apparatus C lnikova and vitamin 1V12 was added in an amount of 40 g per 1 liter of the resulting formulation. When testing plepivitis on the bodies, a reduction in the incidence of morbidity by 10% and an increase in average daily weight gain by an average of 10 g per head was found. When tested on pigs, a decrease in the mortality of animals was observed by 7–13.8% and an increase in weight gain by 38.8 g compared with the control. The use of splenivite in pregnant sows contributes to the reduction of dead pigs by 6.6%, increasing the average daily weight gain by 20.7 g and the yield of piglets by 2.1 piglets from each sow. (Ls

Description

This invention relates to agriculture.

The purpose of the invention is to increase the effectiveness of the drug.

Example 1. At the m sombinate during the slaughter of healthy animals or no later than 2 hours after the nutting, 10 kg of the spleen are selected. They are cleared of fat and placed in a refrigerator, kept for 5-6 days at T 2-4 ° C, or can be frozen in a chamber with T () ° C and stored for a month.

After 5-6 days, the spleen is washed with water, the capsule is removed, and the pulp is homogenized. To increase the biological activity of the cells, the homogenized tissue is frozen at T (-18H-20) ° C for 24 hours, then slowly at room temperature 18-20 ° C is defrosted (thawed), and then filled with buffered saline in a 1: 1 ratio (plus 1 Buffered saline solution (boiling point), boil at 100 ° C for 1 h, cooled to 40-50 ° C. Ballast materials are removed by filtration. The filtered solution is additionally passed through filtering-sterilizing plates in the apparatus.

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Salnikov. For filtration and sterilization use a three-frame filter FS-3.

The main parameters of the filter: working pressure inside the filter KPA (kgf mm2) - 20 (0.2); number of frames - 3; overall dimensions, mm: in plan 360,334. height 165. Diameter of plates GOST 480 × 68, mm: filtering 300, sterilizing 300; plate thickness, mm: filtering 3-4, sterilizing 4. Capacity of plates, l / hm2: filtering 1920, sterilizing 77. Vitamin 812 is added sterilely at the rate of 4000 µg per 100 ml of filtrate, packaged in vials.

Check pH, sterility, toxicity (0.3 ml s / c 10 white mice, observe for 7 days - mice should be healthy).

The buffered solution is prepared in the ex- ampore and consists of one part of monosubstituted potassium phosphate (9.006 per 1 liter of distilled water. PH 4.4) and 9 parts of saline (Og85% sodium chloride).

The biological activity of the substance isolated from the spleen was evaluated by the increase in body weight and the ability to increase the number of antibody-forming cells in the spleen of experimental animals.

Example 2. Experiments conducted on white mice weighing 20 g; the animals were divided into three groups: the control and two experimental ones.

The animals of the first experimental group were intraperitoneally injected with 0.2 ml of a 1:10-diluted preparation obtained from the spleen by the proposed method.

Animals of the second experimental group were injected intraperitoneally with 0.2 ml of 1:10 diluted tissue preparation obtained from the spleen according to V.P. Filatov (known method).

Animals of the control group were injected, 0.2 ml of saline intraperitoneally.

After 1 day, 0.2 ml of a 25% suspension of sheep erythrocytes was intraperitoneally injected into all the groups of animals.

Zabdny weight of animals in the control group increased by an average of 0.65 g, the first one experienced by 1.23 g, the second one experienced by 0.8 g.

After 5 days, the animals of all groups were killed. In the spleen, taken from them, the number of antibody-forming cells (AOK) was determined by local hemolysis.

It was established that in control animals there were 59U2 AOK per 10 million splenocytes. In animals of the first experimental AOK group, in animals of the second experimental group 80 AOK.

Thus, the proposed drug has tissue-specific stimulation of immunocompetent cells in the spleen and is significantly superior in its properties to the tissue preparation.

Experiments were performed on calves, pigs, and sows.

PRI me R 3. Animals were divided into eight groups: control and experimental.

Experimental animals were injected three times with an interval of 7-10 days in the resulting preparation Splenivit in doses, ml: pig: there:

up to 1 month age 0.2 0.2 0.3

older than 1 month 1.0 1.0 1.0-1.5

1.0 1.5 1.5 2.0 2.0 3.0

sows 30, 20, 10 days before farrowing 5.0 5.0 5.0

The results of the experiment obtained the following data.

The use of Splenigue bodies there older than 1 month (97 years old) increases their overall resistance, allows for better adaptation to new conditions, reduces the incidence of disease by 10%, thereby increasing the average daily weight gain (951-907 g) compared with the control group ( 107 heads).

After three times intramuscular administration of the drug, the total protein content, serum gamma globulin, its bactericidal activity increased in calves after a three-fold intramuscular administration of the drug. The incidence of respiratory diseases decreased by 1.5 times, and the average daily weight gain increased by an average of 40 g per head.

Application of Splenivitis to newborn piglets there (307 heads (contributed to a decrease in mortality by 7% (18.7-11.7%)) culling them to 35 days of age by 13.8% (40.9-27.1) and an increase in average daily weight gain by 38.8 g (206-167.2 g) compared with the control group (336 heads). The application of splenivite to fattening pigs increases their resistance to diseases, reduces culling by 13%, and increases the average daily gain by 50 g compared to with pigs that Splenivit did not use.

The use of splenivit to pregnant sows contributes to their increased resistance, reducing the birth of dead pigs by 6.6%, improving the preservation of burrows

in the well-developed pigs to weaning on the effectiveness of the drug, after grinding 15.8%, increasing the average daily consumption of the pulp is frozen at a temperature of –g and the output of the pigs by 2.1 pigments -18-20 ° C, for days and ka from each sow. thawed for 4-6 hours at a temperature of 18520 ° C, and the extraction of the pulp is carried out

Claims (1)

Claims of the invention., Buffered saline in an acidic medium at pH 4.4 in equal ratios of pulses. A method for producing a biopsy stimulator and saline, and after separating the precipitate. spleen of animals, including the ground-obtained filtrate additionally filtering the pulp, extracting it to a physical growth and sterilizing it in the Salnikov apparatus thief, heat treatment, separation and add vitamin B 12 in the amount of 40 g sedimentation, filtration and sterilization, about t l and-1 l of the obtained preparation, which is aimed at increasing