SU1681266A1 - Method for determining the binding capacity of albumin in the blood serum - Google Patents

Abstract

This invention relates to medicine. The purpose of the invention is to simplify and speed up the method. The essence of it is that fluorescence of 4-substituted 1,8-naphthalene dicarboxylic acid fluorescent N-carboxyphenyl imides is measured by fluorescence. For analysis, 0.01 ml of serum is sufficient. The dye consumption is 11 mg per 1000 analyzes. Duration of determination is no more than 3.5 minutes. 3 tabl

Description

45

Table 1

Fi and Fa-fluorescence intensity of PS in blood sera, divorced X times, with albumin content of 51 and 34 g / l, respectively.

) Mean ± mean error

Process steps

According to the proposed method

1. Blood serum production

2. Add FS

3.Mixing

4. Measurement of fluorescence intensity relative to reference

5. Evaluation of deviations from the norm. Total:

According to the method prototype

1. Preparation of Kral working solution

2. Add a dye solution to the sylvka

3. Incubation at 37 ° C

4. Add saturated sodium hydroxide solution.

5. Mixing

6. Incubation at 37 ° C

7. Mixing

8. Filtration

9.Production

10. Measurement of optical density

11. Evaluation of deviations from the norm. Total:

table 2

Table 3

Duration, min

0.5 0.3 0.2

2.0 0.5 3.5

0.5

0.5 30.0

0.3

0.2 1440.0

0.2

5.C

0.3

1.0

0.5 1478.5

Claims (1)

Claim A method for determining the binding capacity of albumin in serum by adding to a diluted serum a substance interacting with albumin, followed by measuring the amount of product obtained and comparing its level with a control serum, characterized in that what. in order to simplify and speed up the method, as a substance interacting with albumin, use is made of a compound of the general formula where R is N (CH ₃ ) 2; N (C ₂ H5) 2: Ri-H.COOH; R2 - OH, COOH, blood serum is diluted 120-2000 times, the compound is added at a concentration of 2402 / X - 3600 / X μM, where X is the serum dilution, and the fluorescence intensity is measured in the region of maximum luminescence of the resulting solution. Table 1 F * FS concentration, μM 600 / X 1200 / X 1800 / X 2400 / X 3000 / X 3600 / X 4200 / X Fi 35 56 64 67 67 67 66 F2 25 33 37 38 38 38 37 * Fi and F ₂ fluorescence intensities of PS in blood serum diluted X times with an albumin content of 51 and 34 g / l, respectively. table 2 Examinees Number of observations Binding capacity * albumin (CEA),% The range of changes in CEA (the proposed method),% the proposed method prototype Healthy Brlny with hyperbilirubinemia and with purulent-septic disease 20 100 ± 2 100 ± 2 85-111 vanyami 22 39 ± 4 40 ± 4 20 - 82 *) Mean ± error of the mean Table 3 Process stages Duration min According to the proposed method 1. Dilution of blood serum 0.5 2, Adding FS 0.3 3. Stirring 0.2 4. Measurement of fluorescence intensity relative to the standard 2.0 5. Assessment of deviations from the norm 0.5 Total: 3.5 According to the prototype method 1, the preparation of the working solution paint- a la 0.5 2. Adding a dye solution to serum ke 0.5 3. Incubation at 37 ° C 30,0 4. Adding a saturated solution of chloro- sodium sodium 0.3 5. Mixing 0.2 6. Incubation at 37 ° C 1440.0 7. Stirring 0.2 8. Filtering 5.C 9. Breeding 0.3 10. Measurement of optical density 1.0 11. Assessment of deviations from the norm 0.5 Total: 1478.5