SU1565881A1 - Apparatus for growing microorganisms - Google Patents

Abstract

The invention relates to the microbiological industry, in particular to apparatus for growing microorganisms. The aim of the invention is to increase the productivity of the apparatus by increasing the circulation ratio of the medium. The device contains a tank 1 with a lid, a cylinder 3 installed along its center with the formation of an annular space divided by two partitions 4 and 5 into the growing zone 6 and the ripening zone 7, each of which consists of sections 8 and turbines 9 placed in them, diffusers 10 and cups 11. The walls of the diffusers 10 in the growing zone 6, except for the walls of the last (IX) section in the course of the process, and the walls of the glass 11 in this section are attached to the lid of the tank 1 with the formation of channels 13-15, while the last (IX) section contains additional partitions 16 for additional The flow of the medium from channels 13 and 15 into channel 14. In zone 7 of ripening walls of cups 11 and diffusers 10, channels 17–19 are also formed to circulate the medium, with the last (XII) section in this zone provided with partitions 20 and 21 for to ensure the flow of the medium in these channels, the apparatus is also equipped with nozzles 22 and 23 for feeding the nutrient medium and draining the target product, respectively. The proposed apparatus allows to increase the average flow rate of the medium and the multiplicity of circulation, thereby increasing the oxygen content in the environment and more fully utilize the components of the nutrient medium. 4 il.

Description

the walls of the cups 11 and the diffusers 10 also formed channels 17–19 for the circulation of the medium, while the last (XII) section in this zone is equipped with partitions 20 and 21, ensuring the flow of the medium in these channels is also equipped with nozzles 22 and 23 for feeding nutrient

environment and removal of the target product, respectively. The proposed apparatus allows to increase the average flow rate of the medium and the multiplicity of circulation, thereby increasing the oxygen content in the environment and more fully utilize the components of the nutrient medium. 4 il.

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The invention relates to the microbiological industry, anna-f5 atam for growing microorganisms.

The aim of the invention is to increase productivity due to the magnitude of the circulation ratio of the cycle.

FIG. 1 shows the apparatus, a top view in section; in fig. 2 shows section A-A in FIG. one; in fig. 3 is a section bb of fig 1; in fig. 4 through 25 section BB in FIG. 1 (numbers I - XIII denote the sequence of sections along the process),

The apparatus for growing microorganisms contains a cylindrical jQ tank 1 with a lid 2, mounted on the center of the tank 1 cylinder 3 with the formation of an annular space between the walls of the cylinder 3 and tank 1, divided by two radial partitions 4 and 5 into the growing zone 6 and the maturation zone 7 of microorganisms, each of which consists of interconnected sections 8 and placed.,. they have self-priming turbines 9, diffusers 10 and circulation cups 11, between which heat exchangers 12 are installed. Walls of diffusers 10 in sections 8 located in zone 6, the cultivation of microorganisms, except the walls of diffuser 10 of the last section (IX) during the process and the walls of the cup 11 in this section are attached to the lid 2 with 5–5 bone 1, forming channels 13–15 for the medium to circulate in the horizontal plane between the walls of the tank 1, the diffusers 10 and the cylinder 3, with the last section (1X) in the course of the process, in voche 6 grows and further comprising radial baffles 16 attached to the lid 2 of the container 1 placed h

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between the walls of the glass 1 1 and the diffuser 10 to ensure the flow of the medium from the channels 13 and 15 into the channel 14. In zone 7 of maturation (in sections X, XI and XII) the walls of the glasses 11 of sections 8 located in this zone, except for the walls of the glass 11 The last sections 8 in the course of the technological process and the walls of the diffuser 10 in this last section are attached to the lid 2. The capacitance 1 with the formation of channels 17-19 for the circulation of the medium in a horizontal plane, as in the growing zone 6, between the walls of the tank 1, the cups 11 and cylinder 3, while the last section (XI) along the process in zone 7 ozrevani contains radial partitions 20 and 21, attached to the lid 2 of the container 1 and measured between the diffuser walls 10 and the walls of the cup 11 to provide a flow medium from the channel 18 into the channels 17 and 19.

The apparatus is also provided with a nozzle 22 for feeding the nutrient medium and a nozzle 23 for withdrawing the target product.

The device works as follows.

According to the technological regulations in the apparatus, with the turbines 9 turned on, disintegration, cultivation of microorganisms, maturation in the corresponding sections 8 and zones 6 and 7 of the tank 1 are carried out to obtain a yeast suspension. To create optimal conditions for these processes, the culture medium is aerated and mixed in each section 8 using self-priming turbines 9. Rotated from electric motors (not shown), each turbine 9 draws air from the atmosphere, draws culture liquid through the upper and lower necks, mixes it with by air and

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throws the gas-liquid mixture into the circulation channels under the heat exchangers 12. Pass through the heat exchangers 12, the culture fluid rises to the top edge of the stack 11 or diffusers 10, flows over them and lowers outside one of their walls, a part of it re-enters into one of the suction and the other part is directed to the next section 8 and partly mixed with the culture fluid ejected from the annular nozzle of the adjacent turbine 9, and directed further along the wall of the tank 1, and partly into one of the necks of the adjacent turbine 9 is sucked in. A fresh nutrient mixture containing the substrate, nutrient salts and trace elements in a solution of water is continuously fed through pipe 22 into the central section (XIII); in the first section 8 of the growing zone 6, where the turbine lower throat 9 is sucked in. Since in the growing zone 6 in eight sections of the walls of the diffusers 10, and in the ninth and last section of the walls of the glasses 11, they are raised significantly above the working level of the culture liquid in the apparatus, The upper necks of the turbines 9 in all sections are separated in liquid from their lower necks. The capacity of each turbine 9 in liquid is 3000 m3 / h, therefore, one of its necks can provide liquid in the amount of 1500 m / h. The remaining 1500 m / h of fluid ejected from each turbine 9 into channels 13 and 15, enclosed between the wall of the tank 1 and the walls of the cups 11, as well as between the wall of the cylinder 3 and the walls of the cups 11, rush along the indicated annular channels of the tank to the last ninth (Ix) sections 8 of the growing zone 6, where this excess liquid is sucked in by the lower neck of the turbine 9 and discharged together with the volume of liquid flowing through its upper throat into the interior of the ninth (IX) section in an amount of about 3000 m5 / h. Due to the fact that the volume in the ninth section is connected along the level of a liquid with an annular channel 14 enclosed between the walls of diffusers 10, about half the volume of liquid

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the bones produced by the turbine 9, the ich of the ninth section are continuously fed into the specified annular channel 14, for one hour it is sucked in by the upper necks of the turbines 9 located in this annular space. The remaining part of the culture fluid, due to the resulting difference in the level of liquid between the first (I) and ninth (IX) sections of the growing zone, flows at a high speed through channel 14 in the direction of the first section (I again and is involved in the circulation.) 8, the excess culture liquid is disassembled by the upper necks of the turbine 9 in an amount of about 1500 m3 / h so that in the first section the excess of the liquid level of the growing zone 6 above the liquid level in the ripening zone 7 is established. a continuous flow of the culture liquid from the growing zone 6 to the ripening zone 7 goes in an amount corresponding to the amount of fluid flow through the apparatus. In the ripening zone 7, the circulation of the culture fluid proceeds in the same way as in the growing zone 6, with the only difference being that directed to the central channel 18 of the circulation loop formed by the walls of the cups 11 and the walls of the diffusers 10 in the last section 8 of the growing zone 6. From the last section (IX), the liquid is ejected by the turbine 9 into the peripheral circulation channel 17 and is directed from the radial partitions 20 and 21 to the partition 4, moving in the channels 17 and 19 between the walls of the tank 1 and the glasses 11, as well as between the wall of the cylinder 3 and the walls of the cups 11. A portion of the finished product in the form of a yeast suspension is continuously withdrawn from the zone of ripening through the nozzle 23.

Due to the creation in circulation zones 6 and maturing 7 of channels 13-15 and 17-19 of the circulation of culture liquid (central 14 and 18 peripheral 13, 15 and 18, 19), the total length of the circulation flow path B of the apparatus doubles. In this case, due to the fact that the entire volume of fluid in the tank 1 is pumped by the turbines 9 along circuits having a cross section

smaller than the cross section of the entire annular space of the vessel 1, the average velocity of the gas-liquid flow in the annular space of the apparatus increases significantly.

Due to a significant increase in the average velocity of gas-liquid flows, the apparatus reduces the harmful effects of stagnant zones and eliminates bypass liquid flows. As a result, the content of dissolved oxygen and the substrate in the culture fluid at any point of the growing area becomes the same, i.e. The conditions of growing microorganisms in all sections of the apparatus are equalized and become optimal, which provides an increase in the productivity of the apparatus as a whole and an improvement in the quality of the finished product with respect to the permissible content of hydrocarbons in the nominal

Claims (1)

Invention Formula An apparatus for growing microorganisms, containing a cylindrical tank with a lid, mounted along to the center of the cylinder, forming an annular space between the walls of the cylinder and the container divided by two radial partitions into the cultivation area and the maturation zone of microorganisms, each of which consists of interconnected sections and self-priming turbines placed in them, diffusers and circulation glasses, between which heat exchangers, characterized in that, in order to increase productivity by increasing the multiplicity of medium circulation, the walls of diffusers in sections placed in After growing, in addition to the walls of the diffuser from the last section along the process and the wall of the glass in this section, they are attached to the lid of the container to form channels for the circulation of the medium in the horizontal plane between the walls of the container, the diffuser and the cylinder, the latter section containing additional radial partitions attached to the lid of the vessel, placed between the walls of the glass and the diffuser for the flow of medium from the channel into the channel. Heaven 2 eleven Rig. H Compiled by N. Osipov Editor O. Yurkovetska Tehred M. Khodanych Urobenia mud- bones. Proofreader T.Paly